ACP5 active protein

ACP5, 22-325aa, Human, His tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206334

• Gene Names: ACP5; HPAP; TRAP; TRACP5a; TRACP5b; TrATPase
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ACP5; 22-325aa; Human; His tag; Baculovirus (Bioactivity Validated); Tartrate-resistant acid phosphatase type 5; SPENCDI; TRAP; ACP5 active protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNH DHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVL VAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGG FAYVEISSKE MTVTYIEASG KSLFKTRLPR RARPHHHHHH
• Sequence Length: 325

• Applicable Applications for ACP5 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 5,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ACP5 active protein

ACP5, also known as tartrate-resistant acid phosphatase type 5, is involved in osteopontin and bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Recombinant human ACP5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for ACP5 active protein

Immunology

References

Janckila A.J. et al., (1992) Clin. Biochem. 25:437-443.; Hayman A.R. et al., (1991) Biochem. J. 277:631-634.

NCBI and Uniprot Product Information

• NCBI GI #: 161377453
• NCBI GeneID: 54
• NCBI Accession #: NP_001104505
• NCBI GenBank Nucleotide #: NM_001111035.2
• UniProt Accession #: P13686; Q2TAB1; Q6IAS6; Q9UCJ5; Q9UCJ6; Q9UCJ7; A8K3V2
• Molecular Weight: 35.1kDa (310aa) 28-40kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: tartrate-resistant acid phosphatase type 5
• NCBI Official Synonym Full Names: acid phosphatase 5, tartrate resistant
• NCBI Official Symbol: ACP5
• NCBI Official Synonym Symbols: HPAP; TRAP; TRACP5a; TRACP5b; TrATPase
• NCBI Protein Information: tartrate-resistant acid phosphatase type 5
• UniProt Protein Name: Tartrate-resistant acid phosphatase type 5
• Protein Family: Acyl carrier protein
• UniProt Gene Name: ACP5
• UniProt Synonym Gene Names: TR-AP; TrATPase

NCBI Description

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

Uniprot Description

Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.