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1917 results for " Interleukin 15" - showing 200-250
Western Blot (WB)
(Western blot analysis of Caspase-1 in HeLa cell lysate with Caspase-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 μg/mL.)
Caspase-1, Polyclonal Antibody (Cat# AAA150194)
Full Name
Caspase-1 Antibody
Gene Names
CASP1; ICE; P45; IL1BC
Reactivity
Human
Applications
EIA, WB, IHC, IF
Purity
Caspase-1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Activity Data
(Figure. The binding activity of IL8 with SDC1.Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cellsendothelial cells. IL-8, also known as neutrophil chemotactic factor, has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL8 also induces phagocytosis once they have arrived. IL8 is also known to be a potent promoter of angiogenesis. Besides, Syndecan 1 (SDC1) has been identified as an interactor of IL8, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL8 and recombinant human SDC1. Briefly, IL8 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SDC1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL8 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL8 and SDC1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 8 (IL8), Active Protein (Cat# AAA2105072)
Full Name
Active Interleukin 8 (IL8)
Gene Names
CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Western Blot (WB)
(Western blot analysis of TIRAP in human heart tissue lysate with TIRAP antibody at (A) 1 and (B) 2 μg/mL.)
TIRAP, Polyclonal Antibody (Cat# AAA151549)
Full Name
TIRAP Antibody
Gene Names
TIRAP; Mal; wyatt; BACTS1; MyD88-2
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
TIRAP Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Western blot analysis of IL15 using A549 whole lysates.)
IL15, Polyclonal Antibody (Cat# AAA9608241)
Full Name
IL15 Antibody
Gene Names
IL15; IL-15
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-IL21 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IL21, Polyclonal Antibody (Cat# AAA241280)
Full Name
Rabbit Polyclonal to Human IL21
Gene Names
IL21; Za11; IL-21; CVID11
Reactivity
Human
Applications
IHC - Paraffin, WB
Purity
Immunoaffinity Purified
Pricing
$705/0.05 mg | $3,120/5x0.05 mg
Immunohistochemistry (IHC)
(Anti-IL-32 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IL32, Polyclonal Antibody (Cat# AAA240344)
Full Name
Rabbit Polyclonal to Human IL32
Gene Names
IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
Reactivity
Human, Mouse
Applications
IHC - Paraffin, WB
Purity
Immunoaffinity Purified
Pricing
$610/0.05 mg | $2,685/5x0.05 mg
Typical Testing Data/Standard Curve (for reference only)
Interleukin 15, IL-15, ELISA Kit (Cat# AAA164192)
Full Name
Porcine Interleukin 15, IL-15 ELISA Kit
Reactivity
Porcine
Pricing
$365/48-Strip-Wells | $505/96-Strip-Wells | $2,115/5x96-Strip-Wells | $4,170/10x96-Strip-Wells
Activity Data
(IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Besides, mouse IL1B shares 87.0% AA sequence identity with rat IL1B, suggesting the exist of cross-species activity. Thus, a binding ELISA assay was constructed to detect the association of recombinant rat IL1R1 with recombinant mouse IL1B. Briefly, IL1R1 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1B-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1B was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 1 Receptor Type I (IL1R1), Active Protein (Cat# AAA2097340)
Full Name
Active Interleukin 1 Receptor Type I (IL1R1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$160/0.01 mg | $300/0.05 mg | $460/0.1 mg | $565/0.2 mg | $1,080/0.5 mg | $1,600/1 mg | $3,920/5 mg
Interleukin-15, Active Protein (Cat# AAA143557)
Full Name
Recombinant Mouse Interleukin-15, His Tag
Gene Names
Il15; IL-15; AI503618
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing
$250/0.01 mg | $6,100/1 mg | $180/0.002 mg | $27,220/5x1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of IL15RA using anti-IL15RA antibody (MBS1750809).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL15RA antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL15RA at approximately 28KD. The expected band size for IL15RA is at 28KD. )
IL15RA, Polyclonal Antibody (Cat# AAA1750809)
Full Name
Anti-IL15RA Picoband Antibody
Gene Names
IL15RA; CD215
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Activity Data
