Rabbit TARDBP Polyclonal Antibody | anti-TARDBP antibody

TARDBP Antibody (N-term)

Cat. Num. AAA9201876

• Gene Names: TARDBP; ALS10; TDP-43
• Reactivity: Human, Mouse, Rat; Predicted: Chicken
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TARDBP; Polyclonal Antibody; TARDBP Antibody (N-term); TAR DNA-binding protein 43; TDP-43; TDP43; anti-TARDBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Chicken
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TARDBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TARDBP.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 1-30
• Sequence Length: 414

• Applicable Applications for anti-TARDBP antibody: Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), ELISA (E/EIA)
• Application Notes: WB~~1:2000; IF~~1:10~50; IHC-P~~1:10~50
• Cellular Location: Nucleus. Cytoplasm. Cytoplasm, Stress granule Note=Continuously travels in and out of the nucleus (PubMed:18957508). Localizes to stress granules in response to oxidative stress (PubMed:19765185).
• Tissue Location: Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

( All lanes : Anti-TARDBP Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with TARDBP Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TARDBP primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TARDBP immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.)

Immunohistochemistry (IHC)

(TARDBP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TARDBP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TARDBP antibody

Background: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle.A ctivation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream ofthe transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided byRefSeq].

Function: RNA-binding protein that is involved invarious steps of RNA biogenesis andprocessing (PubMed:23519609).Preferentially binds, via its two RNArecognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantlylocalized within long introns and in the 3'UTRof mRNAs (PubMed:23519609, PubMed:24240615,PubMed:24464995). In turn,regulates the splicing of many non-codingand protein-coding RNAs including proteinsinvolved in neuronal survival, as well asmRNAs that encode proteins relevant forneurodegenerative diseases (PubMed:21358640, PubMed:29438978). Playsa role in maintaining mitochondrialhomeostasis by regulating the processing ofmitochondrial transcripts (PubMed:28794432). Regulatesalso mRNA stability by recruiting CNOT7/CAF1deadenylase on mRNA 3'UTR leading topoly(A) tail deadenylation and thusshortening (PubMed:30520513). Inresponse to oxidative insult, associates withstalled ribosomes localized to stress granules(SGs) and contributes to cell survival(PubMed:23398327, PubMed:19765185).Participates also in the normal skeletalmuscle formation and regeneration, formingcytoplasmic myo-granules and bindingmRNAs that encode sarcomeric proteins(PubMed:30464263).

Product Categories/Family for anti-TARDBP antibody

Crown Antibodies; Neuroscience

References

Kim, S.H., et al. J. Biol. Chem. 285(44):34097-34105(2010)
Geser, F., et al. Arch. Neurol. 67(10):1238-1250(2010)
Mackenzie, I.R., et al. Lancet Neurol 9(10):995-1007(2010)
Shan, X., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16325-16330(2010)
McKee, A.C., et al. J. Neuropathol. Exp. Neurol. 69(9):918-929(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 6678271
• NCBI GeneID: 23435
• NCBI Accession #: NP_031401.1
• NCBI GenBank Nucleotide #: NM_007375.3
• UniProt Accession #: Q13148; Q53H27; Q6FI92; Q96DJ0; A4GUK4; A4GUK5; A4GUK6; B2R629; B4DJ45; E2PU12
• NCBI Official Full Name: TAR DNA-binding protein 43
• NCBI Official Synonym Full Names: TAR DNA binding protein
• NCBI Official Symbol: TARDBP
• NCBI Official Synonym Symbols: ALS10; TDP-43
• NCBI Protein Information: TAR DNA-binding protein 43
• UniProt Protein Name: TAR DNA-binding protein 43
• Protein Family: TAR DNA-binding protein
• UniProt Gene Name: TARDBP
• UniProt Synonym Gene Names: TDP43; TDP-43
• UniProt Entry Name: TADBP_HUMAN

NCBI Description

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]

Uniprot Description

TARDBP: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. Homodimer. Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent. Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleoplasm; perichromatin fibrils; cytoplasm; nuclear speck; nucleus

Molecular Function: identical protein binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded DNA binding; nucleotide binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; RNA splicing; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; mRNA processing

Disease: Amyotrophic Lateral Sclerosis 10, With Or Without Frontotemporal Dementia