Western Blot (WB)
(All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilutionLane 1: human brain lysateLane 2: mouse liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse anti-Human, Mouse GLS2 Monoclonal Antibody | anti-GLS2 antibody
GLS2 Antibody
IHC-P: ~~1:25
Western Blot (WB)
(All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilutionLane 1: human brain lysateLane 2: mouse liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilutionLane 1: human brain lysateLane 2: mouse liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry-Paraffin (IHC-P)
(MBS9230948 staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry-Paraffin (IHC-P)
(MBS9230948 staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
Uniprot Description
GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; EC 3.5.1.2; Energy Metabolism - nitrogen; Hydrolase; Mitochondrial; Other Amino Acids Metabolism - D-Glutamine and D-glutamate
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: mitochondrial matrix; mitochondrion
Molecular Function: glutaminase activity; protein binding
Biological Process: amino acid biosynthetic process; amino acid metabolic process; glutamate biosynthetic process; glutamate secretion; glutamine catabolic process; regulation of apoptosis
Research Articles on GLS2
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Product Notes
The GLS2 gls2 (Catalog #AAA9230948) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GLS2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Imunnohistochemistry-Paraffin (IHC-P). WB: 1:1000-1:2000 IHC-P: ~~1:25. Researchers should empirically determine the suitability of the GLS2 gls2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLS2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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