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6044 results for " Fluorescent" - showing 400-450
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. small intestine section using MGMT Antibody. MBS9200128 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
MGMT, Monoclonal Antibody (Cat# AAA9200128)
Full Name
MGMT Antibody
Reactivity
Human
Applications
EIA, IHC, IF, WB
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Figure 1: Western blot analysis using AKT2 mouse mAb against A431 (1), Jurkat (2), HEK293 (3), A549 (4), MCF-7 (5) and PC-12 (6) cell lysate.)
AKT2, Antibody (Cat# AAA850234)
Full Name
AKT2 Antibody
Gene Names
AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Reactivity
Human, Rat, Monkey
Applications
WB, IHC, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Figure 1: Western blot analysis using CHUK mouse mAb against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.)
CHUK, Antibody (Cat# AAA850370)
Full Name
CHUK Antibody
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human
Applications
WB, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of lysates from Ramos,Daudi cell line (from left to right),using PTK2B Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
PTK2B, Polyclonal Antibody (Cat# AAA9231596)
Full Name
PTK2B Antibody (C-term)
Gene Names
PTK2B; PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
WB, IHC, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. stomach section using GSS Antibody (C-term). MBS9214860 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
GSS, Polyclonal Antibody (Cat# AAA9214860)
Full Name
GSS Antibody (C-term)
Gene Names
GSS; GSHS; HEL-S-64p; HEL-S-88n
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Applications
EIA, IHC, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from A431, Hela, HepG2 cell line, mouse brain tissue and MCF-7, U-251 MG cell line (from left to right), using CYC1 Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
CYC1, Polyclonal Antibody (Cat# AAA9231622)
Full Name
CYC1 Antibody (Center)
Gene Names
CYC1; UQCR4; MC3DN6
Reactivity
Human, Mouse
Applications
WB, IHC, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-STRADA Antibody (C-term) at 1:1000 dilutionLane 1: 293T whole cell lysatesLane 2: human brain lysatesLane 3: mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
STRADA, Polyclonal Antibody (Cat# AAA9231944)
Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96; STRAD alpha
Reactivity
Human, Mouse
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent confocal image of U251 cell stained with EWSR1 Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly.)
EWSR1, Polyclonal Antibody (Cat# AAA9201155)
Full Name
EWSR1 Antibody (C-term)
Gene Names
EWSR1; EWS; EWS-FLI1; bK984G1.4
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
IF, EIA, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with CDKN1A Antibody (C-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1A primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).CDKN1A immunoreactivity is localized to Nucleus significantly.)
CDKN1A, Polyclonal Antibody (Cat# AAA9208019)
Full Name
CDKN1A Antibody (C-term)
Gene Names
CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Reactivity
Human, mouse
Applications
EIA, IHC, WB, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody (N-term). MBS9208816 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
CA2, Polyclonal Antibody (Cat# AAA9208816)
Full Name
CA2 Antibody (N-term)
Gene Names
CA2; CAC; CAII; Car2; CA-II; HEL-76
Reactivity
Human, mouse
Applications
IF, EIA, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(All lanes : Anti-NPM1 Antibody (N-term) at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NPM1, Polyclonal Antibody (Cat# AAA9232006)
Full Name
NPM1 Antibody (N-term)
Gene Names
NPM1; B23; NPM
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG4D (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9211764 ATG4D (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG4D immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG4D, Polyclonal Antibody (Cat# AAA9211764)
Full Name
ATG4D Antibody (C-term)
Gene Names
ATG4D; APG4D; AUTL4; APG4-D
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG4D (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9210910 ATG4D (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D (N-term) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG4D, Polyclonal Antibody (Cat# AAA9210910)
Full Name
ATG4D Antibody (N-term)
Gene Names
ATG4D; APG4D; AUTL4; APG4-D
Reactivity
Mouse
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9201599 FASN (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the FASN mainly to the mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710).)
FASN, Polyclonal Antibody (Cat# AAA9201599)
Full Name
FASN Antibody (Center)
Gene Names
FASN; FAS; OA-519; SDR27X1
Reactivity
Human
Applications
IF, EIA, WB, IHC, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215799)
Full Name
RAT ANTI MOUSE DECTIN-1:RPE
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$255/25 Tests | $995/5x25 Tests
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216593)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
FC/FACS
Pricing
$550/100 Tests | $2,320/5x100 Tests
Western Blot (WB)
(Western blot analysis of SOX4 antibody (N-term) in A2058 cell line lysates (35ug/lane). SOX4 (arrow) was detected using the purified Pab.)
