Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with FADS2 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.)
Rabbit FADS2 Polyclonal Antibody | anti-FADS2 antibody
FADS2 Antibody (N-term)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with FADS2 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with FADS2 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.)
Western Blot (WB)
(FADS2 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).)
Western Blot (WB)
(FADS2 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).)
Immunohistochemistry (IHC)
(FADS2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FADS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(FADS2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FADS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(FADS2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(FADS2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
acid desaturase (FADS) gene family. Desaturase enzymes regulate
unsaturation of fatty acids through the introduction of double
bonds between defined carbons of the fatty acyl chain. FADS family
members are considered fusion products composed of an N-terminal
cytochrome b5-like domain and a C-terminal multiple
membrane-spanning desaturase portion, both of which are
characterized by conserved histidine motifs. This gene is clustered
with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is
thought to have arisen evolutionarily from gene duplication based
on its similar exon/intron organization.
Mathias, R.A., et al. J. Lipid Res. 51(9):2766-2774(2010)
Lu, Y., et al. Am. J. Clin. Nutr. 92(1):258-265(2010)
Zabaneh, D., et al. PLoS ONE 5 (8), E11961 (2010) :
Steer, C.D., et al. PLoS ONE 5 (7), E11570 (2010) :
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Uniprot Description
FADS2: Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma- linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum. Belongs to the fatty acid desaturase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; Membrane protein, integral; Lipid Metabolism - unsaturated fatty acid biosynthesis; Lipid Metabolism - alpha-linolenic acid; EC 1.14.19.-; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11q12.2
Cellular Component: endoplasmic reticulum membrane; membrane; integral to plasma membrane
Molecular Function: iron ion binding; heme binding; stearoyl-CoA 9-desaturase activity
Biological Process: unsaturated fatty acid metabolic process; linoleic acid metabolic process; unsaturated fatty acid biosynthetic process
Research Articles on FADS2
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Product Notes
The FADS2 fads2 (Catalog #AAA9210448) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 79-108. The FADS2 Antibody (N-term) reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's FADS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). IF~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the FADS2 fads2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FADS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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