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10000 results for " Primary Antibodies A" - showing 400-450


Flow Cytometry (FC/FACS) (FLT3 (CD135) Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

FLT3 (CD135), Polyclonal Antibody (Cat# AAA9214434)

Full Name
FLT3 (CD135) Antibody (N-term)
Gene Names
FLT3; FLK2; STK1; CD135; FLK-2
Reactivity
Human
Applications
FC/FACS, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Anti-AMPK2 Antibody (Center) at 1:1000 dilution + 293 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

AMPK2, Polyclonal Antibody (Cat# AAA9217141)

Full Name
AMPK2 Antibody (Center)
Gene Names
PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2
Reactivity
Human; Predicted Reactivity: Mouse, Pig, Rat
Applications
WB, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of MCF-7 cell stained with TREF1 Antibody (C-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TREF1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).TREF1 immunoreactivity is localized to Nucleus significantly.)

TREF1, Polyclonal Antibody (Cat# AAA9200686)

Full Name
TREF1 Antibody (C-term)
Gene Names
TRERF1; RAPA; BCAR2; TREP132; TReP-132; HSA277276; dJ139D8.5
Reactivity
Human
Applications
FC/FACS, IF, WB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Staining (0.3ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

CMG1/CCDC2/IFT74, Polyclonal Antibody (Cat# AAA421519)

Full Name
Goat anti-CMG1/CCDC2/IFT74 Antibody
Gene Names
IFT74; CMG1; CCDC2; CMG-1
Reactivity
Tested: Human, Rat; Expected from sequence similarity: Human, Rat
Applications
EIA, WB, IHC, IF
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB) (OAT Antibody (N-term) western blot analysis in 293,293T cell line,mouse liver and rat liver tissue lysates (35ug/lane).This demonstrates the OAT antibody detected the OAT protein (arrow).)

OAT, Polyclonal Antibody (Cat# AAA9207986)

Full Name
OAT Antibody (N-term)
Gene Names
OAT; OKT; GACR; HOGA; OATASE
Reactivity
Human, mouse, rat
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (NPM1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NPM1 antibody detected the NPM1 protein (arrow).)

NPM1, Polyclonal Antibody (Cat# AAA9202409)

Full Name
NPM1 Antibody (N-term)
Gene Names
NPM1; B23; NPM
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunofluorescence (IF) (Fluorescent confocal image of HeLa cells stained with phospho-Bad-S99 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9207687 phospho-Bad-S99 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-Bad-S99 mainly to the cytoplasm.)

Phospho-Bad (S99), Polyclonal Antibody (Cat# AAA9207687)

Full Name
Phospho-Bad (S99) Antibody
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
IF, DB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)

Alpha Synuclein, Monoclonal Antibody (Cat# AAA808920)

Full Name
Alpha Synuclein Antibody, Clone 3C11: ATTO 594
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human; Mouse; Rat
Applications
WB, DB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunohistochemistry (IHC) (Sample Type: Mouse GutApplication: ImmunohistochemistrySpecies+tissue/cell type: Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStopHow many ug'sof tissue/cell lysate run on the gel: 11 m Mouse Gut TissuePrimary antibody dilution: 1:50Secondary antibody: Goat anti-rabbit-cy3Secondary antibody dilution: 1:1500)

GRIA2, Polyclonal Antibody (Cat# AAA3224492)

Full Name
GRIA2 antibody - N-terminal region
Gene Names
GRIA2; GLUR2; GLURB; GluA2; HBGR2; GluR-K2
Reactivity
Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Protein A purified
Pricing
$390/0.1 mL | $1,750/5x0.1 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)

Rubicon, Polyclonal Antibody (Cat# AAA809917)

Full Name
Rubicon Antibody: HRP
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB) (All lanes : Anti-MAPK12 Antibody (P354) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

MAPK12, Polyclonal Antibody (Cat# AAA9214530)

Full Name
MAPK12 Antibody (Center)
Gene Names
MAPK12; ERK3; ERK6; ERK-6; SAPK3; PRKM12; SAPK-3; MAPK 12; P38GAMMA
Reactivity
Human, mouse
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Acrolein, Monoclonal Antibody (Cat# AAA808585)

Full Name
Acrolein Antibody, Clone 2H2: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Western Blot (WB) (NME2 Antibody (F40) western blot analysis in HL-60,Jurkat,MCF-7 cell line,mouse kidney and rat stomach tissue lysates (35ug/lane).This demonstrates the NME2 antibody detected the NME2 protein (arrow).)

