SDS-Page
ECD recombinant protein
ECD Protein, Human, Recombinant (His & GST Tag)
Gene Names
ECD; GCR2; HSGT1
Purity
>90% as determined by SDS-PAGE
Synonyms
ECD; ECD Protein; Human; Recombinant (His & GST Tag); Human ECD/Ecdysoneless homolog Protein (His & GST Tag); GCR2 Protein; HSGT1 Protein; ecdysoneless cell cycle regulator; ECD recombinant protein
Host
Baculovirus-Insect Cells
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Thr621
Species
Human
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Met
Tag
N-GST & His
Protein Construction
A DNA sequence encoding the human ECD (O95905-1)(Met1-Thr621) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for ECD recombinant protein
Background: ECD, also known as ecdysoneless homolog, belongs to the SGT1 family. It is highly expressed in muscle and heart. ECD is a novel promoter of mammalian cell cycle progression. This function is related to its ability to remove the repressive effects of Rb-family tumor suppressors on E2F transcription factors. It is a novel tumor-promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells. ECD may also be a transcriptional activator required for the expression of glycolytic genes.
References
Badzek S. et al., 2011, Wien Klin Wochenschr. 123 (23-24): 726-31.Zhao X. et al., 2012, Breast Cancer Res Treat. 134 (1): 171-80.Dey P. et al., 2012, Clin Cancer Res. 18 (22): 6188-98.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
644
NCBI Official Full Name
Protein SGT1
NCBI Official Synonym Full Names
ecdysoneless homolog (Drosophila)
NCBI Official Symbol
ECD
NCBI Official Synonym Symbols
GCR2; HSGT1
NCBI Protein Information
protein SGT1; suppressor of GCR2; protein ecdysoneless homolog; suppressor of S. cerevisiae gcr2
UniProt Protein Name
Protein SGT1
Protein Family
UniProt Gene Name
ECD
UniProt Synonym Gene Names
SGT1; hSGT1
UniProt Entry Name
SGT1_HUMAN
Uniprot Description
SGT1: Novel regulator of p53 stability and function. May also be a transcriptional activator required for the expression of glycolytic genes. Belongs to the SGT1 family.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 10q22.3
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein binding; transcription coactivator activity
Biological Process: transcription from RNA polymerase II promoter; cell proliferation; regulation of transcription, DNA-dependent; regulation of glycolysis
Research Articles on ECD
Similar Products
Product Notes
The ECD ecd (Catalog #AAA8121666) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Thr62 1. It is sometimes possible for the material contained within the vial of "ECD, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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