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Immunofluorescent (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized mouse skin tissue labeling Pdx1 with MBS9204466 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasm staining on mouse skin tissue. The nuclear counter stain is DAPI (blue).)

Rabbit anti-Mouse Srms Polyclonal Antibody | anti-Srms antibody

Mouse Srms Antibody (Center)

Gene Names
Srms; srm; A230069J08Rik
Reactivity
Mouse
Applications
Immunofluorescence, Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Srms; Polyclonal Antibody; Mouse Srms Antibody (Center); Tyrosine-protein kinase Srms; PTK70; Srm; anti-Srms antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Srms antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 113-147 amino acids from the Central region of Mouse Srms.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
496
Applicable Applications for anti-Srms antibody
Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes
IF~~1:25
WB~~1:1000
Clone Names
RB50486
Function
Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues. May be involved in proliferation or differentiation of keratinocytes in the skin.
Function
Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues. Also phosphorylates KHDRBS1/SAM68 and VIM on tyrosine residues. Phosphorylation of KHDRBS1 is EGF-dependent.
Cellular Location
Cytoplasm {ECO:0000250|UniProtKB:Q9H3Y6}. Note=Localizes to punctate cytoplasmic structures {ECO:0000250|UniProtKB:Q9H3Y6}
Tissue Location
Higher expression in liver, lung, thymus and skin than in brain, kidney, heart and spleen (PubMed:9226137). In skin, highly expressed in keratinocytes (PubMed:9226137). Abundant in lung, liver, spleen, kidney and testis and is also detected in the cerebrum (PubMed:7935409).
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunofluorescent

(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized mouse skin tissue labeling Pdx1 with MBS9204466 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasm staining on mouse skin tissue. The nuclear counter stain is DAPI (blue).)

Immunofluorescent (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized mouse skin tissue labeling Pdx1 with MBS9204466 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasm staining on mouse skin tissue. The nuclear counter stain is DAPI (blue).)

Western Blot (WB)

(Anti-Srms Antibody (C-term) at 1:2000 dilution + mouse liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-Srms Antibody (C-term) at 1:2000 dilution + mouse liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western blot analysis of lysates from mouse liver, mouse spleen tissue lysate (from left to right), using Srms Antibody (Center). MBS9204466 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from mouse liver, mouse spleen tissue lysate (from left to right), using Srms Antibody (Center). MBS9204466 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-Srms antibody
Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues. May be involved in proliferation or differentiation of keratinocytes in the skin.
Product Categories/Family for anti-Srms antibody
References
Kawachi Y.,et al.Exp. Dermatol. 6:140-146(1997).
Kohmura N.,et al.Mol. Cell. Biol. 14:6915-6925(1994).
Wall M.,et al.Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
55758
NCBI Official Full Name
Tyrosine-protein kinase Srms
NCBI Official Synonym Full Names
src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites
NCBI Official Symbol
Srms
NCBI Official Synonym Symbols
srm; A230069J08Rik
NCBI Protein Information
tyrosine-protein kinase Srms
UniProt Protein Name
Tyrosine-protein kinase Srms
Protein Family
UniProt Gene Name
Srms
UniProt Synonym Gene Names
Srm
UniProt Entry Name
SRMS_MOUSE

Uniprot Description

SRMS: a tyrosine kinase of the Src family.

Protein type: Protein kinase, tyrosine (non-receptor); Motility/polarity/chemotaxis; Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; Src family

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm

Molecular Function: transferase activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; ATP binding; protein kinase activity; receptor binding

Biological Process: cell migration; innate immune response; actin cytoskeleton organization and biogenesis; cell differentiation; protein amino acid phosphorylation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation

Research Articles on Srms

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Product Notes

The Srms srms (Catalog #AAA9204466) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Srms Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Srms can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). IF~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the Srms srms for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Srms, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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