uCHL5 active protein

uCHL5, 1-329aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA204027

• Gene Names: UCHL5; UCH37; CGI-70; INO80R; UCH-L5
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: uCHL5; 1-329aa; Human; His tag; E Coli (Bioactivity Validated); ubiquitin carboxyl-terminal hydrolase isozyme L5; uCH37; uCH-L5; AD-019; CGI-70; INO80R; INO80 complex subunit R; uCHL5 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200mM NaCl, 0.1mM PMSF, 2mM EDTA
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
• Sequence Length: 329

• Applicable Applications for uCHL5 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity: > 500 pmole/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.
• Assay: 1. Prepare a 100ul of recombinant uCH-L5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37Cfor 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.); 2. Add 50ul of 1uM ubiquitin-AMC.; 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.; - ubiquitin-AMC (Enzo, Cat.No, BML-SE211); - 96 Well Polystyrene Microplate, black (greiner bio-one, Cat.No, 655077); - Fluorescent plate reader (PerkinElmer, VICTOR X3)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for uCHL5 active protein

ubiquitin carboxyl-terminal hydrolase isozyme L5 (uCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Recombinant human uCHL5 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for uCHL5 active protein

Ubiquitination

References

Yao T., et al. (2006) Nat. Cell Biol. 8:994-1002; Nishio K, et al. (2009) Biochem Biophys Res Commun. 390 (3):855-60.

NCBI and Uniprot Product Information

• NCBI GI #: 7706753
• NCBI GeneID: 51377
• NCBI Accession #: NP_057068
• NCBI GenBank Nucleotide #: NM_015984.4
• UniProt Accession #: Q9Y5K5; Q5LJA6; Q5LJA7; Q8TBS4; Q96BJ9; Q9H1W5; Q9P0I3; Q9UQN2
• Molecular Weight: 39.7kDa (349aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 1
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L5
• NCBI Official Symbol: UCHL5
• NCBI Official Synonym Symbols: UCH37; CGI-70; INO80R; UCH-L5
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L5
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L5
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: UCHL5
• UniProt Synonym Gene Names: UCH37; UCH-L5

Uniprot Description

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.