(Figure. The binding activity of IL18 with CASP1.Interleukin-18 (IL18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. IL18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). Besides, Caspase 1 (CASP1) has been identified as an interactor of IL18, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL18 and recombinant human CASP1. Briefly, IL18 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CASP1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL18 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL18 and CASP1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 18 (IL18), Active Protein (Cat# AAA2105068)
Full Name
Active Interleukin 18 (IL18)
Gene Names
IL18; IGIF; IL-18; IL-1g; IL1F4
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$190/0.01 mg | $390/0.05 mg | $595/0.1 mg | $735/0.2 mg | $1,420/0.5 mg | $2,115/1 mg | $5,200/5 mg
Activity Data
(IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Besides, mouse IL1B shares 87.0% AA sequence identity with rat IL1B, suggesting the exist of cross-species activity. Thus, a binding ELISA assay was constructed to detect the association of recombinant rat IL1R1 with recombinant mouse IL1B. Briefly, IL1R1 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1B-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1B was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 1 Receptor Type I (IL1R1), Active Protein (Cat# AAA2097339)
Full Name
Active Interleukin 1 Receptor Type I (IL1R1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$160/0.01 mg | $300/0.05 mg | $460/0.1 mg | $565/0.2 mg | $1,080/0.5 mg | $1,600/1 mg | $3,920/5 mg
Activity Data
(IL17D (Interleukin-17D) belongs to the IL17 (interleukin 17) family, which includes six members (IL-17, IL-17B through IL-17F). IL17D is reported to induce the expression of IL6, CXCL8/IL8, and CSF2/GM-CSF from endothelial cells. IL17 family members share some similarities in AA sequence and structure, thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17D with recombinant human IL17RB. Briefly, IL17D were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RB-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17D pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17D with IL17RB was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 17D (IL17D), Active Protein (Cat# AAA2097331)
Full Name
Active Interleukin 17D (IL17D)
Gene Names
IL17D; IL-17D
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Western Blot (WB)
(Western Blot analysis of HepG2 cell using IL2RB Polyclonal Antibody at dilution of 1:650)
IL2RB, Polyclonal Antibody (Cat# AAA2523370)
Full Name
IL2RB Polyclonal Antibody
Gene Names
IL2RB; CD122; IL15RB; P70-75
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Affinity Purification
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB)
(Western blot analysis IL9 using COLO205 whole cell lysates)
IL9, Polyclonal Antibody (Cat# AAA9608798)
Full Name
IL9 Antibody
Gene Names
IL9; P40; HP40; IL-9
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Interleukin 15, Active Protein (Cat# AAA650118)
Full Name
Interleukin 15, Recombinant, Mouse (IL-15)
Gene Names
IL15; IL-15; MGC9721
Purity
Highly Purified
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Pricing
$515/0.01 mg | $365/0.002 mg | $2,305/5x0.01 mg
Western Blot (WB)
(Western blot analysis of extracts of K-562 cells, using IL15RA antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IL15RA, Polyclonal Antibody (Cat# AAA126709)
Full Name
IL15RA
Gene Names
IL15RA; CD215
Reactivity
Human
Applications
WB, IF, ICC
Purity
Affininty Purification
Pricing
$285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-TLR5 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
TLR5, Polyclonal Antibody (Cat# AAA241332)
Full Name
Rabbit Polyclonal to Human TLR5
Gene Names
TLR5; SLE1; TIL3; SLEB1; MELIOS
Reactivity
Human, Mouse
Applications
IHC - Paraffin, ICC, WB
Purity
Affinity Purified
Pricing
$585/0.05 mg | $2,585/5x0.05 mg
Typical Testing Data/Standard Curve
(Human IL-37/IL1F7/F1L1Z ELISA Kit)
IL-37/IL1F7/F1L1Z, ELISA Kit (Cat# AAA9135824)
Full Name
Human IL-37/IL1F7/F1L1Z ELISA Kit
Gene Names
IL37; FIL1; FIL1Z; IL-1H; IL-37; IL1F7; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA)
Reactivity
Human
Pricing
$555/96 Tests
SDS-Page
(15% SDS-PAGE (3ug))
Interleukin 4, 25-153 aa, Active Protein (Cat# AAA638274)
Full Name
Interleukin 4, 25-153 aa, Recombinant, Human (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MG
Purity
Highly Purified
95% by SDS-PAGE
95% by SDS-PAGE
Pricing
$740/0.05 mg | $3,325/5x0.05 mg
Interleukin-4, Active Protein (Cat# AAA546165)
Full Name
Recombinant Human Interleukin-4 (rHu IL-4)
Gene Names
IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
Purity
>95% by SDS-PAGE and HPLC analyses.
