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Application
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6044 results for " Fluorescent" - showing 350-400
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using HSP70 antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
HSP70, Monoclonal Antibody (Cat# AAA5310895)
Full Name
HSP70 antibody
Applications
EIA, IF, IHC, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis using ISL1 mouse mAb against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).)
ISL1, Antibody (Cat# AAA850661)
Full Name
ISL1 Antibody
Gene Names
ISL1; Isl-1; ISLET1
Reactivity
Human
Applications
WB, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of lysates from U266,SH-SY5Y,mouse Neuro-2a cell line,mouse brain,rat brain tissue lysate,NCI-H460 cell line (from left to right), using UCHL1 Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
UCHL1, Monoclonal Antibody (Cat# AAA9231780)
Full Name
UCHL1 Antibody
Gene Names
UCHL1; NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from Hela, K562, MCF-7 cell line (from left to right), using EEF1B2 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
EEF1B2, Polyclonal Antibody (Cat# AAA9231626)
Full Name
EEF1B2 Antibody (Center)
Gene Names
EEF1B2; EF1B; EEF1B; EEF1B1
Reactivity
Human
Predicted: Chicken
Predicted: Chicken
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of HepG2 cells stained with CTSD. MBS9215107 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
CTSD, Polyclonal Antibody (Cat# AAA9215107)
Full Name
CTSD Antibody
Gene Names
CTSD; CPSD; CLN10; HEL-S-130P
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western Blot showing CDH2 antibody used against A431 (1), NIH/3T3 (2), Hela (3), C6 (4) and LNCap (5) cell lysate.)
CDH2, Monoclonal Antibody (Cat# AAA831446)
Full Name
CDH2 antibody
Gene Names
CDH2; CDHN; NCAD; CD325; CDw325
Applications
EIA, IF, IHC, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of K562 cells using CDK5 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9208751 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
CDK5, Polyclonal Antibody (Cat# AAA9208751)
Full Name
CDK5 Antibody (C-term)
Gene Names
CDK5; LIS7; PSSALRE
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat, Xenopus)
Applications
FC/FACS, IF, WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with POLDIP3 Antibody (N-term). MBS9213858 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
POLDIP3, Polyclonal Antibody (Cat# AAA9213858)
Full Name
POLDIP3 Antibody (N-term)
Gene Names
POLDIP3; SKAR; PDIP46
Reactivity
Human, mouse
Applications
IF, EIA, IHC, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(All lanes : Anti-Smarcc1 Antibody (C-term) at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: C2C12 whole cell lysatesLane 3: C6 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 123 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Smarcc1, Polyclonal Antibody (Cat# AAA9231979)
Full Name
(Mouse) Smarcc1 Antibody (C-term)
Gene Names
Smarcc1; Rsc8; SRG3; msp3; BAF155; AI115498
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with SREBF2 Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SREBF2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).SREBF2 immunoreactivity is localized to Nucleus and Nucleolus significantly.)
SREBF2, Polyclonal Antibody (Cat# AAA9206806)
Full Name
SREBF2 Antibody (Center)
Gene Names
SREBF2; SREBP2; bHLHd2; SREBP-2
Reactivity
Human
Applications
EIA, IHC, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells stained with ERAS (N-term) (F66) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9207630 ERAS (N-term) (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.)
ERAS, Polyclonal Antibody (Cat# AAA9207630)
Full Name
ERAS Antibody (N-term) (F66)
Gene Names
ERAS; HRAS2; HRASP
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing SH-SY5Y cells stained with (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Alexa Fluor 488 goat anti-mouse lgG (166821) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was mouse IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
CHRM2, Monoclonal Antibody (Cat# AAA9232024)
Full Name
CHRM2 Antibody
Gene Names
CHRM2; HM2
Reactivity
Human, Mouse
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215793)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA211045)
Full Name
RAT ANTI HUMAN CD18:FITC
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS
Pricing
$415/100 Tests | $1,710/5x100 Tests
Western Blot (WB)
(Figure 1: Western blot analysis using TNFRSF11B mAb against HEK293 (1) and TNFRSF11B-hIgGFc transfected HEK293 (2) cell lysate.)
