Latent TGF-beta 1 Active Protein | TGFB active protein

Human Latent TGF-beta 1 Protein, His Tag

Cat. Num. AAA9718692

• Gene Names: TGFB1; CED; LAP; DPD1; TGFB; IBDIMDE; TGFbeta
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Latent TGF-beta 1; Human Latent TGF-beta 1 Protein; His Tag; Recombinant Human Latent TGF-beta 1 Protein; His Tag (Transforming growth factor beta-1) expressed in Human Cells; CED; DPD1; LAP; TGF-beta 1; TGFbeta; Transforming growth factor beta-1; TGFB1; TGFB active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence: Amino acid sequence derived from full length of human TGFbeta1 (NP_000651.3) (Met 1-Ser 390) was fused with a C-terminal polyhistidine tag.
• Sequence Length: 390

• Species: Human
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method
• Protein Length: The recombinant human latent TGFbeta1 consists of 370 amino acids and has a calculated molecular mass of 42.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TGFbeta1 is approximately 16, 38 and 55 kDa corresponding to mature TGFbeta1, LAP protein and inacitve latent TGFbeta1 respectively due to glycosylation. In non-reduced SDS-PAGE, it migrates as an approximately 110 kDa protein consisting of a TGFbeta1 homodimer non-covalently linked to a LAP homodimer.
• Activity: Measured by its ability to bind biotinylated Human ALK1-Fc in functional Elisa.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Latent TGF-beta 1 protein, His Tag in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Ships on blue ice. Lyophilized Human Latent TGF-beta 1 protein, His Tag product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human Latent TGF-beta 1 protein, His Tag.)

Related Product Information for TGFB active protein

TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-gamma, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.

NCBI and Uniprot Product Information

• NCBI GI #: 63025222
• NCBI GeneID: 7040
• NCBI Accession #: NP_000651.3
• NCBI GenBank Nucleotide #: NP_000651.3
• UniProt Accession #: P01137
• NCBI Official Full Name: transforming growth factor beta-1 proprotein preproprotein
• NCBI Official Synonym Full Names: transforming growth factor beta 1
• NCBI Official Symbol: TGFB1
• NCBI Official Synonym Symbols: CED; LAP; DPD1; TGFB; IBDIMDE; TGFbeta
• NCBI Protein Information: transforming growth factor beta-1 proprotein; transforming growth factor beta-1

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016]