Pricing
$285/0.02 mg | $3,695/1 mg | $210/0.005 mg | $16,410/5x1 mg
Activity Data
(IL17RC (Interleukin-17 receptor C) is a receptor for IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RA. This protein is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F with similar affinities. A binding ELISA assay was conducted to detect the association of IL17RC with IL17RA. Briefly, IL17RC were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17RC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RC with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 17 Receptor C (IL17RC), Active Protein (Cat# AAA2097219)
Full Name
Active Interleukin 17 Receptor C (IL17RC)
Gene Names
IL17RC; CANDF9; IL17RL; IL17-RL
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Immunofluorescence (IF)
(MBS9606161 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IL15RA, Polyclonal Antibody (Cat# AAA9606161)
Full Name
IL15RA Antibody
Gene Names
IL15RA; CD215
Reactivity
Human, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Interleukin-15, Active Protein (Cat# AAA146739)
Full Name
Recombinant Human Interleukin-15, HEK
Gene Names
IL15; IL-15
Purity
Greater than 95.0% as determined by SDS-PAGE.
Typical Testing Data/Standard Curve
(Human IL-25/IL-17E ELISA Kit)
IL-25/IL-17E, ELISA Kit (Cat# AAA9135878)
Full Name
Human IL-25/IL-17E ELISA Kit
Gene Names
IL25; IL17E
Reactivity
Human
Pricing
$555/96 Tests
Activity Data
(Interleukin 15 (IL15) is a widely expressed cytokine that is structurally and functionally related to IL2, which plays an important role in many immunological diseases. IL15 also regulates T and natural killer (NK) cell activation and proliferation. To test the effect of IL15 on cells proliferation of human T lymphocyte cells, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL15. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of Jurkat cells after incubation with IL15 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of IL15 was shown in Figure 2. It was obvious that IL15 significantly promoted cell proliferation of Jurkat cells. The ED50 for this effect is typically 0.7240 to 5.206ng/mL.)
Interleukin 15 (IL15), Active Protein (Cat# AAA2097226)
Full Name
Active Interleukin 15 (IL15)
Gene Names
IL15; IL-15
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$275/0.01 mg | $620/0.05 mg | $970/0.1 mg | $1,195/0.2 mg | $2,355/0.5 mg | $3,490/1 mg | $8,480/5 mg
Typical Testing Data/Standard Curve
(Mouse IL-25/IL-17E ELISA Kit)
IL-25/IL-17E, ELISA Kit (Cat# AAA9135766)
Full Name
Mouse IL-25/IL-17E ELISA Kit
Gene Names
IL25; IL17E
Reactivity
Mouse
Pricing
$555/96 Tests
Activity Data
(IL17RD (interleukin-17 receptor D) is a membrane protein belonging to the IL17R protein family, acting as a component of the interleukin-17 receptor signaling complex. Knowing that the interaction between this protein and IL-17R does not require the interleukin, we have conducted a binding a binding ELISA assay to detect the interaction of recombinant human IL17RD with both recombinant human IL17RA and IL17. Briefly, IL17RD were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated or IL17-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb and anti-IL17 pAb separately, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RD with IL17RA and IL17 was shown in Figure 1 and Figure 2 respectively and this effect was in a dose dependent manner.)
Interleukin 17 Receptor D (IL17RD), Active Protein (Cat# AAA2097349)
Full Name
Active Interleukin 17 Receptor D (IL17RD)
Gene Names
IL17RD; SEF; HH18; IL-17RD; IL17RLM
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$250/0.01 mg | $550/0.05 mg | $855/0.1 mg | $1,055/0.2 mg | $2,075/0.5 mg | $3,075/1 mg | $7,525/5 mg
Activity Data
(Figure. The binding activity of IL4 with IL2Rg.The interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL12. Besides, Interleukin 2 Receptor Gamma (IL2Rg) has been identified as an interactor of IL4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat IL4 and recombinant rat IL2Rg. Briefly, IL4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL2Rg-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL4 and IL2Rg was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 4 (IL4), Active Protein (Cat# AAA2105071)
Full Name
Active Interleukin 4 (IL4)
Gene Names
Il4; Il4e12
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$240/0.01 mg | $515/0.05 mg | $795/0.1 mg | $985/0.2 mg | $1,920/0.5 mg | $2,845/1 mg | $6,960/5 mg
Activity Data
(IL17 (interleukin 17) is a member of IL17 cytokine family, which is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. It is reported that IL17 is a ligand for IL17RA, indicating its interaction with IL17RA. Thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17 with recombinant human IL17RA. Briefly, IL17 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17 with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.)
