Immunofluorescence (IF)
(Confocal immunofluorescence analysis of HL60 cells using CD18 antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
Mouse CD18 Monoclonal Antibody | anti-CD18 antibody
CD18 antibody
Applications
ELISA, Immunofluorescence
Synonyms
CD18; Monoclonal Antibody; CD18 antibody; Monoclonal CD18; Anti-CD18; b2 Integrin; beta 2 Integrin; Cluster of differentiation 18; CD-18; CD 18; anti-CD18 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
10E12
Form/Format
Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
777
Applicable Applications for anti-CD18 antibody
ELISA (EIA), Immunofluorescence (IF)
Application Notes
ELISA: 1:10,000
IF: 1:200-1:1000
IF: 1:200-1:1000
Biological Significance
Integrins are receptors that mediate attachment between a cell and the tissues surrounding it, which may be other cells or the extracellular matrix (ECM). They also play a role in cell signaling and thereby regulate cellular shape, motility, and the cell cycle. Integrins work alongside other proteins such as cadherins, Immunoglobulin superfamily cell adhesion molecules, selectins and syndecans to mediate cell-cell and cell-matrix interaction and communication.
Cross-Reactivity
Human, Mouse
Immunogen
CD18 antibody was raised in Mouse using a purified recombinant fragment of CD18 expressed in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Confocal immunofluorescence analysis of HL60 cells using CD18 antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis of HL60 cells using CD18 antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
Related Product Information for anti-CD18 antibody
Mouse monoclonal CD18 antibody
Product Categories/Family for anti-CD18 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD18 protein
NCBI Official Symbol
cd18
NCBI Protein Information
CD18 protein
Protein Family
Similar Products
Product Notes
The CD18 (Catalog #AAA533526) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF). ELISA: 1:10,000 IF: 1:200-1:1000. Researchers should empirically determine the suitability of the CD18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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