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Western Blot (WB) (Western blot analysis using GUCY1A3 mouse mAb against HEK293 (1) and Raji (2) cell lysate.)

Mouse anti-Human GUCY1A3 Monoclonal Antibody | anti-GUCY1A3 antibody

Anti-GUCY1A3 Mouse mAb

Gene Names
GUCY1A3; GUCA3; MYMY6; GC-SA3; GUC1A3; GUCSA3; GUCY1A1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
GUCY1A3; Monoclonal Antibody; Anti-GUCY1A3 Mouse mAb; GUCA3; GC-SA3; GUC1A3; GUCSA3; GUCY1A1; anti-GUCY1A3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3G6B2
Form/Format
Purified antibody in PBS with 0.05% sodium azide.
Sequence Length
690
Applicable Applications for anti-GUCY1A3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
Immunogen
Purified recombinant fragment of human GUCY1A3 (AA: 22-214) expressed in E Coli.

Western Blot (WB)

(Western blot analysis using GUCY1A3 mouse mAb against HEK293 (1) and Raji (2) cell lysate.)

Western Blot (WB) (Western blot analysis using GUCY1A3 mouse mAb against HEK293 (1) and Raji (2) cell lysate.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HepG2 cells using GUCY1A3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.)

Immunofluorescence (IF) (Immunofluorescence analysis of HepG2 cells using GUCY1A3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded renal cancer tissues using GUCY1A3 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded renal cancer tissues using GUCY1A3 mouse mAb with DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HEK293 cells using GUCY1A3 mouse mAb (green) and negative control (purple).)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of HEK293 cells using GUCY1A3 mouse mAb (green) and negative control (purple).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GUCY1A3 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GUCY1A3 mouse mAb with DAB staining.)
Related Product Information for anti-GUCY1A3 antibody
Entrez Summary: Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3', 5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
77.5
NCBI Official Full Name
guanylate cyclase soluble subunit alpha-3 isoform A
NCBI Official Synonym Full Names
guanylate cyclase 1 soluble subunit alpha
NCBI Official Symbol
GUCY1A3
NCBI Official Synonym Symbols
GUCA3; MYMY6; GC-SA3; GUC1A3; GUCSA3; GUCY1A1
NCBI Protein Information
guanylate cyclase soluble subunit alpha-3
UniProt Protein Name
Guanylate cyclase soluble subunit alpha-3
Protein Family
UniProt Gene Name
GUCY1A3
UniProt Synonym Gene Names
GUC1A3; GUCSA3; GUCY1A1; GCS-alpha-3

NCBI Description

Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

GUCY1A3: Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Protein type: EC 4.6.1.2; Guanylyl cyclase; Lyase; Nucleotide Metabolism - purine; Receptor, misc.

Chromosomal Location of Human Ortholog: 4q32.1

Cellular Component: guanylate cyclase complex, soluble; plasma membrane

Molecular Function: adenylate cyclase activity; guanylate cyclase activity; protein binding; receptor activity

Biological Process: blood circulation; cGMP biosynthetic process; nitric oxide mediated signal transduction

Disease: Moyamoya Disease 6 With Achalasia

Research Articles on GUCY1A3

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Product Notes

The GUCY1A3 gucy1a3 (Catalog #AAA476665) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-GUCY1A3 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GUCY1A3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1/500 - 1/2000 ICC: 1/200 - 1/1000 IHC: 1/200 - 1/1000 FC: 1/200 - 1/400. Researchers should empirically determine the suitability of the GUCY1A3 gucy1a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GUCY1A3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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