Rabbit Actl6a Polyclonal Antibody | anti-Actl6a antibody

Mouse Actl6a Antibody (C-term)

Cat. Num. AAA9206805

• Gene Names: Actl6a; ARP4; Actl6; Baf53a; C79802; AI851094; 2810432C06Rik
• Reactivity: Human (Predicted Reactivity: Monkey)
• Applications: ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Actl6a; Polyclonal Antibody; Mouse Actl6a Antibody (C-term); Actin-like protein 6A; 53 kDa BRG1-associated factor A; Actin-related protein Baf53a; BRG1-associated factor 53A; BAF53A; Actl6; Baf53a; anti-Actl6a antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Monkey)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Actl6a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 295-320 amino acids from the C-terminal region of Mouse Actl6a.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 429

• Applicable Applications for anti-Actl6a antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IHC~~1:25; IF~~1:25; WB~~1:1000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded M. brain section using Mouse Actl6a Antibody (C-term). MBS9206805 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Immunofluorescence (IF)

(Fluorescent image of Hela cells stained with Mouse Actl6a Antibody (C-term). MBS9206805 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

Western Blot (WB)

(Western blot analysis of lysates from K562, Hela cell line (from left to right), using Actl6a Antibody (C-term). MBS9206805 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Related Product Information for anti-Actl6a antibody

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair (By similarity).

Product Categories/Family for anti-Actl6a antibody

Crown Antibodies; Signal Transduction; Stem Cells

References

Zhao K.,et al.Cell 95:625-636(1998).
Carninci P.,et al.Science 309:1559-1563(2005).
Lessard J.,et al.Neuron 55:201-215(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 189181668
• NCBI GeneID: 56456
• NCBI Accession #: NP_062647.2
• NCBI GenBank Nucleotide #: NM_019673.2
• UniProt Accession #: Q9Z2N8; Q8C1W3; Q99M56
• Molecular Weight: 47448
• NCBI Official Full Name: actin-like protein 6A
• NCBI Official Synonym Full Names: actin-like 6A
• NCBI Official Symbol: Actl6a
• NCBI Official Synonym Symbols: ARP4; Actl6; Baf53a; C79802; AI851094; 2810432C06Rik
• NCBI Protein Information: actin-like protein 6A
• UniProt Protein Name: Actin-like protein 6A
• Protein Family: Actin-like protein
• UniProt Gene Name: Actl6a
• UniProt Synonym Gene Names: Actl6; Baf53a; BAF53A
• UniProt Entry Name: ACL6A_MOUSE

Uniprot Description

BAF53A: an actin-related protein involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of the helicase SMARCA4 and for association of the SMARCA4 containing remodelling complex BAF with chromatin/nuclear matrix. A component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of specific genes. Two alternatively spliced human isoforms have been described.

Cellular Component: SWI/SNF complex; NuA4 histone acetyltransferase complex; protein complex; nuclear chromatin; plasma membrane; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; chromatin binding

Biological Process: chromatin remodeling; nervous system development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of growth; ATP-dependent chromatin remodeling; chromatin modification; DNA repair; response to DNA damage stimulus; DNA recombination