Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
6044 results for " Fluorescent" - showing 200-250
Immunofluorescence (IF)
(Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9200115 MET/HGFR primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976).)
MET/HGFR, Monoclonal Antibody (Cat# AAA9200115)
Full Name
MET/HGFR Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human, mouse
Applications
IF, EIA, IHC, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Figure 1: Western blot analysis using FGFR4 mouse mAb against extracellular domain of human FGFR4 (aa22-369). Figure 1: West Figure 1:)
FGFR4, Antibody (Cat# AAA850611)
Full Name
FGFR4 Antibody
Gene Names
FGFR4; TKF; JTK2; CD334
Reactivity
Human
Applications
WB, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Anti-WNT5A antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
WNT5A, Monoclonal Antibody (Cat# AAA246476)
Full Name
Mouse Monoclonal [clone 6F2] (IgG1) to Human WNT5A
Gene Names
WNT5A; hWNT5A
Reactivity
Human
Applications
IHC - Paraffin, IF, WB, EIA
Purity
Ascites
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Immunofluorescence (IF)
(Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
HSP27, Monoclonal Antibody (Cat# AAA9602776)
Full Name
HSP27 Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human, Rat
Applications
WB, IHC, IF, ICC, EIA, FC/FACS
Purity
Affinity-Chromatography
Pricing
$260/0.05 mL | $325/0.1 mL | $400/0.2 mL | $1,750/5x0.2 mL
Western Blot (WB)
(SUMO2/3 Antibody (C-term E69) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).)
SUMO2/3, Polyclonal Antibody (Cat# AAA9212829)
Full Name
SUMO2/3 Antibody (C-term E69)
Gene Names
SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
Reactivity
Human (Predicted Reactivity: Xenopus, Zebrafish, Bovine, Chicken, Hamster, Monkey, Mouse, Pig, Rat)
Applications
EIA, IHC, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of MCF-7 cell stained with TREF1 Antibody (C-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TREF1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).TREF1 immunoreactivity is localized to Nucleus significantly.)
TREF1, Polyclonal Antibody (Cat# AAA9200686)
Full Name
TREF1 Antibody (C-term)
Gene Names
TRERF1; RAPA; BCAR2; TREP132; TReP-132; HSA277276; dJ139D8.5
Reactivity
Human
Applications
FC/FACS, IF, WB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(OAT Antibody (N-term) western blot analysis in 293,293T cell line,mouse liver and rat liver tissue lysates (35ug/lane).This demonstrates the OAT antibody detected the OAT protein (arrow).)
OAT, Polyclonal Antibody (Cat# AAA9207986)
Full Name
OAT Antibody (N-term)
Gene Names
OAT; OKT; GACR; HOGA; OATASE
Reactivity
Human, mouse, rat
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(NPM1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NPM1 antibody detected the NPM1 protein (arrow).)
NPM1, Polyclonal Antibody (Cat# AAA9202409)
Full Name
NPM1 Antibody (N-term)
Gene Names
NPM1; B23; NPM
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent confocal image of HeLa cells stained with phospho-Bad-S99 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9207687 phospho-Bad-S99 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-Bad-S99 mainly to the cytoplasm.)
Phospho-Bad (S99), Polyclonal Antibody (Cat# AAA9207687)
Full Name
Phospho-Bad (S99) Antibody
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
IF, DB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215795)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$695/0.5 mg | $2,975/5x0.5 mg
Western Blot (WB)
(TRIM28 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow).)
TRIM28, Polyclonal Antibody (Cat# AAA9206535)
Full Name
TRIM28 Antibody (N-term)
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(NME2 Antibody (F40) western blot analysis in HL-60,Jurkat,MCF-7 cell line,mouse kidney and rat stomach tissue lysates (35ug/lane).This demonstrates the NME2 antibody detected the NME2 protein (arrow).)
