Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for " Primary Antibodies A" - showing 350-400
Western Blot (WB)
(Western blot analysis of CCL2 Antibody (C-term) in Hela cell line lysates (35ug/lane). CCL2 (arrow) was detected using the purified Pab.)
CCL2, Polyclonal Antibody (Cat# AAA9207814)
Full Name
CCL2 Antibody (C-term)
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PSD2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
PSD2, Polyclonal Antibody (Cat# AAA9134432)
Full Name
PSD2 Polyclonal Antibody
Gene Names
PSD2; EFA6C
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of anti-CSF1R Pab in human placenta. CSF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA9211301)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Anti-CASK (Center K227) Antibody at 1:1000 dilution + SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
CASK, Polyclonal Antibody (Cat# AAA9217136)
Full Name
CASK Antibody (Center K227)
Gene Names
CASK; CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA
Reactivity
Human; Predicted Reactivity: Mouse, Rat
Applications
WB, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot of ACVRL1 Pab. TOP LEFT: Mouse heart tissue lysate.)
ACVRL1, Polyclonal Antibody (Cat# AAA9208190)
Full Name
ACVRL1 Antibody (N-term)
Gene Names
ACVRL1; HHT; ALK1; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1
Reactivity
Human, mouse
Applications
WB, EIA, FC/FACS, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Staining of rat peritoneal macrophages with Mouse anti Rat CD172a:RPE (MBS213862))
CD172a, Monoclonal Antibody (Cat# AAA211068)
Full Name
MOUSE ANTI RAT CD172a:FITC
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS
Pricing
$425/0.1 mg | $1,755/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Hela whole cell lysates, using SLC3A2 Antibody. The lane on the left is treated with the antigen-specific peptide.)
SLC3A2, Polyclonal Antibody (Cat# AAA9608104)
Full Name
SLC3A2 Antibody
Gene Names
SLC3A2; 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
Reactivity
Human
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of anti-PI3KCA Pab in HeLa cell lysate. PI3KCA (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
PI3KCA, Polyclonal Antibody (Cat# AAA9200737)
Full Name
PI3KCA Antibody (C-term)
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, FC, IHC-p
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC)
(Anti-STX5 / Syntaxin 5 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)
STX5 / Syntaxin 5, Polyclonal Antibody (Cat# AAA2400126)
Full Name
Anti-STX5 / Syntaxin 5 Antibody (aa51-307) IHC-plus
Gene Names
STX5; SED5; STX5A
Reactivity
Rat, Human
Applications
IHC, ICC, WB
Purity
Immunoaffinity purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Western Blot (WB)
Phospho-PDE4A, Polyclonal Antibody (Cat# AAA540102)
Full Name
Phospho-PDE4A Antibody
Reactivity
Human, Mouse, Rabbit, Rat
Applications
EIA, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from HepG2, K562, NCI-H460 cell line (from left to right), using ALDH1A1 Antibody. MBS9200073 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
ALDH1A1, Monoclonal Antibody (Cat# AAA9200073)
Full Name
ALDH1A1 Antibody
Gene Names
ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
Reactivity
Human
Applications
WB, EIA, IF, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005611)
Full Name
VPS35 Antibody, Clone 7E4: RPE
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$535/0.1 mg | $2,175/5x0.1 mg
Immunohistochemistry (IHC)
(Anti-HSP90AA / HSP90 Alpha antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
HSP90AA / HSP90 Alpha, Monoclonal Antibody (Cat# AAA245831)
Full Name
Mouse Monoclonal [clone 2G5.G3] (IgG) to Human HSP90AA / HSP90 Alpha
Reactivity
Human, Mouse, Rat
Applications
IHC - Paraffin, ICC, WB, IP, EIA
Purity
Protein G Purified from tissue culture supernatant
Pricing
$585/0.05 mg | $2,585/5x0.05 mg
Immunohistochemistry (IHC)
(S-100 protein expression in the cutaneous malignant melanoma. Formalin fixed, paraffin embedded human tissue (4 um section) stained with anti-S-100 (MBS684077) monospecific clonal antibody according to related datasheet.)
S-100, Monoclonal Antibody (Cat# AAA684077)
Full Name
Anti - S-100
Gene Names
S100A1; S100; S100A; S100-alpha
Applications
IHC-P
Pricing
$380/7 mL (RTU) | $360/0.1 mL (Concentrate) | $585/0.5 mL (Concentrate) | $890/1 mL (Concentrate) | $470/0.2 mL (Concentrate) | $550/15 mL (RTU) | $195/1 mL (RTU) | $205/0.04 mL (Concentrate) | $3,745/5x1 mL (Concentrate)
Immunohistochemistry - Paraffin (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
PARPBP / C12orf48, Polyclonal Antibody (Cat# AAA249625)
Full Name
Anti-PARPBP / C12orf48 Antibody (aa522-550) IHC-plus
Gene Names
PARPBP; AROM; PARI; C12orf48
Reactivity
Human
Applications
IHC, WB, FC/FACS
Purity
Protein A purified
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody (C-term). MBS9203824 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
SIRT3, Polyclonal Antibody (Cat# AAA9203824)
Full Name
SIRT3 Antibody (C-term)
Gene Names
SIRT3; SIR2L3
Reactivity
Human, mouse
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from A431, Hela cell line (from left to right), using SEPT9 Antibody (C-term). MBS9215150 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
SEPT9, Polyclonal Antibody (Cat# AAA9215150)
Full Name
SEPT9 Antibody (C-term)
Gene Names
SEPT9; MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells stained with SMAD2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9204752 SMAD2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.)
