SDS-PAGE
PI3K (p55 gamma) recombinant protein
PI3K (p55 gamma) recombinant protein
Gene Names
PIK3R3; p55; p55-GAMMA
Applications
Western Blot
Synonyms
PI3K (p55 gamma); PI3K (p55 gamma) recombinant protein; PIK3R3; p55; p55-GAMMA; FLJ41892; DKFZp686P05226; PI3K (p55gamma) Protein; PI3K (p55g) Protein
Host
Insect cells
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Sequence Length
5621
Applicable Applications for PI3K (p55 gamma) recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full length human PI3K (p55 gamma) was expressed in insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for PI3K (p55 gamma) recombinant protein
Recombinant full length human PI3K (p55 gamma) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Scientific Background: PI3K (p55 gamma) also known as PI3K regulatory subunit 3 (PIK3R3) was first identified by 2-hybrid system as a protein that bind to the intracellular domain of IGF1R. PIK3R3 contains 2 Src homology-2 (SH2) domains, an inter-SH2/p110-binding site and a proline-rich motif. PIK3R3 transcript is detected in all fetal and adult tissues except peripheral blood leukocytes. Expression is low in adult liver and most abundant in adult testis. PIK3R3 can bind IGF1R, INSR, and INSR substrate-1 in vitro and the binding requires receptor activation (1).
Scientific Background: PI3K (p55 gamma) also known as PI3K regulatory subunit 3 (PIK3R3) was first identified by 2-hybrid system as a protein that bind to the intracellular domain of IGF1R. PIK3R3 contains 2 Src homology-2 (SH2) domains, an inter-SH2/p110-binding site and a proline-rich motif. PIK3R3 transcript is detected in all fetal and adult tissues except peripheral blood leukocytes. Expression is low in adult liver and most abundant in adult testis. PIK3R3 can bind IGF1R, INSR, and INSR substrate-1 in vitro and the binding requires receptor activation (1).
Product Categories/Family for PI3K (p55 gamma) recombinant protein
References
1. Dey, B R. et al: Cloning of human p55-gamma, a regulatory subunit of phosphatidylinositol 3-kinase, by a yeast two-hybrid library screen with the insulin-like growth factor-I receptor. Gene 209: 175-183, 1998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~61 kDa
NCBI Official Full Name
Homo sapiens phosphoinositide-3-kinase, regulatory subunit 3 (gamma), mRNA
NCBI Official Synonym Full Names
phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
NCBI Official Symbol
PIK3R3
NCBI Official Synonym Symbols
p55; p55-GAMMA
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit gamma; p55PIK; PI3-kinase subunit p55-gamma; PI3K regulatory subunit gamma; 100% homology to SWISS-PROT Q92569; PI3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma); phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphoinositide-3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma)
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit gamma
UniProt Gene Name
PIK3R3
UniProt Synonym Gene Names
PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma
UniProt Entry Name
P55G_HUMAN
Uniprot Description
Function: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
Subunit structure: Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Ref.6
Tissue specificity: Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
Sequence similarities: Belongs to the PI3K p85 subunit family.Contains 2 SH2 domains.
Research Articles on PI3K (p55 gamma)
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Product Notes
The PI3K (p55 gamma) pik3r3 (Catalog #AAA515204) is a Recombinant Protein produced from Insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full length human PI3K (p55 gamma) was expressed in insect cells. AAA Biotech's PI3K (p55 gamma) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PI3K (p55 gamma) pik3r3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3K (p55 gamma), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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