$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Testing Data (Immunocytochemistry stain of Hela using AKR1B1 antibody (1:300).)
Testing Data #2 (Western blot detection of AKR1B1 in HT1080, MCF7, A431&Hela cell lysates using AKR1B1 antibody (1:1000 diluted). Predicted band size:36KDa. Observed band size:36KDa.)
Testing Data (Western blot detection of AURKA in HL60, SW480, MCF7, 293, COS7 & HepG2 cell lysates and using AURKA antibody (1:200 diluted). Predicted band size: 46KDa Observed band size:46KDa.)
SDS-PAGE
SDS-PAGE (The purity was determined to be >85% by densitometry. Approx.MW 74 kDa.)
Testing Data (Immunocytochemistry of HeLa cells using anti- BiP/GRP78 (C-terminus) antibody diluted 1:100.)
Testing Data #2 (Western blot detection of BiP/GRP78 (C-terminus) in Hela, LNCAP,C6&MCF7 cell lysates using BiP/GRP78 (C-terminus) antibody (1:1000 diluted). Predicted band size: 72kDa Observed band size: 72kDa.)
Dot Blot (DB) (Dot blot analysis of mouse TSC2 Antibody (Phospho S1343) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Tissue: transfected HeLa cells. Species: Human. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Robert H Edwards, U. of Cali, San Fran School of Medicine.)
Western Blot (WB) (Western Blot analysis of Rat liver microsome lysate showing detection of LAMP1 protein using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Western Blot (WB) (SLAMF6 Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SLAMF6 antibody detected the SLAMF6 protein (arrow).)
Testing Data (Western blot detection of CKMT1 in Mouse Colon,293&A431 cell lysates using CKMT1 antibody (1:1000 diluted). Predicted band size:47KDa Observed band size:47KDa.)
Testing Data (Western blot detection of SMARCA4 in K562&HelaNE cell lysates using SMARCA4 antibody (1:1000 diluted). Predicted band size: 235KDa Observed band size: 235KDa.)
Testing Data (Immunocytochemistry of HeLa cells using anti- CDK5(N-terminus) antibody diluted 1:150.)
Testing Data #2 (Western blot detection of CDK5(N-terminus) antibody in Jurkat,K562&Hela lysates using CDK5(N-terminus) antibody (1:1000 diluted). Predicted band size: 36KDa Observed band size: 36KDa.)
Testing Data (Western blot detection of TFIIH (N-terminus) in LNCAP&K562 cell lysates using TFIIH (N-terminus) antibody (1:1000 diluted). Predicted band size: 62KDa Observed band size: 62KDa.)
Testing Data #2 (Western blot detection of TFIIH(N-terminus)in Hela,Hela NE & Jurkat cell lysates using TFIIH (N-terminus) antibody (1:300 diluted).Predicted band size: 62KDa.Observed band size: 62KDa.)
Testing Data (Western blot detection of NCAM-L1(N-terminus) antibody in HepG2 cell lysates using NCAM-L1(N-terminus) antibody (1:1000 diluted). Predicted band size:140KDa Observed band size:140KDa.)
Testing Data (Western blot detection of DCTN1 in A2780, TF-1, HepG2, MCF7,A431&K562 cell lysate(1:1000 diluted). Predicted band size:150KDa Observed band size: 150KDa.)
Testing Data #2 (Immunoprecipitation analysis of Hela cell lysates using DCTN1 antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.)
Western Blot (WB) (Western blot analysis of Mouse, Rat brain cell lysates showing detection of Bassoon protein using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:1000.)
Testing Data (Western blot detection of ALDH3A2 in A549,U251&HepG2 cell lysates using ALDH3A2 antibody (1:1000 diluted). Predicted band size:55KDa Observed band size:55KDa.)
Testing Data (Western blot detection of PRMT1 in Hela&A549 cell lysates using PRMT1 antibody (1:1000 diluted). Predicted band size:42KDa Observed band size:42KDa.)
Testing Data (Immunocytochemistry stain of Hela using HSPA1A antibody (1:300).)
Testing Data #2 (Western blot detection of Hsp70(N-terminus) antibody in Heat shcok Hela,Hela&HepG2 lysates using Hsp70(N-terminus) antibody (1:1000 diluted). Predicted band size: 70KDa Observed band size: 70KDa.)
Western Blot (WB) (Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Rabbit Anti-Ubiquitin Monoclonal Antibody, Clone FK2. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
Testing Data (Western blot detection of PPP3CA in A431&Hela cell lysate (1:1000 diluted) Predicted band size: 59KDa Observed band size: 59KDa.)
Testing Data (Immunocytochemistry stain of Hela using FYB antibody (1:300).)
Testing Data #2 (Western blot detection of FYB in Jurkat, K562&TF-1 cell lysates using FYB antibody (1:1000 diluted). Predicted band size: 85kDa Observed band size: 85kDa.)
Western Blot (WB) (Western Blot analysis in Hela cell line lysates (35ug/lane) uusing of MBS642638. ADIPOR2 (arrow) was detected using MBS642638.)