SOX4, Polyclonal Antibody (Cat# AAA9211626)
Full Name
SOX4 Antibody (N-term)
Gene Names
SOX4; EVI16
Reactivity
Human
Applications
IF, EIA, WB, IHC-p, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Lin28-TAT, Active Protein (Cat# AAA691617)
Full Name
Human Lin28-TAT
Gene Names
LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
Reactivity
Human
Purity
> 90% by SDS-PAGE & HPLC analyses
Pricing
$395/0.025 mg | $250/0.005 mg | $1,565/5x0.025 mg
Western Blot (WB)
(Western blot analysis of lysates from HT1080,ZR-75-1 cell line (from left to right), using CRTAP Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
CRTAP, Polyclonal Antibody (Cat# AAA9231673)
Full Name
CRTAP Antibody (N-term)
Gene Names
CRTAP; OI7; CASP; P3H5; LEPREL3
Reactivity
Human
Predicted: Mouse
Predicted: Mouse
Applications
WB, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from K562 cell line,mouse stomach tissue lysate(from left to right), using BLMH Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
BLMH, Polyclonal Antibody (Cat# AAA9231778)
Full Name
BLMH Antibody (Center)
Gene Names
BLMH; BH; BMH
Reactivity
Human, Mouse
Predicted: Rabbit, Rat
Predicted: Rabbit, Rat
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded M. brain section using Mouse Sirt1 Antibody (C-term). MBS9210986 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Sirt1, Polyclonal Antibody (Cat# AAA9210986)
Full Name
Mouse Sirt1 Antibody (C-term)
Gene Names
Sirt1; Sir2; Sir2a; SIR2L1; AA673258; Sir2alpha
Reactivity
Mouse
Applications
EIA, IHC, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western Blot showing CTNNB1 antibody used against CTNNB1-hIgGFc transfected HEK293 cell lysate.)
CTNNB1, Monoclonal Antibody (Cat# AAA533953)
Full Name
CTNNB1 antibody
Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Applications
EIA, FC/FACS, IF, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Immunohistochemistry (IHC)
(Anti-SCAP antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
SCAP, Polyclonal Antibody (Cat# AAA248222)
Full Name
Rabbit Polyclonal to Human SCAP
Reactivity
Human
Applications
IHC - Paraffin, IF, WB, FC/FACS
Purity
Immunoaffinity Purified
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysate from HT1080 cell line,using IFITM3 Antibody (N-term). MBS9201518 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.)
Interferon-inducible protein (IFITM3), Polyclonal Antibody (Cat# AAA9201518)
Full Name
Interferon-inducible protein (IFITM3) Antibody (N-term)
Gene Names
IFITM3; 1-8U; IP15; DSPA2b
Reactivity
Human
Applications
WB, EIA, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Anti-SOX2 Antibody (C265) at 1:1000 dilution + NCCIT whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SOX2, Polyclonal Antibody (Cat# AAA9217117)
Full Name
SOX2 Antibody (C265)
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Human; Predicted Reactivity: Chicken, Mouse, Xenopus
Applications
WB, IF, FC/FACS, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215790)
Full Name
RAT ANTI MOUSE DECTIN-1:Biotin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis using BLK mAb against HEK293 (1) and BLK (AA)
BLK, Monoclonal Antibody (Cat# AAA476100)
Full Name
Anti-BLK Mouse mAb
Reactivity
Human
Applications
WB, ICC, FC/FACS
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from HepG2, MCF-7, A431 cell line (from left to right), using CTSD Antibody. It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)
CTSD, Polyclonal Antibody (Cat# AAA9231589)
Full Name
CTSD Antibody
Gene Names
CTSD; CPSD; CLN10; HEL-S-130P
Reactivity
Human
Applications
WB, IHC, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC)
(Anti-TSHZ2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
TSHZ2, Polyclonal Antibody (Cat# AAA248786)
Full Name
Rabbit Polyclonal to Human TSHZ2
Reactivity
Human
Applications
IHC - Paraffin, IF, WB, FC/FACS
Purity
Immunoaffinity Purified
Pricing
$610/0.2 mL | $2,685/5x0.2 mL
Western Blot (WB)
(All lanes : Anti-Ctr9 Antibody (C-term) at 1:1000 dilutionLane 1: HT-29 whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: mouse cerebellum lysatesLane 4: mouse testis lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 133 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Ctr9, Polyclonal Antibody (Cat# AAA9231990)
Full Name
Mouse Ctr9 Antibody (C-term)
Gene Names
Ctr9; Tsp; Tsbp; Sh2bp1; AA409336; mKIAA0155
Reactivity
Human, Mouse
Applications
WB, IHC, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (C-term). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).AKT2 immunoreactivity is localized to Cytoplasm significantly.)
AKT2, Polyclonal Antibody (Cat# AAA9202626)
Full Name
AKT2 Antibody (C-term)
Gene Names
AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(All lanes : Anti-KLF9 Antibody (N-term) at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
KLF9, Polyclonal Antibody (Cat# AAA9212765)
Full Name
KLF9 Antibody (N-term)
Gene Names
KLF9; BTEB; BTEB1
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Applications
WB, EIA, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF)
(Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye)
BRAF, Monoclonal Antibody (Cat# AAA5310888)
Full Name
BRAF antibody
Applications
EIA, IF, IHC, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Western Blot (WB)
(Figure 1: Western blot analysis using CSK mouse mAb against NIH/3T3 (1), Hela (2), COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.)
