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Western Blot (WB) (Anti-FAT4 Antibody at 1:2000 dilution + Recombinant proteinLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 543 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Mouse anti-Human FAT4 Monoclonal Antibody | anti-FAT4 antibody

FAT4 Antibody

Gene Names
FAT4; FATJ; FAT-J; CDHF14; CDHR11; HKLLS2; VMLDS2; NBLA00548
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
FAT4; Monoclonal Antibody; FAT4 Antibody; Protocadherin Fat 4; hFat4; Cadherin family member 14; FAT tumor suppressor homolog 4; Fat-like cadherin protein FAT-J; CDHF14; FATJ; anti-FAT4 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Specificity
This FAT4 antibody is generated from a mouse immunized with a recombinant protein between 4500-4786 amino acids from human FAT4.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
4982
Applicable Applications for anti-FAT4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:2000
Subtitle
Purified Mouse Monoclonal Antibody (Mab)
Cellular Location
Membrane; Single-pass type I membrane protein. Note=In the kidney, localizes to primary cilia.
Clone Names
1654CT645.1.70
Tissue Location
Widely expressed. Expressed in fetal brain, infant brain, brain tumor and colorectal cancer.
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-FAT4 Antibody at 1:2000 dilution + Recombinant proteinLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 543 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-FAT4 Antibody at 1:2000 dilution + Recombinant proteinLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 543 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-FAT4 Antibody at 1:2000 dilution + Recombinant proteinLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 543 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-FAT4 Antibody at 1:2000 dilution + Recombinant proteinLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 543 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-FAT4 Antibody at 1:2000 dilution + Recombinant proteinLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 543 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-FAT4 Antibody at 1:2000 dilution + Recombinant proteinLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 543 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-FAT4 antibody
Cadherins are calcium-dependent cell adhesion proteins. FAT4 plays a role in the maintenance of planar cell polarity as well as in inhibition of YAP1-mediated neuroprogenitor cell proliferation and differentiation (By similarity).
Product Categories/Family for anti-FAT4 antibody
References
Hoeng J.C.,et al.J. Mol. Biol. 337:307-317 (2004). Hillier L.W.,et al.Nature 434:724-731 (2005). Ohira M.,et al.Cancer Lett. 197:63-68 (2003). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Bechtel S.,et al.BMC Genomics 8:399-399 (2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
542687 Da
NCBI Official Full Name
protocadherin Fat 4 isoform 2
NCBI Official Synonym Full Names
FAT atypical cadherin 4
NCBI Official Symbol
FAT4
NCBI Official Synonym Symbols
FATJ; FAT-J; CDHF14; CDHR11; HKLLS2; VMLDS2; NBLA00548
NCBI Protein Information
protocadherin Fat 4
UniProt Protein Name
Protocadherin Fat 4
Protein Family
UniProt Gene Name
FAT4
UniProt Synonym Gene Names
CDHF14; FATJ; hFat4
UniProt Entry Name
FAT4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protocadherin family. This gene may play a role in regulating planar cell polarity (PCP). Studies in mice suggest that loss of PCP signaling may cause cystic kidney disease, and mutations in this gene have been associated with Van Maldergem Syndrome 2. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Mar 2014]

Uniprot Description

FAT4: May function in the regulation of planar cell polarity. Cadherins are cell-cell interaction molecules. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 4q28.1

Cellular Component: apical part of cell; integral to membrane; intracellular; plasma membrane

Molecular Function: calcium ion binding; protein binding

Biological Process: cerebral cortex development; gut development; heart morphogenesis; heterophilic cell adhesion; homophilic cell adhesion; inner ear receptor stereocilium organization and biogenesis; neurogenesis; plasma membrane organization and biogenesis; ureteric bud branching

Disease: Hennekam Lymphangiectasia-lymphedema Syndrome 2; Van Maldergem Syndrome 2

Research Articles on FAT4

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Product Notes

The FAT4 fat4 (Catalog #AAA9217029) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FAT4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAT4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the FAT4 fat4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAT4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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