Interleukin-15 Active Protein | mIL 15 active protein

Recombinant Mouse Interleukin-15, His Tag

Cat. Num. AAA143557

• Gene Names: Il15; IL-15; AI503618
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Interleukin-15; Recombinant Mouse Interleukin-15; His Tag; IL 15 Mouse; His; Interleukin-15 Mouse Recombinant; IL-15; MGC9721; Il15; AI503618; mIL 15 His; mIL 15 active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The mouse IL-15 protein solution contains PBS, pH-7.4 and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMNW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILHEYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS
• Sequence Length: 162

• Biological Activity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1 ng/ml, corresponding to a specific activity of 1,000,000IU/mg.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for mIL 15 active protein

Description: Interleukin-15 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (49-162 a.a.) and having a molecular mass of 17.6 kDa. The IL-15 is fused to a 37 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Introduction: The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported.

Product Categories/Family for mIL 15 active protein

Cytokines and Growth Factors; Cytokines; Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 363000984
• NCBI GeneID: 16168
• NCBI Accession #: NP_001241676.1
• NCBI GenBank Nucleotide #: NM_001254747.1
• UniProt Accession #: P48346
• Molecular Weight: 18,593 Da
• NCBI Official Full Name: interleukin-15
• NCBI Official Synonym Full Names: interleukin 15
• NCBI Official Symbol: Il15
• NCBI Official Synonym Symbols: IL-15; AI503618
• NCBI Protein Information: interleukin-15
• UniProt Protein Name: Interleukin-15
• Protein Family: Interleukin
• UniProt Gene Name: Il15
• UniProt Synonym Gene Names: IL-15
• UniProt Entry Name: IL15_MOUSE

Uniprot Description

IL15: Cytokine that stimulates the proliferation of T- lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Cell cycle regulation; Secreted

Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; NK T cell proliferation; positive regulation of immune response; positive regulation of natural killer cell proliferation; negative regulation of smooth muscle cell proliferation; cell maturation; signal transduction; lymph node development; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; extrathymic T cell selection; natural killer cell differentiation; positive regulation of cell proliferation; positive regulation of T cell proliferation; immune response; regulation of T cell differentiation; regulation of defense response to virus by host; hyaluronan metabolic process