SMAD2, Polyclonal Antibody (Cat# AAA9204752)
Full Name
SMAD2 Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, mouse (Predicted Reactivity: Drosophila, Bovine, Zebrafish, Rat, Chicken, Pig)
Applications
IF, EIA, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
((0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Calnexin, Polyclonal Antibody (Cat# AAA422230)
Full Name
Goat anti-Calnexin Antibody
Gene Names
CANX; CNX; P90; IP90
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
EIA, WB, IHC, EIA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808918)
Full Name
Alpha Synuclein Antibody, Clone 3C11: ATTO 488
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human; Mouse; Rat
Applications
WB, DB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunofluorescence (IF)
(Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) (C-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9212277 SYVN1 (HRD1) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).)
SYVN1 (HRD1), Polyclonal Antibody (Cat# AAA9212277)
Full Name
SYVN1 (HRD1) Antibody (C-term)
Gene Names
SYVN1; DER3; HRD1
Reactivity
Human, mouse
Applications
IF, EIA, IHC, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilutionLane 1: human brain lysateLane 2: mouse liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
GLS2, Monoclonal Antibody (Cat# AAA9230948)
Full Name
GLS2 Antibody
Gene Names
GLS2; GA; GLS; LGA; hLGA
Reactivity
Human, Mouse
Applications
WB, EIA, IHC-P
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB)
( All lanes : Anti-RAF1 Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
RAF1, Polyclonal Antibody (Cat# AAA9217155)
Full Name
RAF1 Antibody (S233)
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human
Applications
WB, IHC-P, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of anti-ABL Pab in mouse colon tissue lysate (35ug/lane). ABL(arrow) was detected using the purified Pab.)
Phospho-ABL1 (Y245), Polyclonal Antibody (Cat# AAA9214737)
Full Name
Phospho-ABL1 (Y245) Antibody
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
Reactivity
Human, mouse
Applications
WB, EIA, IHC, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808823)
Full Name
Methylglyoxal Antibody, Clone 9F11: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005673)
Full Name
VPS35 Antibody, Clone 10A8: Biotin
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$535/0.1 mg | $2,170/5x0.1 mg
Western Blot (WB)
(Anti-RAF1 Antibody at 1:1000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
RAF1, Polyclonal Antibody (Cat# AAA9217154)
Full Name
RAF1 Antibody (S621)
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human; Predicted Reactivity: Bovine, Chicken, Mouse, Rat
Applications
WB, IHC, EIA
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808931)
Full Name
Alpha Synuclein Antibody, Clone 3C11: PerCP
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human; Mouse; Rat
Applications
WB, DB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with GABARAP (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9204011 GABARAP (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
GABARAP, Polyclonal Antibody (Cat# AAA9204011)
Full Name
GABARAP Antibody (N-term)
Gene Names
GABARAP; MM46; ATG8A; GABARAP-a
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rabbit, Rat)
Applications
IF, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Published customer image: Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. All proteins were immunolocalised to cell nuclei (see higher power inserts in panels e, f, c and d respectively). In the well and moderately differentiated cancers expression was most intense in epithelial cell layers (arrowheads, panel A and inserts). Note that ERa was low/absent in poor grade cancers (J) but immunoexpression of ERbeta1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERbeta1, ERbeta2 and ERbeta5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers.From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330.)
ESTROGEN RECEPTOR BETA 2, Monoclonal Antibody (Cat# AAA219399)
Full Name
MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 2
Gene Names
ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
Applications
Immunohistology Paraffin*
Pricing
$905/0.1 mg | $3,910/5x0.1 mg
Western Blot (WB)
((0. 01ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Fumarase/FH, Polyclonal Antibody (Cat# AAA421119)
Full Name
Goat anti-Fumarase/FH Antibody
Gene Names
FH; MCL; FMRD; LRCC; HLRCC; MCUL1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
EIA, WB, EIA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from 293, T47D cell line (from left to right), using APG8c (MAP1LC3C) Antibody ?M1?. MBS9212772 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
LC3, Polyclonal Antibody (Cat# AAA9212772)
Full Name
LC3 Antibody (APG8C) (N-term)
Gene Names
MAP1LC3C; LC3C; ATG8J
Reactivity
Human
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Dot Blot (DB)
(Dot blot analysis of anti-hp53-T18 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.)