TNFRSF11B, Antibody (Cat# AAA850438)
Full Name
TNFRSF11B Antibody
Gene Names
TNFRSF11B; OPG; TR1; OCIF; PDB5
Reactivity
Human
Applications
WB, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Figure 1: Flow cytometric analysis of 3T3L1 cells using BCL-2 mouse mAb (green) and negative control (purple).)
BCL-2, Antibody (Cat# AAA850559)
Full Name
BCL-2 Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse
Applications
WB, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Figure 1. Western blot analysis using KDR mouse mAb against extracellular domain of human KDR (aa20-764).)
WNT10B, Antibody (Cat# AAA850938)
Full Name
WNT10B Antibody
Gene Names
WNT10B; SHFM6; STHAG8; WNT-12
Reactivity
Human
Applications
WB, IHC, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of lysates from MCF-7, PC-3, K562 cell line (from left to right), using PSMD10 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. )
PSMD10, Polyclonal Antibody (Cat# AAA9231701)
Full Name
PSMD10 Antibody (Center)
Gene Names
PSMD10; p28; p28(GANK); dJ889N15.2
Reactivity
Human
Predicted: Mouse
Predicted: Mouse
Applications
WB, IHC, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MCF-7 cells using AGAP8 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9206649 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
AGAP8, Polyclonal Antibody (Cat# AAA9206649)
Full Name
AGAP8 Antibody (C-term)
Reactivity
Human
Applications
WB, EIA, FC/FACS, IF, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded M. brain section using Mouse Actl6a Antibody (C-term). MBS9206805 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Actl6a, Polyclonal Antibody (Cat# AAA9206805)
Full Name
Mouse Actl6a Antibody (C-term)
Gene Names
Actl6a; ARP4; Actl6; Baf53a; C79802; AI851094; 2810432C06Rik
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
EIA, IHC, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from K562 cell line,zebra fish brain,mouse brain,mouse testis tissue lysate,PC-3 cell line (from left to right), using DMRTA2 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
DMRTA2, Polyclonal Antibody (Cat# AAA9231680)
Full Name
DMRTA2 Antibody (C-term)
Reactivity
Human, Mouse, Zebrafish
Predicted: Bovine
Predicted: Bovine
Applications
WB, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of BARON antibody (N-term) in MCF-7 cell line lysates (35ug/lane). BARON (arrow) was detected using the purified Pab.)
BARON, Polyclonal Antibody (Cat# AAA9203134)
Full Name
BARON Antibody (N-term)
Gene Names
KIAA0226; SCAR15; RUBICON
Reactivity
Human
Applications
WB, EIA, IF, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF)
(Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
ISL1, Monoclonal Antibody (Cat# AAA5306525)
Full Name
Mouse monoclonal ISL1 antibody
Gene Names
ISL1; Isl-1; ISLET1
Applications
EIA, IF, IHC, WB
Pricing
$645/0.1 mL | $2,885/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis using EphB4 mouse mAb against extracellular domain of human EphB4 (aa16-539).)
EPHB4, Antibody (Cat# AAA850950)
Full Name
EPHB4 Antibody
Gene Names
EPHB4; HTK; MYK1; HFASD; TYRO11
Reactivity
Human
Applications
WB, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(All lanes : Anti-Pink1 Antibody at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 63 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
PINK1, Monoclonal Antibody (Cat# AAA9232023)
Full Name
PINK1 Antibody
Gene Names
PINK1; BRPK; PARK6
Reactivity
Human, Mouse
Applications
WB, IHC, IF, EIA
Pricing
$445/0.1 mL | $1,695/5x0.1 mL
Western Blot (WB)
(TBPL2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TBPL2 antibody detected the TBPL2 protein (arrow).)
TBPL2, Polyclonal Antibody (Cat# AAA9208570)
Full Name
TBPL2 Antibody (N-term)
Gene Names
TBPL2; TBP2; TRF3
Reactivity
Human
Applications
WB, EIA, IHC, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(NCL Antibody (Center E443) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow).)
