Rabbit anti-Human Caspase-1 Polyclonal Antibody | anti-CASP1 antibody

Caspase-1 Antibody

Cat. Num. AAA150194

• Gene Names: CASP1; ICE; P45; IL1BC
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Caspase-1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Caspase-1; Polyclonal Antibody; Caspase-1 Antibody; ICE; P45; IL1BC; IL1BCE; Interleukin-1 beta convertase; CASP-1; caspase 1; apoptosis-related cysteine peptidase (interleukin 1; beta; convertase); anti-CASP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Depending on cell lines or tissues used, other cleavage products may be observed.
• Purity/Purification: Caspase-1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 404

• Applicable Applications for anti-CASP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Caspase-1 antibody was raised against a 15 amino acid synthetic peptide from near the middle of human Caspase-1.
• Buffer: Caspase-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Caspase-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Caspase-1 in HeLa cell lysate with Caspase-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of caspase-1 in human spleen tissue with caspase-1 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Caspase-1 in Hela cells with Caspase-1 antibody at 20 μg/mL.)

Related Product Information for anti-CASP1 antibody

Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

NCBI and Uniprot Product Information

• NCBI GI #: 15431328
• NCBI GeneID: 834
• NCBI Accession #: NP_150634
• NCBI GenBank Nucleotide #: NM_033292.3
• UniProt Accession #: P29466; Q53EY6; Q6DMQ1; Q6GSS3; Q6PI75; Q9UCN3; B5MDZ1
• Molecular Weight: 10,417 Da
• NCBI Official Full Name: caspase-1 isoform alpha
• NCBI Official Synonym Full Names: caspase 1, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP1
• NCBI Official Synonym Symbols: ICE; P45; IL1BC
• NCBI Protein Information: caspase-1; IL1B-convertase; CASP1 nirs variant 1; IL-1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• UniProt Protein Name: Caspase-1
• Protein Family: Caspase
• UniProt Gene Name: CASP1
• UniProt Synonym Gene Names: IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
• UniProt Entry Name: CASP1_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Uniprot Description

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.36; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: cytoplasm; extracellular region; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; caspase activator activity

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; membrane hyperpolarization; interleukin-1 beta production; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; mitochondrial depolarization; signal transduction; proteolysis; response to ATP; positive regulation of interleukin-1 alpha secretion; regulation of inflammatory response; response to hypoxia; innate immune response; regulation of autophagy