NME2, Polyclonal Antibody (Cat# AAA9204159)

Full Name
NME2 Antibody (N-term)
Gene Names
NME1-NME2; NMELV; NM23-LV
Reactivity
Human, mouse, rat
Applications
EIA, IHC, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)

Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000280)

Full Name
Cyclooxygenase 1 Antibody: PerCP
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB) (Anti-SPDYA Antibody (Center) at 1:1000 dilution + PC-3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

SPDYA, Polyclonal Antibody (Cat# AAA9217174)

Full Name
SPDYA Antibody (Center)
Gene Names
SPDYA; SPY1; SPDY1; RINGO3; RINGOA
Reactivity
Human; Predicted Reactivity: Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

OLIG3, Polyclonal Antibody (Cat# AAA249939)

Full Name
Anti-OLIG3 Antibody (aa80-109) IHC-plus
Gene Names
OLIG3; Bhlhb7; bHLHe20
Reactivity
Human, Mouse
Applications
IHC, WB, FC/FACS
Purity
Protein A purified
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Western Blot (WB) (All lanes : Anti-PLIN3 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: Daudi whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

PLIN3, Monoclonal Antibody (Cat# AAA9217030)

Full Name
PLIN3 Antibody
Gene Names
PLIN3; PP17; TIP47; M6PRBP1
Reactivity
Human
Applications
WB, EIA
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB) (Phospho-HER4-Y1162 Antibody was used to detect Phospho-HER4-Y1162 in FG pancreatic carcinoma cell line lysate.FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 minPhospho-HER4-Y1162 Antibody was used at 1:750 in 3% BSA.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)

Phospho-HER4 (Y1162), Polyclonal Antibody (Cat# AAA9203212)

Full Name
Phospho-HER4 (Y1162) Antibody
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
WB, EIA, IHC, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysates from mouse thymus and rat thymus tissue lysate(from left to right), using LSK Antibody (I37). MBS9204040 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

LCK, Polyclonal Antibody (Cat# AAA9204040)

Full Name
LCK Antibody (N-term)
Gene Names
LCK; LSK; YT16; IMD22; p56lck; pp58lck
Reactivity
Human, mouse
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808666)

Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5E8: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Malondialdehyde, Monoclonal Antibody (Cat# AAA808781)

Full Name
Malondialdehyde Antibody, Clone 11E3: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808646)

Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5D9
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

4-Hydroxy-2-hexenal, Monoclonal Antibody (Cat# AAA808691)

Full Name
4-Hydroxy-2-hexenal Antibody, Clone 6F10: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Western Blot (WB) (Western blot analysis of anti-hFGFR2-C808 Pab in 293 cell line lysates transiently transfected with the FGFR2 gene (2ug/lane). FGFR2 (arrow) was detected using the purified Pab.)

FGFR2, Polyclonal Antibody (Cat# AAA9212732)

Full Name
FGFR2 Antibody (C-term)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with AIFM1 (N-term) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9201935 AIFM1 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). AIFM1 (N-term) immunoreactivity is localized to the cytoplasm of U251 cells.)

AIFM1, Polyclonal Antibody (Cat# AAA9201935)

Full Name
AIFM1 Antibody (N-term)
Gene Names
AIFM1; AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6
Reactivity
Human
Applications
FC/FACS, IF, WB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry - Paraffin (IHC) (Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

CDH1 / E Cadherin, Polyclonal Antibody (Cat# AAA249742)

Full Name
Anti-CDH1 / E Cadherin Antibody IHC-plus
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Reactivity
Mouse, Dog, Rat, Human, Monkey
Applications
IHC, IHC, WB
Purity
Immunoaffinity purified
Pricing
$585/0.3 mg | $2,585/5x0.3 mg
Western Blot (WB)

Aquaporin 10, Polyclonal Antibody (Cat# AAA540570)

Full Name
Aquaporin 10 Antibody
Gene Names
AQP10; AQPA_HUMAN
Reactivity
Human, Mouse, Rat
Applications
EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB) (Western blot analysis of hEPHA2-T45 in MCF-7 cell line lysates (35ug/lane). EPHA2 (arrow) was detected using the purified Pab)

EphA2, Polyclonal Antibody (Cat# AAA9209771)

Full Name
EphA2 Antibody (N-term)
Gene Names
EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
WB, EIA, FC/FACS, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (UCHL1 Antibody (C-term) western blot analysis in U266,SH-SY5Y,NCI-H1299,mouse Neuro-2a,rat C6,PC-12 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).)

UCHL1, Polyclonal Antibody (Cat# AAA9214247)

Full Name
UCHL1 Antibody (C-term)
Gene Names
UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Monkey, Pig, Rat)
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Acrolein, Monoclonal Antibody (Cat# AAA808592)

Full Name
Acrolein Antibody, Clone 10A10
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Malondialdehyde, Monoclonal Antibody (Cat# AAA808769)

Full Name
Malondialdehyde Antibody, Clone 6H6: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Immunofluorescence (IF) (MBS9605806 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

RED, Polyclonal Antibody (Cat# AAA9605806)

Full Name
RED Antibody
Gene Names
IK; RED; RER; CSA2
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Anti-TERT / Telomerase antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

TERT / Telomerase, Polyclonal Antibody (Cat# AAA2400244)

Full Name
Anti-TERT / Telomerase Antibody (aa627-656) IHC-plus
Gene Names
TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
Reactivity
Human
Applications
IHC, IF, WB, FC/FACS
Purity
Ammonium sulfate precipitation
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.)

