Rabbit anti-Human RAD52 Polyclonal Antibody | anti-RAD52 antibody

Rabbit Polyclonal to Human RAD52

Cat. Num. AAA240523

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: RAD52; Polyclonal Antibody; Rabbit Polyclonal to Human RAD52; Anti-RAD52 Antibody (aa360-375) IHC-plus; RAD52 (S. cerevisiae) homolog; RAD52 homolog (S. cerevisiae); Recombination protein RAD52; Human RAD52; anti-RAD52 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: aa 360 - 375 of the Human Rad 52 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 418

• Applicable Applications for anti-RAD52 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000 - 1:20000), IHC-P (2.5 ug/ml), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human RAD52.
• Immunogen Type: Synthetic peptide
• Immunogen: RAD52 antibody was raised against synthetic peptide from human RAD52.
• Antigen Modification: aa360-375
• Preparation and Storage: Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.

Immunohistochemistry (IHC)

(Anti-RAD52 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-Human RAD52 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B). ~30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~63.0 kD is detected. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] (diluted 1:5000 for 45 min. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by.)

NCBI and Uniprot Product Information

• NCBI GI #: 109637798
• NCBI GeneID: 5893
• NCBI Accession #: NP_602296.2
• NCBI GenBank Nucleotide #: NM_134424.3
• UniProt Accession #: P43351; Q13205; Q9Y5T7; Q9Y5T8; Q9Y5T9
• Molecular Weight: 12,943 Da
• NCBI Official Full Name: DNA repair protein RAD52 homolog isoform a
• NCBI Official Synonym Full Names: RAD52 homolog (S. cerevisiae)
• NCBI Official Symbol: RAD52
• NCBI Protein Information: DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23
• UniProt Protein Name: DNA repair protein RAD52 homolog
• Protein Family: RAD52 motif-containing protein
• UniProt Gene Name: RAD52
• UniProt Entry Name: RAD52_HUMAN

NCBI Description

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Uniprot Description

RAD52: a protein involved in DNA double strand breaks and recombination. Associates with and is phosphorylated by c-Abl. Belongs to the RAD52/RAD22 family.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 12p13-p12.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding

Biological Process: DNA recombinase assembly; double-strand break repair; DNA repair; double-strand break repair via homologous recombination; DNA recombination