NCL, Polyclonal Antibody (Cat# AAA9201499)
Full Name
NCL Antibody (Center E443)
Gene Names
NCL; C23
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
WB, EIA, IHC, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(NR3C1 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NR3C1 antibody detected NR3C1 protein (arrow).)
NR3C1, Polyclonal Antibody (Cat# AAA9207237)
Full Name
NR3C1 Antibody (Center)
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human, mouse (Predicted Reactivity: Rabbit)
Applications
WB, EIA, IHC, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysate from U-251 MG cell line, using YBX1 Antibody (C-term). MBS9202479 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
YBX1, Polyclonal Antibody (Cat# AAA9202479)
Full Name
YBX1 Antibody (C-term)
Gene Names
YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1
Reactivity
Human (Predicted Reactivity: Xenopus, Bovine, Mouse, Rat)
Applications
WB, EIA, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(All lanes : Anti-GNL3 Antibody (N-term) at 1:1000 dilutionLane 1: F9 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: K562 whole cell lysatesLane 4: Raji whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
GNL3, Polyclonal Antibody (Cat# AAA9231984)
Full Name
GNL3 Antibody (N-term)
Gene Names
GNL3; NS; E2IG3; NNP47; C77032
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
WB, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from Hela,K562,MCF-7,mouse NIH/3T3 cell line (from left to right), using NEK2 Antibody (C410). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
NEK2, Polyclonal Antibody (Cat# AAA9231753)
Full Name
NEK2 Antibody (Center)
Gene Names
NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
Reactivity
Human, Mouse
Applications
WB, IHC, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent confocal image of HeLa cells stained with PDK4 (C-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9214973 PDK4 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.)
PDK4, Polyclonal Antibody (Cat# AAA9214973)
Full Name
PDK4 Antibody (C-term)
Reactivity
Human, mouse
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(All lanes : Anti-HDAC1 Antibody (N-term) at 1:1000 dilutionLane 1: mouse uterus lysatesLane 2: MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 60 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
HDAC1, Polyclonal Antibody (Cat# AAA9231665)
Full Name
HDAC1 Antibody (N-term)
Gene Names
HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1
Reactivity
Human, Mouse, Rat
Predicted: Xenopus, Bovine, Chicken
Predicted: Xenopus, Bovine, Chicken
Applications
WB, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with NR1H3 Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR1H3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).NR1H3 immunoreactivity is localized to Nucleus significantly.)
NR1H3, Polyclonal Antibody (Cat# AAA9212029)
Full Name
NR1H3 Antibody (Center)
Gene Names
NR1H3; LXRA; LXR-a; RLD-1
Reactivity
Human
Applications
FC/FACS, IF, WB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(HDAC4 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow).)
HDAC4, Polyclonal Antibody (Cat# AAA9201247)
Full Name
HDAC4 Antibody (C-term)
Gene Names
HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of TSH2 Antibody (N-term) in CEM cell line lysates (35ug/lane).TSH2 (arrow) was detected using the purified Pab.)
TSH2, Polyclonal Antibody (Cat# AAA9202384)
Full Name
TSH2 Antibody (N-term)
Gene Names
TSHZ2; TSH2; ZABC2; ZNF218; OVC10-2; C20orf17
Reactivity
Human (Predicted Reactivity: Mouse, Pig)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from SH-SY5Y,Hela cell line (from left to right), using HDAC2 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
HDAC2, Polyclonal Antibody (Cat# AAA9231833)
Full Name
HDAC2 Antibody (Center)
Gene Names
HDAC2; HD2; RPD3; YAF1; KDAC2
Reactivity
Human
Predicted: Chicken, Mouse
Predicted: Chicken, Mouse
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS)
(FC analysis of K562 cells using CHD3 antibody (green) and negative control (purple).)
CHD3, Monoclonal Antibody (Cat# AAA5310886)
Full Name
CHD3 antibody
Gene Names
Chd3; CG9594; CHD-3; chd3; CHD3; dCHD3; DmCHD3; DmelCG9594
Applications
EIA, IF, IHC, WB
Pricing
$605/0.1 mg | $2,715/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis using NGFR mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.)