Cyclooxygenase 1, Polyclonal Antibody (Cat# AAA8000265)

Full Name
Cyclooxygenase 1 Antibody
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Western Blot (WB) (Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121). MBS9207828 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Connexin 43, Polyclonal Antibody (Cat# AAA9207828)

Full Name
Connexin 43 Antibody (N-term)
Gene Names
GJA1; HSS; CMDR; CX43; GJAL; ODDD; AVSD3; HLHS1
Reactivity
Human, mouse, rat (Predicted Reactivity: Bovine, Xenopus)
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC) (Anti-IL-33 antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IL33, Polyclonal Antibody (Cat# AAA242172)

Full Name
Rabbit Polyclonal (IgG) to Human IL33
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Reactivity
Human
Applications
IHC - Paraffin, WB, EIA
Purity
Immunoaffinity Purified
Pricing
$585/0.05 mg | $2,585/5x0.05 mg
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dibromo-tyrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Dibromo-tyrosine, Monoclonal Antibody (Cat# AAA808640)

Full Name
Dibromo-tyrosine Antibody, Clone 9F12: FITC
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,235/5x0.1 mg
Western Blot (WB) (Western blot analysis of lysate from HepG2 cell line, using ACVR1 Antibody (R147). MBS9208699 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Activin Receptor Type IA (ACVR1), Polyclonal Antibody (Cat# AAA9208699)

Full Name
Activin Receptor Type IA (ACVR1) Antibody (Center R147)
Gene Names
ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (All lanes : Anti-Phospho-GIT1-pY554. ctrl at 1:2000 dilutionLane 1: U-2OS whole cell lysateLane 2: HUVEC whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

GIT1, Polyclonal Antibody (Cat# AAA9217143)

Full Name
GIT1 (Y554) Antibody (C-term)
Reactivity
Human, Mouse; Predicted Reactivity: Rat
Applications
WB, FC/FACS, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)

VPS35, Monoclonal Antibody (Cat# AAA8005649)

Full Name
VPS35 Antibody, Clone 8A3: Biotin
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$535/0.1 mg | $2,170/5x0.1 mg
Western Blot (WB) (All lanes : Anti-MAP3K5 Antibody (T842) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Raji whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 155 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

MAP3K5, Polyclonal Antibody (Cat# AAA9217159)

Full Name
MAP3K5 Antibody(T842)
Gene Names
MAP3K5; ASK1; MEKK5; MAPKKK5
Reactivity
Human; Predicted Reactivity: Mouse
Applications
WB, IHC, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)

Alpha Synuclein, Monoclonal Antibody (Cat# AAA808917)

Full Name
Alpha Synuclein Antibody, Clone 3C11: ATTO 390
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human; Mouse; Rat
Applications
WB, DB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)

Alpha Synuclein, Monoclonal Antibody (Cat# AAA808956)

Full Name
Alpha Synuclein Antibody, Clone 3F8: ATTO 594
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB) (ENASE Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ENASE antibody detected the ENASE protein (arrow).)

ENASE, Polyclonal Antibody (Cat# AAA9214338)

Full Name
ENASE Antibody (Center)
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (HNRPAB Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HNRPAB antibody detected the HNRPAB protein (arrow).)

HNRPAB, Polyclonal Antibody (Cat# AAA9205445)

Full Name
HNRPAB Antibody (N-term)
Gene Names
HNRNPAB; ABBP1; HNRPAB
Reactivity
Human
Applications
EIA, IHC, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) ((0.5ug/ml) staining of MEF lysates (35ug protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.)

MK2/MAPKAPK2, Polyclonal Antibody (Cat# AAA422978)

Full Name
Goat anti-MK2/MAPKAPK2 Antibody
Gene Names
MAPKAPK2; MK2; MK-2; MAPKAP-K2
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
EIA, WB, IP
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB) (TSN Antibody (Center) western blot analysis in HL-60,Jurkat,K562 cell line mouse spleen tissue lysates (35ug/lane).This demonstrates the TSN antibody detected the TSN protein (arrow).)

TSN, Polyclonal Antibody (Cat# AAA9214736)

Full Name
TSN Antibody (Center)
Gene Names
TSN; C3PO; RCHF1; TBRBP; TRSLN; BCLF-1; REHF-1
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Hamster)
Applications
EIA, IHC, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

4-Hydroxy-2-hexenal, Monoclonal Antibody (Cat# AAA808694)

Full Name
4-Hydroxy-2-hexenal Antibody, Clone 6F10: FITC
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,235/5x0.1 mg
Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647 (MBS215657A647))

CD206, Monoclonal Antibody (Cat# AAA215668)

Full Name
RAT ANTI MOUSE CD206:RPE
Gene Names
Mrc1; MR; CD206; AW259686
Applications
FC/FACS
Pricing
$255/25 Tests | $995/5x25 Tests
Western Blot (WB) (All lanes : Anti-SLC16A10 Antibody (C-term) at 1:2000 dilutionLane 1: human skeletal muscle lysatesLane 2: CCRF-CEM whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

SLC16A10, Polyclonal Antibody (Cat# AAA9205038)

Full Name
SLC16A10 Antibody (C-term)
Gene Names
SLC16A10; TAT1; MCT10; PRO0813
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
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