Rabbit anti-Human Bassoon Polyclonal Antibody | anti-BSN antibody

Bassoon Antibody: Alkaline Phosphatase

Cat. Num. AAA805829

• Gene Names: BSN; ZNF231
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, ELISA, Dot Blot
• Synonyms: Bassoon; Polyclonal Antibody; Bassoon Antibody: Alkaline Phosphatase; OC Antibody; Fibrils Antibody; Amyloid Oligomer ars Antibody; All Antibody; Amyloid beta A4 protein Antibody; ABPP Antibody; APPI Antibody; Alzheimer disease amyloid protein Antibody; Cerebral vascular amyioid peptide Antibody; PreA4 AntiDody; Protease nexin-II Antibody; APP Antibody; A4 Antibody; AD Antibody; anti-BSN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrililar oligomers or natively folded proteins. Expected to detect in Mouse and Rat based on species homology.
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 3926

• Applicable Applications for anti-BSN antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry(ICC), Immunofluoresence (IF), Immunoprecipitation (IP), ELISA, Dot Blot (DB)
• Application Notes: DB (l:1000); Optimal dilutions for assays should be determined by the user.
• Conjugate: Alkaline Phosphatase
• Immunogen: Fibrils prepared from human ABeta42 peptide
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Certificate of Analysis: A 1:1000 dilution of MBS805829 was sufficient for detection of amyloid fibrils on PVDF membranes using transferred fibrils by colorimetric dot blot analysis using Goat anti-rabbit IgG: HRP as the secondary antibody.
• Research: Neuroscience; Cell Signaling; Cardiovascular System; Blood
• Cellular Localization: Membrane
• Preparation and Storage: -20 degree C. Avoid freeze thaw cycle.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.)

Western Blot (WB)

(Western blot analysis of Mouse, Rat brain cell lysates showing detection of Bassoon protein using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:1000.)

Related Product Information for anti-BSN antibody

Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres (1, 2). These include the amyloid-~ peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of a-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease (2, 3).

Product Categories/Family for anti-BSN antibody

Neuroscience

References

1. Anna Dondzillo et al., (2010) J. Comp Neur, 518(7): 1008-1029.

NCBI and Uniprot Product Information

• NCBI GI #: 91208420
• NCBI GeneID: 8927
• NCBI Accession #: NP_003449.2
• NCBI GenBank Nucleotide #: NM_003458.3
• UniProt Accession #: Q9UPA5; O43161; Q7LGH3
• Molecular Weight: 416,469 Da
• NCBI Official Full Name: protein bassoon
• NCBI Official Synonym Full Names: bassoon presynaptic cytomatrix protein
• NCBI Official Symbol: BSN
• NCBI Official Synonym Symbols: ZNF231
• NCBI Protein Information: protein bassoon; zinc finger protein 231; neuronal double zinc finger protein; bassoon (presynaptic cytomatrix protein)
• UniProt Protein Name: Protein bassoon
• Protein Family: Protein bassoon
• UniProt Gene Name: BSN
• UniProt Synonym Gene Names: KIAA0434; ZNF231
• UniProt Entry Name: BSN_HUMAN

NCBI Description

Neurotransmitters are released from a specific site in the axon terminal called the active zone, which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures, and is essential for regulating neurotransmitter release from a subset of synapses. [provided by RefSeq, Jul 2008]

Uniprot Description

BSN: involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses. Interacts with CAST1, RIMS1 and UNC13A. Part of a complex consisting of ERC2, RIMS1 and BSN. Localized to the active zone of presynaptic density. Mice lacking functional BSN showed a reduced excitability attributed to inactivation of a fraction of brain glutamatergic synapses. At these synapses, vesicles are clustered and docked in normal numbers, but are unable to fuse. In retina, mutants lacking functional BSN showed normal retinal anatomy, but synapses lacked anchoring of the photoreceptor ribbon to the presynaptic active zone resulting in impaired photoreceptor synaptic transmission. Two alternatively spliced isoforms have been reported.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: axon; dendrite; postsynaptic density; presynaptic active zone; excitatory synapse; cell junction; nucleus

Molecular Function: metal ion binding

Biological Process: synaptic transmission