Rabbit anti-Human SEPT9 Polyclonal Antibody | anti-SEPT9 antibody

SEPT9 Antibody (C-term)

Cat. Num. AAA9215150

• Gene Names: SEPT9; MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SEPT9; Polyclonal Antibody; SEPT9 Antibody (C-term); Septin-9; MLL septin-like fusion protein MSF-A; MLL septin-like fusion protein; Ovarian/Breast septin; Ov/Br septin; Septin D1; KIAA0991; MSF; anti-SEPT9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SEPT9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-85 amino acids from the C-terminal region of human SEPT9.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 557-586
• Sequence Length: 362

• Applicable Applications for anti-SEPT9 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from A431, Hela cell line (from left to right), using SEPT9 Antibody (C-term). MBS9215150 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB)

(Western blot analysis of lysates from human kidney and liver tissue lysate (from left to right), using SEPT9 Antibody (C-term). MBS9215150 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(The anti-SEPT9 Pab is used in Western blot to detect SEPT9 in Jurkat cell lysate.)

Immunohistochemistry (IHC)

(SEPT9 Antibody (A555) immunohistochemistry analysis in formalin fixed and paraffin embedded kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEPT9 Antibody (A555) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SEPT9 Antibody (A555) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SEPT9 antibody

The maf oncogene was identified by structural analysis of the AS42 avian transforming retrovirus genome. The Maf family is divided into two subclasses, large Mafs (vMaf, cMaf, MafB and Nrl) and small Mafs (MafF, MafK, and MafG). Both subclasses contain leucinezipper motifs, which allow homodimerization as well as heterodimerization with a variety of other bZip transcription factors. Large Mafs also contain an acidic transactivation domain absent in the small Maf proteins. Although they do not possess inherent transactivation activity, small Maf proteins can act as positive regulators of transcription by targeting transcriptionally active dimerization partners to specific DNA regulatory elements. Conversely, small Mafs can act also as negative regulators of transcription by recruiting transcriptional repressors or by forming homodimers that can replace active dimers. Human MafF was isolated in a yeast one-hybrid system from a human myometrium cDNA library. Human MAFF encodes a 164 amino acids proten. Like other small MAFF proteins, it contains an extended leucine zipper structure and lacks an N-terminal transactivating domain. The three small Maf proteins have been implicated in a number of physiological processes, including development, differentiation, haematopoiesis and stress response. Interestingly, these three proteins regulate the stress response via different mechanisms.

Product Categories/Family for anti-SEPT9 antibody

Crown Antibodies; Cell Biology

References

Proc. Natl. Acad. Sci. U.S.A. 96:6428-6433(1999). Cancer Res. 60: 4729-4734, 2000. Oncogene 20: 5930-5939, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 164698494
• NCBI GeneID: 10801
• NCBI Accession #: NP_001106963.1; NP_001106964.1; NP_001106965.1; NP_001106966.1; NP_001106967.1; NP_001106968.1; NP_006631.2
• NCBI GenBank Nucleotide #: NM_001113491.1
• UniProt Accession #: Q9UHD8; Q96QF3; Q96QF4; Q96QF5; Q9HA04; Q9UG40; A8K2V3; B3KPM0; B4DTL9; B4E0N2; B4E274; B7Z654
• Molecular Weight: 65401
• NCBI Official Full Name: septin-9 isoform a
• NCBI Official Synonym Full Names: septin 9
• NCBI Official Symbol: SEPT9
• NCBI Official Synonym Symbols: MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25
• NCBI Protein Information: septin-9
• UniProt Protein Name: Septin-9
• UniProt Gene Name: SEPT9
• UniProt Synonym Gene Names: KIAA0991; MSF; MLL septin-like fusion protein; Ov/Br septin
• UniProt Entry Name: SEPT9_HUMAN

NCBI Description

This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2009]

Uniprot Description

SEPT9: a member of a family of novel GTP binding proteins that have been implicated in cytokinesis and exocytosis. This family include the cell division control proteins CDC3, CDC10, CDC11. Required for the completion of cytokinesis and suggest a role that is distinct from that of Nedd5.

Protein type: Cell cycle regulation; Cytoskeletal; Hydrolase

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: microtubule; perinuclear region of cytoplasm; cytoplasm; stress fiber; actin cytoskeleton

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: protein heterooligomerization; cell division; metabolic process; cell cycle

Disease: Amyotrophy, Hereditary Neuralgic