Rabbit anti-Rat, Human STX5 / Syntaxin 5 Polyclonal Antibody | anti-STX5 antibody

Anti-STX5 / Syntaxin 5 Antibody (aa51-307) IHC-plus

Cat. Num. AAA2400126

• Gene Names: STX5; SED5; STX5A
• Reactivity: Rat, Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: STX5 / Syntaxin 5; Polyclonal Antibody; Anti-STX5 / Syntaxin 5 Antibody (aa51-307) IHC-plus; Rabbit Polyclonal (IgG) to Human STX5 / Syntaxin 5; Human STX5 / Syntaxin 5; SED5; STX5A; Syntaxin 5A; Syntaxin-5; Syntaxin 5; anti-STX5 antibody

• Host: Rabbit
• Reactivity: Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human STX5 / Syntaxin 5
• Purity/Purification: Immunoaffinity purified
• Form/Format: 0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
• Concentration: 1.01 mg/ml (varies by lot)
• Sequence Length: 355

• Applicable Applications for anti-STX5 antibody: Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC (1:100 - 1:1000); IHC-P (7.5 ug/ml); WB (1:1000 - 1:10000)
• Immunogen: STX5 / Syntaxin 5 antibody was raised against recombinant fragment corresponding to a region within amino acids 51 and 307 of Syntaxin 5 (SwissProt Q13190).
• Immunogen Description: Recombinant fragment corresponding to a region within amino acids 51 and 307 of Syntaxin 5 (SwissProt Q13190).
• Immunogen Type: Recombinant protein
• Antigen Modification: aa51-307
• Target Species: Human
• Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Keep as concentrated solution. For short term storage store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-STX5 / Syntaxin 5 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)

Immunocytochemistry (ICC)

(Immunofluorescence of methanol-fixed A431 using Syntaxin 5 antibody at 1:200 dilution.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A: A431. 12% SDS PAGE. STX5 / Syntaxin 5 antibody diluted at 1:5000.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A: PC-12. 12% SDS PAGE. STX5 / Syntaxin 5 antibody diluted at 1:5000.)

Related Product Information for anti-STX5 antibody

STX5 Antibody, SED5 Antibody, STX5A Antibody, Syntaxin 5A Antibody, Syntaxin-5 Antibody, Syntaxin 5 Antibody Description: STX5 / Syntaxin 5 is a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for v-SNAREs (vesicle-SNAP receptors), permitting specific synaptic vesicle docking and fusion. The encoded protein regulates endoplasmic reticulum to Golgi transport and plays a critical role in autophagy. Autoantibodies targeting the encoded protein may be a marker for endometriosis.

NCBI and Uniprot Product Information

• NCBI GI #: 94400932
• NCBI GeneID: 6811
• NCBI Accession #: NP_003155.2
• NCBI GenBank Nucleotide #: NM_003164.4
• UniProt Accession #: Q13190; Q5U0D4; Q7Z3T6; Q9BUG1; B2R8T2; F8W8Q9
• NCBI Official Full Name: syntaxin-5 isoform 1
• NCBI Official Synonym Full Names: syntaxin 5
• NCBI Official Symbol: STX5
• NCBI Official Synonym Symbols: SED5; STX5A
• NCBI Protein Information: syntaxin-5
• UniProt Protein Name: Syntaxin-5
• Protein Family: Syntaxin
• UniProt Gene Name: STX5
• UniProt Synonym Gene Names: STX5A
• UniProt Entry Name: STX5_HUMAN

NCBI Description

This gene encodes a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for v-SNAREs (vesicle-SNAP receptors), permitting specific synaptic vesicle docking and fusion. The encoded protein regulates endoplasmic reticulum to Golgi transport and plays a critical role in autophagy. Autoantibodies targeting the encoded protein may be a diagnostic marker for endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

STX5: Mediates endoplasmic reticulum to Golgi transport. Belongs to the syntaxin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; Golgi apparatus; Golgi membrane; integral to membrane; nucleoplasm; SNARE complex; vesicle

Molecular Function: protein binding; protein N-terminus binding; SNAP receptor activity; SNARE binding

Biological Process: cellular protein metabolic process; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; intracellular protein transport; positive regulation of protein catabolic process; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; retrograde transport, endosome to Golgi; vesicle docking; vesicle fusion; vesicle fusion with Golgi apparatus