Testing Data
(Immunocytochemistry of HeLa cells using anti- BiP/GRP78 (C-terminus) antibody diluted 1:100.)
Mouse anti-Human, Rat BiP/GRP78 Monoclonal Antibody | anti-BiP/GRP78 antibody
BiP/GRP78 Antibody
Testing Data
(Immunocytochemistry of HeLa cells using anti- BiP/GRP78 (C-terminus) antibody diluted 1:100.)
Testing Data
(Immunocytochemistry of HeLa cells using anti- BiP/GRP78 (C-terminus) antibody diluted 1:100.)
Testing Data #2
(Western blot detection of BiP/GRP78 (C-terminus) in Hela, LNCAP,C6&MCF7 cell lysates using BiP/GRP78 (C-terminus) antibody (1:1000 diluted). Predicted band size: 72kDa Observed band size: 72kDa.)
Testing Data #2
(Western blot detection of BiP/GRP78 (C-terminus) in Hela, LNCAP,C6&MCF7 cell lysates using BiP/GRP78 (C-terminus) antibody (1:1000 diluted). Predicted band size: 72kDa Observed band size: 72kDa.)
Entrez Summary: The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
Uniprot Description
Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:2294010, PubMed:23769672, PubMed:23990668, PubMed:28332555). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, PubMed:19538957). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 ().
Research Articles on BiP/GRP78
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Product Notes
The BiP/GRP78 hspa5 (Catalog #AAA857756) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BiP/GRP78 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BiP/GRP78 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the BiP/GRP78 hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BiP/GRP78, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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