Pig interleukin 4 ELISA Kit | IL4 elisa kit

Pig interleukin 4, IL-4 ELISA Kit

Cat. Num. AAA702795

• Reactivity: Pig
• Synonyms: interleukin 4; Pig interleukin 4; IL-4 ELISA Kit; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; IL4 elisa kit

• Reactivity: Pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pig IL4. No significant cross-reactivity or interference between Pig IL4 and analogues was observed.
• Sequence Length: 133

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 6.25 pg/ml-400 pg/ml
• Sensitivity: 1.56 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 3: B-cell activation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL4 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for IL-4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55742622
• NCBI GeneID: 397225
• NCBI Accession #: NP_999288.1
• NCBI GenBank Nucleotide #: NM_214123.1
• UniProt Accession #: Q04745; Q29054; Q8MKE6
• Molecular Weight: 15,023 Da
• NCBI Official Full Name: interleukin-4
• NCBI Official Symbol: IL4
• NCBI Protein Information: interleukin-4; IL-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: IL4
• UniProt Synonym Gene Names: IL-4; BSF-1
• UniProt Entry Name: IL4_PIG

Uniprot Description

Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.