Phospho-p53 (T18), Polyclonal Antibody (Cat# AAA9204992)
Full Name
Phospho-p53 (T18) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, mouse (Predicted Reactivity: Monkey, Pig, Rabbit)
Applications
DB, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)
Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000267)
Full Name
Cyclooxygenase 1 Antibody: ATTO 488
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunofluorescence (IF)
(Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9200291 MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976).)
MET/HGFR, Monoclonal Antibody (Cat# AAA9200291)
Full Name
MET/HGFR Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human, mouse
Applications
IF, EIA, IHC, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Anti-FAT4 Antibody at 1:2000 dilution + Recombinant proteinLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 543 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
FAT4, Monoclonal Antibody (Cat# AAA9217029)
Full Name
FAT4 Antibody
Gene Names
FAT4; FATJ; FAT-J; CDHF14; CDHR11; HKLLS2; VMLDS2; NBLA00548
Reactivity
Human
Applications
WB, EIA
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-PCSK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
PCSK1 / PC1, Monoclonal Antibody (Cat# AAA246212)
Full Name
Mouse Monoclonal [clone 3D2] (IgG3,k) to Human PCSK1 / PC1
Gene Names
PCSK1; PC1; PC3; NEC1; SPC3; BMIQ12
Reactivity
Human, Mouse, Rat
Applications
IHC - Paraffin, IF, WB, EIA
Purity
Purified fromascites by Protein A
Pricing
$650/0.05 mg | $2,875/5x0.05 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6. Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)
KCNQ4, Monoclonal Antibody (Cat# AAA800140)
Full Name
KCNQ4 Antibody: Alkaline Phosphatase
Gene Names
KCNQ4; DFNA2; KV7.4; DFNA2A
Reactivity
Human, Mouse, Rat
Applications
WB, IP, ICC, IF
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse CD99 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Cluster Of Differentiation 99, Polyclonal Antibody (Cat# AAA2032865)
Full Name
Cluster Of Differentiation 99 (CD99) Polyclonal Antibody
Gene Names
Cd99; D4; Pilr-l; pilr-1; 1110061M03Rik; 2410026K10Rik
Reactivity
Mouse
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$160/0.1 mL | $205/0.2 mL | $340/0.5 mL | $415/1 mL | $1,205/5x1 mL
Immunofluorescence (IF)
(Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9200115 MET/HGFR primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976).)
MET/HGFR, Monoclonal Antibody (Cat# AAA9200115)
Full Name
MET/HGFR Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human, mouse
Applications
IF, EIA, IHC, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36. Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.. Courtesy of: Francesco Paonessa.)
Tau, Monoclonal Antibody (Cat# AAA8005587)
Full Name
Tau Antibody (pSer202/ pThr205), Clone AH36: RPE
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse
Applications
WB, DB, ICC, IF, EIA, IHC
Purity
Affinity Purified
Pricing
$620/0.1 mg | $2,570/5x0.1 mg
Western Blot (WB)
(Western blot analysis of anti-CCBP2 Pab in mouse small intestine tissue lysate. CCBP2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Chemokine Receptor D6 (CCBP2), Polyclonal Antibody (Cat# AAA9213300)
Full Name
Chemokine Receptor D6 (CCBP2) Antibody (C-term)
Gene Names
ACKR2; D6; hD6; CCR9; CCBP2; CCR10; CMKBR9
Reactivity
Human, mouse
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Anti-ADAMTS17 Antibody (N-term) at 1:2000 dilution + A2058 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 121 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
ADAMTS17, Polyclonal Antibody (Cat# AAA9217219)
Full Name
ADAMTS17 Antibody (N-term)
Reactivity
Human
Applications
WB, EIA
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dibromo-tyrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dibromo-tyrosine, Monoclonal Antibody (Cat# AAA808614)
Full Name
Dibromo-tyrosine Antibody, Clone 6G3: ATTO 594
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Western Blot (WB)
(SUMO2/3 Antibody (C-term E69) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).)
SUMO2/3, Polyclonal Antibody (Cat# AAA9212829)
Full Name
SUMO2/3 Antibody (C-term E69)
Gene Names
SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
Reactivity
Human (Predicted Reactivity: Xenopus, Zebrafish, Bovine, Chicken, Hamster, Monkey, Mouse, Pig, Rat)
Applications
EIA, IHC, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808574)
Full Name
Acrolein Antibody, Clone 2H2
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808650)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5D9: ATTO 594
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Testing Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647 (MBS215657A647))
CD206, Monoclonal Antibody (Cat# AAA215662)
Full Name
RAT ANTI MOUSE CD206:FITC
Gene Names
Mrc1; MR; CD206; AW259686
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rab5 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). RAB5 (arrow) was detected using the purified Pab.)
Rab5, Polyclonal Antibody (Cat# AAA9206094)
Full Name
Rab5 Antibody (C-term)
Gene Names
RAB5A; RAB5
Reactivity
Human
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6. Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)
KCNQ4, Monoclonal Antibody (Cat# AAA802222)
Full Name
KCNQ4 Antibody: PerCP
Gene Names
KCNQ4; DFNA2; KV7.4; DFNA2A
Reactivity
Human, Mouse, Rat
Applications
WB, IP, ICC, IF
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
