Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Plasma Cell Marker Monoclonal Antibody (SPM310).)
Mouse Plasma Cell Marker Monoclonal Antibody
Plasma Cell Marker Mouse Monoclonal Antibody
Reactivity
Human.Does not react with Rat
Applications
Immunohistochemistry
Synonyms
Plasma Cell Marker; Monoclonal Antibody; Plasma Cell Marker Mouse Monoclonal Antibody; Plasma B cell; Plasmocytes; anti-Plasma Cell Marker antibody
Host
Mouse
Reactivity
Human.
Does not react with Rat
Does not react with Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
SPM310
Specificity
It recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4+ lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm; their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This MAb superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma. Note that this MAb is not suitable for staining frozen tissues.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-Plasma Cell Marker antibody
Immunohistochemistry (IHC) Formalin
Application Notes
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Cellular Localization
Cytoplasmic
Immunogen
Pancreatic cancer-related mucin
Positive Control
Tonsil or lymph node
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Plasma Cell Marker Monoclonal Antibody (SPM310).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Plasma Cell Marker Monoclonal Antibody (SPM310).)
References
Turley H et. al. Journal of Clinical Pathology, 1994, 47(5):418-22
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Product Notes
The Plasma Cell Marker (Catalog #AAA438283) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Plasma Cell Marker Mouse Monoclonal Antibody reacts with Human. Does not react with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Plasma Cell Marker can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin. Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the Plasma Cell Marker for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Plasma Cell Marker, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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