Testing Data
(Western blot detection of RAD9A in Hela, MCF7, 3T3, COS7&Rat testis cell lysates using RAD9A antibody (1:500 diluted).Predicted band size:43KDa,Observed band size:55KDa.)
Mouse Rad9A Monoclonal Antibody | anti-Rad9A antibody
Rad9A Antibody
Testing Data
(Western blot detection of RAD9A in Hela, MCF7, 3T3, COS7&Rat testis cell lysates using RAD9A antibody (1:500 diluted).Predicted band size:43KDa,Observed band size:55KDa.)
Testing Data
(Western blot detection of RAD9A in Hela, MCF7, 3T3, COS7&Rat testis cell lysates using RAD9A antibody (1:500 diluted).Predicted band size:43KDa,Observed band size:55KDa.)
Testing Data #2
(Immunoprecipitation analysis of Hela & 3T3 cell lysates using RAD9A antibody.)
Testing Data #2
(Immunoprecipitation analysis of Hela & 3T3 cell lysates using RAD9A antibody.)
Entrez Summary: This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair.This protein possesses 3´ to 5´ exonuclease activity, which may contribute to its role in sensing and repairing DNA damage.It forms a checkpoint protein complex with RAD1 and HUS1.This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade.Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI Description
This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Research Articles on Rad9A
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Product Notes
The Rad9A rad9a (Catalog #AAA857777) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rad9A Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Rad9A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the Rad9A rad9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad9A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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