CSK, Antibody (Cat# AAA850712)
Full Name
CSK Antibody
Reactivity
Human, Mouse, Monkey, Rat
Applications
WB, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(All lanes : Anti-Smarcc1 Antibody (C-term) at 1:1000 dilutionLane 1: C2C12 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 123 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Smarcc1, Polyclonal Antibody (Cat# AAA9231978)
Full Name
(Mouse) Smarcc1 Antibody (C-term)
Gene Names
Smarcc1; Rsc8; SRG3; msp3; BAF155; AI115498
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
WB, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(APRT Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the APRT antibody detected the APRT protein (arrow).)
APRT, Polyclonal Antibody (Cat# AAA9201046)
Full Name
APRT Antibody (N-term)
Gene Names
APRT; AMP; APRTD
Reactivity
Human
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215794)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$280/0.05 mg | $1,115/5x0.05 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215796)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$210/0.025 mg | $800/5x0.025 mg
Western Blot (WB)
(Western blot analysis of lysates from human skeletal muscle and mouse brain tissue lysate (from left to right), using CFL2 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
CFL2, Polyclonal Antibody (Cat# AAA9231616)
Full Name
CFL2 Antibody (C-term)
Gene Names
CFL2; NEM7
Reactivity
Human, Mouse
Predicted: Bovine, Chicken, Pig, Xenopus
Predicted: Bovine, Chicken, Pig, Xenopus
Applications
WB, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from human heart tissue, HepG2, PC-3, Ramos cell line (from left to right), using NDUFB4 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
NDUFB4, Polyclonal Antibody (Cat# AAA9231612)
Full Name
NDUFB4 Antibody (N-term)
Gene Names
NDUFB4; B15; CI-B15
Reactivity
Human
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from Hela,mouse F9 cell line,mouse brain tissue lysate,K562 cell line,rat brain tissue lysate (from left to right), using COPS2 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
COPS2, Polyclonal Antibody (Cat# AAA9231785)
Full Name
COPS2 Antibody (N-term)
Gene Names
COPS2; CSN2; SGN2; ALIEN; TRIP15
Reactivity
Human, Mouse, Rat
Predicted: Zebrafish, Xenopus
Predicted: Zebrafish, Xenopus
Applications
WB, IHC, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
APRT, Polyclonal Antibody (Cat# AAA9232055)
Full Name
APRT Antibody (N-term)
Gene Names
APRT; AMP; APRTD
Reactivity
Human
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from HepG2,Hela,A431,mouse NIH/3T3 cell line,rat brain tissue lysate(from left to right), using TUFM Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
TUFM, Polyclonal Antibody (Cat# AAA9231791)
Full Name
TUFM Antibody (N-term)
Gene Names
TUFM; P43; EFTU; COXPD4; EF-TuMT
Reactivity
Human, Mouse, Rat
Predicted: Bovine
Predicted: Bovine
Applications
WB, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from WiDr,Jurkat,mouse NIH/3T3,rat PC-12,Hela cell line (from left to right), using ATP5B Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
ATP5B, Polyclonal Antibody (Cat# AAA9231807)
Full Name
ATP5B Antibody (Center)
Gene Names
ATP5F1B; ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human, Mouse, Rat
Predicted: Bovine
Predicted: Bovine
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(CNOT8 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CNOT8 antibody detected the CNOT8 protein (arrow).)
CNOT8, Polyclonal Antibody (Cat# AAA9201317)
Full Name
CNOT8 Antibody (C-term)
Gene Names
CNOT8; CAF1; POP2; CALIF; Caf1b; hCAF1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216590)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN
Applications
FC/FACS, IF, WB
Pricing
$525/0.25 mg | $2,210/5x0.25 mg
Western Blot (WB)
(Western blot analysis of lysates from A431,K562 cell line (from left to right), using PTGS1 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
PTGS1, Polyclonal Antibody (Cat# AAA9231848)
Full Name
PTGS1 Antibody (C-term)
Gene Names
PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
Reactivity
Human
Applications
WB, IHC, IF, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Figure 1: Western blot analysis using MATN1 mAb against HEK293 (1) and MATN1-hIgGFc transfected HEK293 (2) cell lysate.)
MATN1, Antibody (Cat# AAA850796)
Full Name
MATN1 Antibody
Gene Names
MAT1A; MAT; SAMS; MATA1; SAMS1
Reactivity
Human
Applications
WB, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Flow Cytometry (FC/FACS)
(FC analysis of K562 cells using COX4I1 antibody (blue) and negative control (red).)
COX4I1, Monoclonal Antibody (Cat# AAA5310901)
Full Name
COX4I1 antibody
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Applications
EIA, IF, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from Hela cell line,untreated or treated with FBS and calyculin A,using Phospho-CDC25A(T507) Antibody(upper) or Beta-actin (lower).)
CDC25A, Polyclonal Antibody (Cat# AAA9232009)
Full Name
Phospho-CDC25A (T507) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human
Predicted: Bovine, Mouse, Rat
Predicted: Bovine, Mouse, Rat
Applications
WB, IF, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
