Human Spondin 1 (SPON1) ELISA Kit | SPON1 elisa kit

Human Spondin 1 (SPON1) ELISA Kit

Cat. Num. AAA2019780

• Gene Names: SPON1; f-spondin; VSGP/F-spondin
• Reactivity: Human
• Synonyms: Spondin 1 (SPON1); Human Spondin 1 (SPON1) ELISA Kit; VSGP; F-spondin; Extracellular Matrix Protein; Vascular smooth muscle cell growth-promoting factor; SPON1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Spondin 1 (SPON1).; No significant cross-reactivity or interference between Spondin 1 (SPON1) and analogues was observed.
• Sequence Length: 807

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.235ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spondin 1 (SPON1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spondin 1 (SPON1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPON1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spondin 1 (SPON1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spondin 1 (SPON1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spondin 1 (SPON1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spondin 1 (SPON1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SPON1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 110347423
• NCBI GeneID: 10418
• NCBI Accession #: NP_006099.2
• NCBI GenBank Nucleotide #: NM_006108.3
• Molecular Weight: 90,973 Da
• NCBI Official Full Name: spondin-1
• NCBI Official Synonym Full Names: spondin 1, extracellular matrix protein
• NCBI Official Symbol: SPON1
• NCBI Official Synonym Symbols: f-spondin; VSGP/F-spondin
• NCBI Protein Information: spondin-1
• UniProt Protein Name: Spondin-1
• Protein Family: Spondin
• UniProt Gene Name: SPON1
• UniProt Synonym Gene Names: KIAA0762; VSGP
• UniProt Entry Name: SPON1_HUMAN

Uniprot Description

SPON1: Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum lumen

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; cell adhesion; post-translational protein modification