NME2, Polyclonal Antibody (Cat# AAA9204159)
Full Name
NME2 Antibody (N-term)
Gene Names
NME1-NME2; NMELV; NM23-LV
Reactivity
Human, mouse, rat
Applications
EIA, IHC, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC)
(Anti-BMI-1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
BMI1 / PCGF4, Polyclonal Antibody (Cat# AAA240598)
Full Name
Goat Polyclonal to Human BMI1 / PCGF4
Gene Names
BMI1; PCGF4; RNF51; FLVI2/BMI1
Reactivity
Human
Applications
IHC - Paraffin, ICC, IF, WB, EIA
Purity
Immunoaffinity Purified
Pricing
$640/0.05 mg | $2,815/5x0.05 mg
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using SP1 Antibody(green) compared to an isotype control of mouse IgG1(blue). MBS9200319 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
SP1, Monoclonal Antibody (Cat# AAA9200319)
Full Name
SP1 Antibody
Reactivity
Human
Applications
WB, EIA, FC/FACS, IF
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of U-87 MG cells stained with MORC2 Antibody (C-term). MBS9211160 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
MORC2, Polyclonal Antibody (Cat# AAA9211160)
Full Name
MORC2 Antibody (C-term)
Gene Names
MORC2; ZCW3; ZCWCC1
Reactivity
Human
Applications
WB, EIA, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with PRS4 Antibody (C-term). MBS9211794 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
PRS4, Polyclonal Antibody (Cat# AAA9211794)
Full Name
PRS4 Antibody (C-term)
Gene Names
PSMC1; S4; p56; P26S4
Reactivity
Human, mouse, rat
Applications
IF, EIA, IHC, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(All lanes : Anti-IPF Antibody (C-term) at 1:1000 dilutionLane 1: HepG2 whole cell lysatesLane 2: mouse stomach lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 31 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
IPF, Polyclonal Antibody (Cat# AAA9232017)
Full Name
IPF Antibody (C-term)
Gene Names
PDX1; GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1; PAGEN1
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
WB, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent confocal image of HepG2 cells stained with BNIP3 (BH3 Domain Specific) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9203402 BNIP3 (BH3 Domain Specific) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.)
BNIP3, Polyclonal Antibody (Cat# AAA9203402)
Full Name
BNIP3 Antibody (BH3 Domain Specific)
Gene Names
BNIP3; NIP3
Reactivity
Human, mouse
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with AIFM1 (N-term) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9201935 AIFM1 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). AIFM1 (N-term) immunoreactivity is localized to the cytoplasm of U251 cells.)
AIFM1, Polyclonal Antibody (Cat# AAA9201935)
Full Name
AIFM1 Antibody (N-term)
Gene Names
AIFM1; AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6
Reactivity
Human
Applications
FC/FACS, IF, WB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from A549,Hela,MDA-MB-453 cell line (from left to right), using FAM50AAntibody(C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
FAM50A, Polyclonal Antibody (Cat# AAA9231690)
Full Name
FAM50A Antibody (C-term)
Gene Names
FAM50A; 9F; XAP5; HXC26; HXC-26; DXS9928E
Reactivity
Human
Predicted: Xenopus, Zebrafish, Mouse
Predicted: Xenopus, Zebrafish, Mouse
Applications
WB, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(SIX5 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SIX5 antibody detected the SIX5 protein (arrow).)
SIX5, Polyclonal Antibody (Cat# AAA9213036)
Full Name
SIX5 Antibody (Center)
Gene Names
SIX5; BOR2; DMAHP
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
IF, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(Anti-TERT / Telomerase antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
TERT / Telomerase, Polyclonal Antibody (Cat# AAA2400244)
Full Name
Anti-TERT / Telomerase Antibody (aa627-656) IHC-plus
Gene Names
TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
Reactivity
Human
Applications
IHC, IF, WB, FC/FACS
Purity
Ammonium sulfate precipitation
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216589)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
FC/FACS
Pricing
$210/0.025 mg | $800/5x0.025 mg
Immunohistochemistry (IHC)
(Figure 1. Immunohistochemical analysis of paraffin-embedded human lung cancer (A), muscles (B) and breast cancer (C) using MUSK mouse mAb with DAB staining.)