Western Blot (WB) (All lanes : Anti-ADIPOR2 Antibody (Center) at 1:2000 dilution Lane 1: U-251 MG whole cell lysateLane 2: U-2OS whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot(WB) (Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human liver tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human skeletal muscle tissue lysateLysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of Hela cell using MBS642638 followed by Alexa Fluor489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC) (Flow cytometric analysis of Hela cells (right histogram) using MBS642638 compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Testing Data (Western blot detection of ALB in Human plasma cell lysates using ALB antibody(1:500 diluted).Predicted band size:70KDa,Observed band size:70KDa.)
Testing Data #2 (IHC of paraffin-embedded human liver cancer using anti- Albumin diluted 1/100.)
Western Blot (WB) (GNAQ Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the GNAQ antibody detected the GNAQ protein (arrow).)
Western Blot (WB) (Western blot analysis of GNAQ (arrow) using rabbit polyclonal GNAQ Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAQ gene.)
Testing Data (Immunocytochemistry stain of Hela using RAB4A-N antibody (1:300).)
Testing Data #2 (Western blot detection of RAB4A in Rat stomach, Mouse brain, Hela&MCF7 cell lysates and using RAB4A antibody (1:1000 diluted). Predicted band size: 24KDa Observed band size: 30KDa.)
Testing Data (Immunocytochemistry of HeLa cells using anti- PP2A-alpha (N-terminus) antibody diluted 1:50.)
Testing Data #2 (Western blot detection of PP2A-alpha(N-terminus) antibody in MCF7 cell lysates using PP2A-alpha(N-terminus) antibody (1:1000 diluted). Predicted band size:35KDa. Observed band size:35KDa.)
Testing Data (Immunocytochemistry of HeLa cells using anti- JMJD6(N-terminus) antibody diluted 1:200)
Testing Data #3 (Western blot detection of JMJD6(N-terminus) antibody in Hela&HL-60 lysates using JMJD6(N-terminus) antibody (1:1000 diluted). Predicted band size: 46KDa Observed band size: 62KDa.)
SDS-PAGE
Testing Data (Western blot detection of ANXA6 in T47D,MB4,CEM,MOLT-4 &Jurkat cell lysates and using ANXA6 antibody (1:1000 diluted). Predicted band size: 76KDa Observed band size:76KDa.)
SDS-PAGE
Testing Data (Western blot detection of FOXO1(C-terminus) antibody in A431&Hela cell lysates using FOXO1(C-terminus) antibody (1:1000 diluted). Predicted band size:70KDa Observed band size: 70KDa.)
Testing Data (Immunocytochemistry of HeLa cells using anti-PP1A (N-terminus) antibody diluted 1:50.)
Testing Data #2 (Western blot detection of PP1A(N-terminus) antibody in HepG2,MCF7&Hela cell lysates using PP1A(N-terminus) antibody (1:1000 diluted). Predicted band size:37KDa. Observed band size:37KDa.)
Testing Data (Immunocytochemistry stain of Hela using HK1 antibody (1:300).)
Testing Data #2 (Western blot detection of HK1 in Hela&Jurkat cell lysates using HK1 antibody (1:10000 diluted). Predicted band size: 102kDa Observed band size: 102kDa)
Testing Data (Immunocytochemistry of HeLa cells using anti- KDM5C (C-terminus) antibody diluted 1:150)
Testing Data #2 (Western blot detection of KDM5C(C-terminus) antibody in Hela&293 cell lysates using KDM5C(C-terminus) antibody (1:1000 diluted). Predicted band size: 176KDa Observed band size: 220KDa)
Testing Data (Immunocytochemistry stain of COS7 using UCHL1 antibody (1:300).)
Testing Data #2 (Western blot detection of UCHL1 in U251,Raji&IMP32 cell lysates and using UCHL1 antibody (1:1000 diluted). Predicted band size: 25KDa Observed band size:25KDa.)
Testing Data (Immunocytochemistry stain of Hela using AKR1B1 antibody (1:300).)
Testing Data #2 (Western blot detection of AKR1B1 in HT1080, MCF7, A431&Hela cell lysates using AKR1B1 antibody (1:1000 diluted). Predicted band size:36KDa. Observed band size:36KDa.)
Immunofluorescence (IF) (Confocal immunofluorescence analysis of HL60 cells using CD18 antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
Western Blot (WB) (Western blot analysis of lysate from Y79 cell line,using GPR75 Antibody, was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)
Testing Data (Western blot detection of RAD9A in Hela, MCF7, 3T3, COS7&Rat testis cell lysates using RAD9A antibody (1:500 diluted).Predicted band size:43KDa,Observed band size:55KDa.)
Testing Data #2 (Immunoprecipitation analysis of Hela & 3T3 cell lysates using RAD9A antibody.)
Dot Blot (DB) (Dot blot analysis of rat TSC1 Antibody (Phospho Y297) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
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