Interleukin 17 (IL17), Active Protein (Cat# AAA2097328)
Full Name
Active Interleukin 17 (IL17)
Gene Names
IL17A; IL17; CTLA8; IL-17; CTLA-8; IL-17A
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$155/0.01 mg | $285/0.05 mg | $430/0.1 mg | $525/0.2 mg | $1,010/0.5 mg | $1,490/1 mg | $3,645/5 mg | $16,390/5x5 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IL2RB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)
IL2RB, Polyclonal Antibody (Cat# AAA9127279)
Full Name
IL2RB Polyclonal Antibody
Gene Names
IL2RB; CD122; IL15RB; P70-75
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Interleukin-15 Receptor Subunit alpha (Il15ra), Active Protein (Cat# AAA7115077)
Full Name
Recombinant Mouse Interleukin-15 Receptor Subunit alpha (Il15ra), Partial
Gene Names
Il15ra; IL-15RA; AA690181
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$165/0.01 mg | $250/0.05 mg | $1,005/5x0.05 mg
SDS-Page
(Recombinant Human IL18R1/CD218a Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 80-105 kDa.)
IL18R1/CD218a, Active Protein (Cat# AAA9139694)
Full Name
Recombinant Human IL18R1/CD218a Protein
Gene Names
IL18R1; CD218a; IL18RA; IL1RRP; CDw218a; IL-1Rrp; IL-18Ralpha; IL18Ralpha2; IL-18R-alpha
Purity
>85% by SDS-PAGE.
Pricing
$155/0.02 mg | $185/0.05 mg | $240/0.1 mg | $310/0.2 mg | $545/0.5 mg | $2,295/5x0.5 mg
Western Blot (WB)
(Western blot analysis of RICK in (A) 3T3 and (B) K562 cell lysate with RICK antibody at 0.5 μg/mL)
RICK, Polyclonal Antibody (Cat# AAA151470)
Full Name
RICK Antibody
Gene Names
RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
Reactivity
Human
Applications
EIA, WB, ICC, IF
Purity
RICK Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-IL-18 antibody SAIC-24B-4 Human peripheral blood monocytes (PBMC’s) were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of SAIC-24B-4 (MBS488409, blue line) at a dilution of 1:100 for 1h at RT. An additional population of PBMC’s stimulated with PMA and Ionomycin in the presence of Monensin for 4 hours were stained in a similar manner (purple line). After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IL-18, Monoclonal Recombinant Antibody (Cat# AAA488409)
Full Name
Anti-IL-18 [SAIC-24B-4]
Gene Names
IL18; IGIF; IL-18; IL-1g; IL1F4
Reactivity
Human
Applications
Immuno-MRM
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Lungkine (CXCL15), Active Protein (Cat# AAA692121)
Full Name
Mouse Lungkine (CXCL15)
Gene Names
Cxcl15; Il8; weche; Scyb15; lungkine
Reactivity
Mouse
Purity
> 98% by SDS-PAGE
Pricing
$295/0.02 mg | $145/0.005 mg | $1,270/5x0.02 mg
Interleukin 15, Active Protein (Cat# AAA650183)
Full Name
Interleukin 15, Recombinant, Human (IL-15)
Gene Names
IL15; IL-15; MGC9721
Purity
Highly Purified
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Pricing
$445/0.01 mg | $365/0.002 mg | $1,985/5x0.01 mg
Interleukin-15 (IL15), Active Protein (Cat# AAA7115036)
Full Name
Recombinant Human Interleukin-15 (IL15)
Gene Names
IL15; IL-15
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$300/0.01 mg | $710/0.05 mg | $3,035/5x0.05 mg
Immunohistochemistry (IHC)
(MBS9604035 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IL15, Polyclonal Antibody (Cat# AAA9604035)
Full Name
IL15 Antibody
Gene Names
IL15; IL-15
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(The interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL12. Besides, Interleukin 2 Receptor Gamma (IL2Rg) has been identified as an interactor of IL4, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IL4 and recombinant mouse IL2Rg. Briefly, IL4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to IL2Rg-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL4 and IL2Rg was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of IL4 with IL2Rg.)
