BCR recombinant protein

BCR recombinant protein

Cat. Num. AAA515124

• Gene Names: BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
• Applications: Western Blot
• Synonyms: BCR; BCR recombinant protein; ALL; CML; PHL; BCR1; D22S11; D22S662; FLJ1645

• Host: Sf9 insect cells
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: 1-695
• Sequence Length: 6927

• Applicable Applications for BCR recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: Sf9 insect cells using baculovirus
• Source Note: Recombinant human BCR (1-695) was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for BCR recombinant protein

Recombinant human BCR (1-695) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: BCR (also known as breakpoint cluster region) encodes a phosphoprotein that has serine/threonine kinase activity (1). The human c-ABL oncogene is translocated from chromosome 9 to a region on chromosome 22 (associated with BCR) in chronic myelocytic leukemia. In classical t(9;22) translocation, as observed in chronic granulocytic leukemia (CGL), a hybrid DNA unit is produced comprising the BCR gene product plus the translocated c-ABL gene from chromosome 9. The BCR-ABL hybrid protein (p210) is formed that displays increased tyrosine kinase activity. A similar DNA rearrangement of the p210 protein is also found in cases of Philadelphia-positive acute lymphoblastic leukemia (ALL) (2).

Product Categories/Family for BCR recombinant protein

Cellular Proteins; Signaling Proteins - Cellular Proteins

References

1. Stam, K. et al: Evidence that the phl gene encodes a 160,000-dalton phosphoprotein with associated kinase activity. Mol. Cell Biol. 1987; 7(5): 1955-1960.
2. Eridani, S. et al: Molecular biology of Philadelphia chromosome in chronic granulocytic leukaemia and acute lymphoblastic leukaemia. Cytotechnology; 1: 33-36.

NCBI and Uniprot Product Information

• NCBI GI #: 82546842
• NCBI GeneID: 613
• NCBI Accession #: NP_004318.3
• NCBI GenBank Nucleotide #: NM_004327
• UniProt Accession #: P11274; P78501; Q12842; Q4LE80; Q6NZI3
• Molecular Weight: ~120 kDa
• NCBI Official Full Name: Homo sapiens breakpoint cluster region (BCR), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: breakpoint cluster region
• NCBI Official Symbol: BCR
• NCBI Official Synonym Symbols: ALL; CML; PHL; BCR1; D22S11; D22S662
• NCBI Protein Information: breakpoint cluster region protein; BCR/FGFR1 chimera protein; FGFR1/BCR chimera protein; renal carcinoma antigen NY-REN-26
• UniProt Protein Name: Breakpoint cluster region protein
• Protein Family: Bicyclomycin resistance protein
• UniProt Gene Name: BCR
• UniProt Synonym Gene Names: BCR1; D22S11
• UniProt Entry Name: BCR_HUMAN

NCBI Description

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity. Ref.13 Ref.15

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subunit structure: Homotetramer. Interacts with PDZK1. May interact with CCPG1

By similarity. Interacts with FES/FPS, ABL1, PIK3R1 and GRB2. Interacts with HCK. Ref.14 Ref.16 Ref.19 Ref.20 Ref.27

Domain: The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene. Ref.14The DH domain is involved in interaction with CCPG1

By similarity. Ref.14

Post-translational modification: Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2. Ref.16 Ref.19

Involvement in disease: Leukemia, chronic myeloid (CML) [MIM:608232]: A clonal myeloproliferative disorder of a pluripotent stem cell with a specific cytogenetic abnormality, the Philadelphia chromosome (Ph), involving myeloid, erythroid, megakaryocytic, B lymphoid, and sometimes T lymphoid cells, but not marrow fibroblasts.Note: The gene represented in this entry is involved in disease pathogenesis.A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).

Sequence similarities: Contains 1 C2 domain.Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.Contains 1 Rho-GAP domain.

Sequence caution: The sequence BAE06073.1 differs from that shown. Reason: Erroneous initiation.