Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD92 (VIM15) PE antibody (10ul reagent/100ul of peripheral whole blood).)
Mouse anti-Human CD92 Antibody | anti-CD92 antibody
Anti-Hu CD92 PE
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Synonyms
CD92; Anti-Hu CD92 PE; CTL1; CHTL1; SLC44A1; solute carrier family 44 member 1; anti-CD92 antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG1
Clone Number
VIM15
Specificity
The mouse monoclonal antibody VIM15 recognizes an extracellular epitope of CD92, a 70kDa transmembrane glycoprotein expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines.
Purity/Purification
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
Applicable Applications for anti-CD92 antibody
Flow Cytometry (FC/FACS)(QC tested)
Application Notes
FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests.
Conjugation
PE
Immunogen
MV4-11 acute myeloid leukemia cells
Excitation Laser
blue (488 nm)
Workshop
HLDA VI: M-MR6, HLDA V: BP-BP229, M-M15
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD92 (VIM15) PE antibody (10ul reagent/100ul of peripheral whole blood).)
Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD92 (VIM15) PE antibody (10ul reagent/100ul of peripheral whole blood).)
Flow Cytometry (FC/FACS)
(Separation of human CD92 positive monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD92 (VIM15) PE antibody (10ul reagent/100ul of peripheral whole blood).)
Flow Cytometry (FC/FACS)
(Separation of human CD92 positive monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD92 (VIM15) PE antibody (10ul reagent/100ul of peripheral whole blood).)
Related Product Information for anti-CD92 antibody
CD92 is a 70kDa protein with ten transmembrane domains, intracellular N and C teminus, and two glycosylated larger extracellular loops. In the C-terminal domain, there is an ITIM-like sequence. This protein seems to be a choline transporter responsible for delivery of choline into the immune cells, to make it accessible for phospholipid synthesis, as well as a regulator of immune cell signaling. It is expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines. It can also be found on mast cells (but not eosinophils), and weakly on peripheral blood lymphocytes, fibroblasts, epithelial cells, and endothelial cells.
References
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Wille S, Szekeres A, Majdic O, Prager E, Staffler G, Stöckl J, Kunthalert D, Prieschl EE, Baumruker T, Burtscher H, Zlabinger GJ, Knapp W, Stockinger H: Characterization of CDw92 as a member of the choline transporter-like protein family regulated specifically on dendritic cells. J Immunol. 2001 Nov 15;167(10):5795-804., URL: https://www.jimmunol.org/content/167/10/5795.long
Wille S, Szekeres A, Majdic O, Prager E, Staffler G, Stöckl J, Kunthalert D, Prieschl EE, Baumruker T, Burtscher H, Zlabinger GJ, Knapp W, Stockinger H: Characterization of CDw92 as a member of the choline transporter-like protein family regulated specifically on dendritic cells. J Immunol. 2001 Nov 15;167(10):5795-804., URL: https://www.jimmunol.org/content/167/10/5795.long
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Product Notes
The CD92 (Catalog #AAA1802267) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD92 PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD92 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS)(QC tested). FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests. Researchers should empirically determine the suitability of the CD92 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD92, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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