Cathepsin X/Z/P Active Protein | Ctsz active protein

Recombinant Cathepsin X/Z/P

Cat. Num. AAA201142

• Gene Names: Ctsz; CTSX; AI787083; AU019819; D2Wsu143e
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: Cathepsin X/Z/P; Recombinant Cathepsin X/Z/P; CTSZ; AI787083; AU019819; CTSX; D2Wsu143e; Ctsz active protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 23-306aa
• Sequence: ARARLYFRSG QTCYHPIRGD QLALLGRRTY PRPHEYLSPA DLPKNWDWRN VNGVNYASVT RNQHIPQYCG SCWAHGSTSA MADRINIKRK GAWPSILLSV QNVIDCGNAG SCEGGNDLPV WEYAHKHGIP DETCNNYQAK DQDCDKFNQC GTCTEFKECH TIQNYTLWRV GDYGSLSGRE KMMAEIYANG PISCGIMATE MMSNYTGGIY AEHQDQAVIN HIISVAGWGV SNDGIEYWIV RNSWGEPWGE KGWMRIVTST YKGGTGDSYN LAIESACTFG DPIV LEHHHH HH
• Sequence Length: 306

• Applicable Applications for Ctsz active protein: SDS-PAGE, Enzyme Activity
• Species: Mouse
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Ala 23
• Biological Activity: Specific activity is > 3,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-PLGL-Dpa-AR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for Ctsz active protein

Cathepsin X/Z/P, also known as cathepsin Z/X, is a relatively new cysteine protease of the papain family. Compared to other members of the papain family, It has a short proregion and unique insertions. Acting as a carboxypeptidase, It displays a unique specificity. Recombinant mouse Cathepsin X/Z/P, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Ctsz active protein

Cancer

References

Santamaria., et al. (1998) J. Biol. Chem. 273:16816-16823. Menard., et al. (2001) Biol. Chem. 382:839-845.

NCBI and Uniprot Product Information

• NCBI GI #: 11968166
• NCBI GeneID: 64138
• NCBI Accession #: NP_071720.1
• NCBI GenBank Nucleotide #: NP_071720.1
• UniProt Accession #: Q9WUU7
• Molecular Weight: 32.8kDa (292aa); 28-40kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: cathepsin Z preproprotein
• NCBI Official Synonym Full Names: cathepsin Z
• NCBI Official Symbol: Ctsz
• NCBI Official Synonym Symbols: CTSX; AI787083; AU019819; D2Wsu143e
• NCBI Protein Information: cathepsin Z
• UniProt Protein Name: Cathepsin Z
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsz
• UniProt Entry Name: CATZ_MOUSE

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate a mature enzyme with carboxypeptidase activity. An enzymatically inactive form of the protein, that is associated with the propeptide, may be involved in cancer cell invasion and proliferation. Homozygous knockout mice for this gene exhibit impaired cancer cell invasion in a breast cancer model. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSZ: Exhibits carboxy-monopeptidase as well as carboxy- dipeptidase activity. Belongs to the peptidase C1 family.

Protein type: EC 3.4.18.1; Protease

Cellular Component: endoplasmic reticulum; extracellular space; intracellular membrane-bound organelle; lysosome

Molecular Function: cysteine-type endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process