MUSK, Antibody (Cat# AAA850308)
Full Name
MUSK Antibody
Gene Names
MUSK; CMS9; FADS
Reactivity
Human
Applications
IHC, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot
(Anti-ITGA6 Antibody (isoform 2 S1064) at 1:1000 dilution + SW480 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 119 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
ITGA6, Polyclonal Antibody (Cat# AAA9203054)
Full Name
ITGA6 Antibody (isoform 2 S1064)
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Reactivity
Human (Predicted Reactivity: Chicken)
Applications
WB, IF, IHC-P, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215800)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$210/0.025 mg | $800/5x0.025 mg
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with PI3KCD Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PI3KCD primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PI3KCD immunoreactivity is localized to Cytoplasm significantly.)
PI3KCD, Polyclonal Antibody (Cat# AAA9215071)
Full Name
PI3KCD Antibody (N-term)
Gene Names
PIK3CD; APDS; PI3K; IMD14; p110D; P110DELTA
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
EIA, IHC-p, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Figure 1: Western blot analysis using ATXN1 mAb against HEK293 (1) and ATXN1-hIgGFc transfected HEK293 (2) cell lysate.)
ATXN1, Antibody (Cat# AAA850318)
Full Name
ATXN1 Antibody
Gene Names
ATXN1; ATX1; SCA1; D6S504E
Reactivity
Human
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Figure 1. Western blot analysis using KLHL13 mouse mAb against Hela (1) and MCF-7 (2) cell lysate.)
KLHL13, Antibody (Cat# AAA850920)
Full Name
KLHL13 Antibody
Gene Names
KLHL13; BKLHD2
Reactivity
Human
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(All lanes : Anti-Actl6a Antibody (C-term) at 1/1000 dilutionLane 1: Hela whole cell lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Actl6a, Polyclonal Antibody (Cat# AAA9231934)
Full Name
Mouse Actl6a Antibody (C-term)
Gene Names
Actl6a; ARP4; Actl6; Baf53a; C79802; AI851094; 2810432C06Rik
Reactivity
Human, Mouse
Predicted: Monkey
Predicted: Monkey
Applications
WB, IHC, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(HNRPAB Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HNRPAB antibody detected the HNRPAB protein (arrow).)
HNRPAB, Polyclonal Antibody (Cat# AAA9205445)
Full Name
HNRPAB Antibody (N-term)
Gene Names
HNRNPAB; ABBP1; HNRPAB
Reactivity
Human
Applications
EIA, IHC, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(TSN Antibody (Center) western blot analysis in HL-60,Jurkat,K562 cell line mouse spleen tissue lysates (35ug/lane).This demonstrates the TSN antibody detected the TSN protein (arrow).)
TSN, Polyclonal Antibody (Cat# AAA9214736)
Full Name
TSN Antibody (Center)
Gene Names
TSN; C3PO; RCHF1; TBRBP; TRSLN; BCLF-1; REHF-1
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Hamster)
Applications
EIA, IHC, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from A431,mouse NIH/3T3,rat PC-12 cell line (from left to right), using HSPD1 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
HSPD1, Polyclonal Antibody (Cat# AAA9231721)
Full Name
HSPD1 Antibody (Center)
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse
Predicted: Bovine, Chicken, Hamster, Rat
Predicted: Bovine, Chicken, Hamster, Rat
Applications
WB, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Figure 1: Western blot analysis using AURKA mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate.)
AURKA, Antibody (Cat# AAA850911)
Full Name
AURKA Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human, Monkey, Rat
Applications
WB, IHC, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of lysates from Jurkat,K562 cell line (from left to right), using DHX9 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
DHX9, Polyclonal Antibody (Cat# AAA9231813)
Full Name
DHX9 Antibody (N-term)
Gene Names
DHX9; LKP; RHA; DDX9; NDH2; NDHII
Reactivity
Human
Predicted: Bovine
Predicted: Bovine
Applications
WB, IHC, IF, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
SDS-Page
uCHL5, Active Protein (Cat# AAA204027)
Full Name
uCHL5, 1-329aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
UCHL5; UCH37; CGI-70; INO80R; UCH-L5
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
$215/0.02 mg | $340/0.1 mg | $765/0.5 mg | $3,195/5x0.5 mg
Western Blot (WB)
(Western blot analysis of AOF2 (arrow) using LSD1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).)
