Scientific Background: VLK or vertebrate lonesome kinase is a protein kinase which is first expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, and later its expression is confined to E-cadherin-negative mesenchyme. VLK regulates the rate of protein export from the Golgi and thereby plays an important role in the formation of functional stroma by mesenchymal cells (1). Targeted disruption of VLK leads to a defect in lung development and delayed ossification of endochondral bone. VLK deficient mice display neonatal lethality due to respiratory failure, with a suckling defect arising from a cleft palate. VLK is required for the appropriate timing of flat proliferative chondrocyte differentiation (2).
2. Imuta, Y. et al: Short limbs, cleft palate, and delayed formation of flat proliferative chondrocytes in mice with targeted disruption of a putative protein kinase gene, Pkdcc (AW548124). Dev Dyn. 2009 Jan;238(1):210-22.
NCBI and Uniprot Product Information
Uniprot Description
Function: Protein kinase which is required for longitudinal bone growth through regulation of chondrocyte differentiation. Involved in protein transport from the Golgi apparatus to the plasma membrane
By similarity.
Subcellular location: Golgi apparatus
By similarity.
Sequence similarities: Belongs to the protein kinase superfamily.Contains 1 protein kinase domain.
Sequence caution: The sequence AAH94697.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI10514.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI10515.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on VLK
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Product Notes
The VLK pkdcc (Catalog #AAA515268) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant human VLK (129-end) was expressed by baculovirus in Sf9 insect cells. AAA Biotech's VLK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the VLK pkdcc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VLK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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