NGFR, Antibody (Cat# AAA850009)
Full Name
NGFR Antibody
Gene Names
NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
Reactivity
Human
Applications
WB, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of anti-Phospho-STAT5 Pab in HepG2 cell line lysate (35ug/lane). Phospho-STAT5(arrow) was detected using the purified Pab.)
Phospho-STAT5a (Y694), Polyclonal Antibody (Cat# AAA9211000)
Full Name
Phospho-STAT5a (Y694) Antibody
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human
Applications
WB, EIA, IF, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9213526 ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG12, Polyclonal Antibody (Cat# AAA9213526)
Full Name
ATG12 Antibody (N-term)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of SH-SY5Y cells stained with SUMO2/3 Antibody (C-term). MBS9202693 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
SUMO2/3, Polyclonal Antibody (Cat# AAA9202693)
Full Name
SUMO2/3 Antibody (C-term)
Gene Names
SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
Reactivity
Human, mouse (Predicted Reactivity: Xenopus, Zebrafish, Bovine, Chicken, Hamster, Monkey, Pig, Rat)
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts from A431 cells,untreated or treated with EGF,100ng/ml?using phospho ERBB2 Y1196 (left) or ERBB2 antibody(right))
Phospho-ERBB2 (Y1196), Polyclonal Antibody (Cat# AAA9211709)
Full Name
Phospho-ERBB2 (Y1196) Antibody
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human
Applications
IF, DB, EIA, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(USF1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow).)
USF1, Polyclonal Antibody (Cat# AAA9212224)
Full Name
USF1 Antibody (Center)
Gene Names
USF1; UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
WB, EIA, FC/FACS, IF, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Texas red conjugated Goat anti Mouse IgG antibody used for the detection of bound anti CD80 antibody by immunofluorescenceImage caption:Immunolabelling of Mphi by the monocyte/Mphi marker CD14 and the M1 Mphi marker CD80. Mphi were seeded at a density of 5.7×104 cells/cm2, with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 mug/ml LPS and 5 ng/ml IFNgamma for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mphi cultured alone and was not detectable on Mphi in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C.Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.BMC Cancer. 2010 Jul 17;10:375)
IgG, Polyclonal Antibody (Cat# AAA225563)
Full Name
Goat Anti Mouse IgG: FITC
Reactivity
Mouse
Applications
FC/FACS, IF
Purity
Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
Pricing
$250/1 mg | $975/5x1 mg
GFP-Tag, Monoclonal Antibody (Cat# AAA126137)
Full Name
GFP-Tag Monoclonal Antibody
Applications
WB, IF
Purity
Affinity Purification
Pricing
$175/0.05 mL | $215/0.1 mL | $285/0.2 mL | $1,130/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis using human IgG (Fc specific) mouse mAb against different fusion proteins with human IgG(Fc specific) tag. Figure 1.)
IgGFc, Antibody (Cat# AAA850183)
Full Name
human IgGFc Antibody
Reactivity
Human
Applications
WB, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis using GUCY1A3 mouse mAb against HEK293 (1) and Raji (2) cell lysate.)
GUCY1A3, Monoclonal Antibody (Cat# AAA476665)
Full Name
Anti-GUCY1A3 Mouse mAb
Gene Names
GUCY1A3; GUCA3; MYMY6; GC-SA3; GUC1A3; GUCSA3; GUCY1A1
Reactivity
Human
Applications
WB, ICC, IHC, FC/FACS
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Figure 1: Western blot analysis using MAP2K6 mouse mAb against HepG2 (1), MCF-7 (2) and NIH/3T3 (3) cell lysate.)
MAP2K6, Antibody (Cat# AAA850505)
Full Name
MAP2K6 Antibody
Gene Names
MAP2K6; MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
Reactivity
Human, Mouse
Applications
WB, IHC, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Figure 1: Western blot analysis using GABPA mouse mAb against Hela (1), A549 (2), MCF-7 (3), NIH/3T3 (4) and SMMC-7721 (5) cell lysate.)
GABPA, Antibody (Cat# AAA850800)
Full Name
GABPA Antibody
Gene Names
GABPA; NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
Reactivity
Human, Mouse
Applications
WB, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