Interleukin 4 (IL4), Active Protein (Cat# AAA2105070)
Full Name
Active Interleukin 4 (IL4)
Gene Names
Il4; Il-4; BSF-1
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$195/0.01 mg | $400/0.05 mg | $615/0.1 mg | $755/0.2 mg | $1,460/0.5 mg | $2,175/1 mg | $5,305/5 mg
Activity Data
(Figure. The binding activity of IL33 with IL1RL1.Interleukin 33 is a member of the IL-1 family that potently drives production of T helper-2 (Th2)-associated cytokines. IL33 is a ligand for ST2 (IL1RL1), an IL-1 family receptor that is highly expressed on Th2 cells, mast cells and group 2 innate lymphocytes. IL-33 is expressed by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. Besides, Interleukin 1 Receptor Like Protein 1 (IL1RL1) has been identified as an interactor of IL33, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL33 and recombinant human IL1RL1. Briefly, IL33 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1RL1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL33pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL33and IL1RL1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 33 (IL33), Active Protein (Cat# AAA2105157)
Full Name
Active Interleukin 33 (IL33)
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$240/0.01 mg | $515/0.05 mg | $800/0.1 mg | $990/0.2 mg | $1,935/0.5 mg | $2,865/1 mg | $7,010/5 mg
Activity Data
(Figure. The binding activity of IL28B with IL10Rb.Interleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 28B (IL28B), Active Protein (Cat# AAA2105159)
Full Name
Active Interleukin 28B (IL28B)
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
SDS-Page
(Recombinant Human IL-18 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18 kDa.)
IL-18, Active Protein (Cat# AAA9139609)
Full Name
Recombinant Human IL-18 Protein
Gene Names
IL18; IGIF; IL-18; IL-1g; IL1F4
Purity
>95% by SDS-PAGE.
Pricing
$155/0.01 mg | $180/0.02 mg | $225/0.05 mg | $305/0.1 mg | $425/0.2 mg | $1,770/5x0.2 mg
IL15, Active Protein (Cat# AAA553664)
Full Name
IL15
Gene Names
IL15; IL-15
Applications
SDS-PAGE
Purity
>95%, as determined by SDS-PAGE and HPLC.
Pricing
$290/0.01 mg | $4,005/1 mg | $210/0.002 mg
SDS-Page
(15% SDS-PAGE)
Interleukin 2, Active Protein (Cat# AAA650242)
Full Name
Interleukin 2, Recombinant, Human (Interleukin-2, IL-2, IL2, Aldesleukin, Lymphokine, T-cell Growth Factor, TCGF)
Gene Names
IL2; IL-2; TCGF; lymphokine
Purity
Highly Purified
95% by SDS PAGE
95% by SDS PAGE
Pricing
$600/0.1 mg | $2,695/5x0.1 mg
Activity Data
(IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to play a crucial role in innate immunity of the epithelium. IL17C binds with affinity to IL17RA. Thus, a binding ELISA assay was constructed to detect the association of rhIL17C with IL17RA. Briefly, rhIL17C were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.Figure. The binding activity of IL17C with IL17RA.)
Interleukin 17C (IL17C), Active Protein (Cat# AAA2097330)
Full Name
Active Interleukin 17C (IL17C)
Gene Names
IL17C; CX2; IL-17C
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Typical Testing Data/Standard Curve
(Rat IL-2 ELISA Kit)
IL-2, ELISA Kit (Cat# AAA9135730)
Full Name
Rat IL-2 ELISA Kit
Gene Names
IL2; IL-2; TCGF; lymphokine
Reactivity
Rat
Pricing
$515/96 Tests | $2,360/5x96 Tests
Activity Data
(Figure. The binding activity of IL13 with IL13Ra1.Interleukin 13 (IL13) is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells. IL13 has effects on immune cells that are similar to those of the closely related cytokine IL4. Although IL13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL13 induces a class of protein-degrading enzymes, known as matrix metalloproteinases (MMPs), in the airways. Furthermore, IL-13 can induce immunoglobulin E (IgE) secretion from activated human B cells. Besides, Interleukin 13 Receptor Alpha 1 (IL13Ra1) has been identified as an interactor of IL13, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat IL13 and recombinant rat IL13Ra1. Briefly, IL13 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL13Ra1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL13 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL13 and IL13Ra1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 13 (IL13), Active Protein (Cat# AAA2105067)
Full Name
Active Interleukin 13 (IL13)
Applications
Cell Culture, Activity Assays
Purity
> 80%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Testing Data #1
(Staining of formalin fixed, paraffin embedded normal human placenta with Rabbit anti Human interleukin-15 )
IL-15, Polyclonal Antibody (Cat# AAA221558)
Full Name
RABBIT ANTI HUMAN INTERLEUKIN-15
Gene Names
IL15; IL-15
Applications
EIA, FN, IP, WB
Pricing
$610/0.1 mg | $2,595/5x0.1 mg
Typical Testing Data/Standard Curve
(Human IL-15 ELISA Kit)
IL-15, ELISA Kit (Cat# AAA9135890)
Full Name
Human IL-15 ELISA Kit
Gene Names
IL15; IL-15
Reactivity
Human
Pricing
$555/96 Tests