LSD1, Polyclonal Antibody (Cat# AAA9212229)
Full Name
LSD1 Antibody (C-term)
Gene Names
KDM1A; AOF2; KDM1; LSD1; BHC110
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, EIA, IHC, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216584)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Biotin
Applications
FC/FACS
Pricing
$210/0.025 mg | $800/5x0.025 mg
Western Blot (WB)
(Figure 1: Western blot analysis using LHX2 mAb against recombinant protein.)
LHX2, Antibody (Cat# AAA850149)
Full Name
LHX2 Antibody
Gene Names
LHX2; LH2; hLhx2
Reactivity
Human
Applications
WB, IHC, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Figure 1. Western blot analysis using PTEN mouse mAb against Hela (1) and NIH/3T3 (2) cell lysate.)
PTEN, Antibody (Cat# AAA850885)
Full Name
PTEN Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Human, Mouse
Applications
WB, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Figure 1. Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.)
beta-Actin, Antibody (Cat# AAA850843)
Full Name
beta-Actin Antibody
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Hamster, Rat, Monkey
Applications
WB, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using PPARA Antibody(green) compared to an isotype control of mouse IgG1(blue). MBS9200027 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
PPARA, Monoclonal Antibody (Cat# AAA9200027)
Full Name
PPARA Antibody
Gene Names
PPARA; PPAR; NR1C1; hPPAR; PPARalpha
Reactivity
Human, mouse
Applications
WB, EIA, FC/FACS, IF, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
( All lanes : Anti-TARDBP Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
TARDBP, Polyclonal Antibody (Cat# AAA9201876)
Full Name
TARDBP Antibody (N-term)
Gene Names
TARDBP; ALS10; TDP-43
Reactivity
Human, Mouse, Rat; Predicted: Chicken
Applications
WB, IF, IHC-P, E/EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot
(Western blot analysis using ABCG2 mouse mAb against HepG2 (1), Cos7 (2), Jurkat (3) and NIH/3T3 (4) cell lysate.)
ABCG2, Monoclonal Antibody (Cat# AAA850721)
Full Name
Mouse Monoclonal Antibody to ABCG2
Gene Names
ABCG2; MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
Applications
EIA, WB, ICC
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis of Hela cells using LPP antibody (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
LPP, Monoclonal Antibody (Cat# AAA5310890)
Full Name
LPP antibody
Applications
EIA, IF, IHC, WB
Pricing
$645/0.1 mL | $2,885/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with FADS2 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.)
FADS2, Polyclonal Antibody (Cat# AAA9210448)
Full Name
FADS2 Antibody (N-term)
Gene Names
FADS2; D6D; DES6; TU13; FADSD6; LLCDL2; SLL0262
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(All lanes : Anti-BRAF Antibody at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: K562 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
BRAF, Monoclonal Antibody (Cat# AAA9217024)
Full Name
BRAF Antibody
Gene Names
BRAF; NS7; BRAF1; RAFB1; B-RAF1
Reactivity
Human
Applications
WB, IF, EIA
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(TBP Antibody (Center) western blot analysis in K562,U251 cell line lysates (35ug/lane).This demonstrates the TBP antibody detected the TBP protein (arrow).)
TBP, Polyclonal Antibody (Cat# AAA9202342)
Full Name
TBP Antibody (Center)
Gene Names
TBP; HDL4; GTF2D; SCA17; TFIID; GTF2D1
Reactivity
Human (Predicted Reactivity: Zebrafish, Xenopus, Bovine, Chicken, Monkey, Mouse)
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215789)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$525/0.25 mg | $2,210/5x0.25 mg
Western Blot (WB)
(Figure 1: Western blot analysis using WNT5A mouse mAb against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2). Figure 1: Western blot Figure 1:)
WNT5A, Antibody (Cat# AAA850867)
Full Name
WNT5A Antibody
Gene Names
WNT5A; hWNT5A
Reactivity
Human
Applications
WB, IHC, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
