Interleukin-4 Active Protein | IL4 active protein

Recombinant Human Interleukin-4 (rHu IL-4)

Cat. Num. AAA546165

• Gene Names: IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Synonyms: Interleukin-4; Recombinant Human Interleukin-4 (rHu IL-4); human IL-4; Human Interleukin-4; IL4 active protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE and HPLC analyses.
• Form/Format: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4; Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
• Sequence Length: 153

• Molecular Weight Information: Approximately 15 kDa, a single non-glycosylated polypeptide chain containing 130 amino acids.
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF1 cells is less than 0.2 ng/ml, corresponding to a Specific Activity of 5 x 106 IU/mg.
• Endotoxin Level: Less than 1EU/mg of rHuIL-4 as determined by LAL method.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.
• Preparation and Storage: This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Related Product Information for IL4 active protein

Interleukin-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.

NCBI and Uniprot Product Information

• NCBI GI #: 4504669
• NCBI GeneID: 3565
• NCBI Accession #: NP_000580.1
• NCBI GenBank Nucleotide #: NM_000589.3
• UniProt Accession #: P05112; Q14630
• Molecular Weight: 17,492 Da
• NCBI Official Full Name: interleukin-4 isoform 1
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: IL4
• NCBI Official Synonym Symbols: BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• NCBI Protein Information: interleukin-4; binetrakin; pitrakinra; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: IL4
• UniProt Synonym Gene Names: IL-4; BSF-1
• UniProt Entry Name: IL4_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; interleukin-4 receptor binding

Biological Process: regulation of isotype switching; negative regulation of osteoclast differentiation; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-dependent; regulation of proton transport; microglial cell activation; female pregnancy; positive regulation of activated T cell proliferation; chemotaxis; response to organic cyclic substance; positive regulation of isotype switching to IgE isotypes; positive regulation of interleukin-10 production; B cell costimulation; connective tissue growth factor biosynthetic process; positive regulation of MHC class II biosynthetic process; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; regulation of immune response; negative regulation of chronic inflammatory response; T-helper 2 type immune response; regulation of phosphorylation; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; defense response to protozoan; T-helper 1 cell lineage commitment; response to ethanol; positive regulation of chemokine biosynthetic process; positive regulation of T cell differentiation; innate immune response in mucosa; positive regulation of tyrosine phosphorylation of Stat5 protein; retina development in camera-type eye; B cell differentiation; response to cytokine stimulus; cellular defense response; positive regulation of transcription factor activity; immune response; positive regulation of transcription from RNA polymerase II promoter; T-helper 2 cell differentiation; positive regulation of defense response to virus by host; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

Disease: Stroke, Ischemic