Typical Testing Data/Standard Curve (for reference only)
Mouse Chemokine (C-X-C Motif) Ligand 2 (CXCL2) ELISA Kit | CXCL2 elisa kit
Mouse Chemokine (C-X-C Motif) Ligand 2 (CXCL2)(Sandwich ELISA) ELISA Kit
Gene Names
Cxcl2; GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a
Reactivity
Mouse
Applications
ELISA
Synonyms
Chemokine (C-X-C Motif) Ligand 2 (CXCL2); Mouse Chemokine (C-X-C Motif) Ligand 2 (CXCL2)(Sandwich ELISA) ELISA Kit; Mini Samples ELISA Kit for Chemokine (C-X-C Motif) Ligand 2 (CXCL2); CXCL2/Chemokine (C-X-C Motif) Ligand 2; GROb; SCYB2; MIP2; GRO2; MIP2a; MGSAb; CINC2a; HSF; Macrophage inflammatory protein 2-alpha; Hematopoietic synergistic factor; Growth Regulated Oncogene Beta; CXCL2 elisa kit
Reactivity
Mouse
Specificity
Reactive with Mouse Chemokine (C-X-C Motif) Ligand 2 (CXCL2)
This assay has high sensitivity and excellent specificity for detection of Mini Samples Chemokine (C-X-C Motif) Ligand 2 (CXCL2). No significant cross-reactivity or interference between Mini Samples Chemokine (C-X-C Motif) Ligand 2 (CXCL2) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Mini Samples Chemokine (C-X-C Motif) Ligand 2 (CXCL2). No significant cross-reactivity or interference between Mini Samples Chemokine (C-X-C Motif) Ligand 2 (CXCL2) and analogues was observed.
Applicable Applications for CXCL2 elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Type
Sandwich ELISA-Mini Samples
Sample Types
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
5.5pg/mL
Detection Method
Double-antibody Sandwich
Assay Length
3 hours
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Chemokine (C-X-C Motif) Ligand 2 (CXCL2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Chemokine (C-X-C Motif) Ligand 2 (CXCL2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Chemokine (C-X-C Motif) Ligand 2 (CXCL2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 6 (IGFBP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 6 (IGFBP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 6 (IGFBP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Like Growth Factor Binding Protein 6 (IGFBP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 25ul standard or sample to each well. Incubate 1 hour at 37 degree C
3. Aspirate and add 25ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 25ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 25ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 20ul Stop Solution. Read at 450nm immediately.
2. Add 25ul standard or sample to each well. Incubate 1 hour at 37 degree C
3. Aspirate and add 25ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 25ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 25ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 20ul Stop Solution. Read at 450nm immediately.
Preparation and Storage
Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for CXCL2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,621 Da
NCBI Official Full Name
C-X-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 2
NCBI Official Symbol
Cxcl2
NCBI Official Synonym Symbols
GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a
NCBI Protein Information
C-X-C motif chemokine 2; macrophage inflammatory protein 2; small inducible cytokine subfamily, member 2
UniProt Protein Name
C-X-C motif chemokine 2
Protein Family
UniProt Gene Name
Cxcl2
UniProt Synonym Gene Names
Mip-2; Mip2; Scyb2; MIP2
UniProt Entry Name
CXCL2_MOUSE
Uniprot Description
Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.
Research Articles on CXCL2
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Product Notes
The Mouse CXCL2 cxcl2 (Catalog #AAA2125247) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2125247 ELISA Kit recognizes Mouse CXCL2. AAA Biotech's Chemokine (C-X-C Motif) Ligand 2 (CXCL2) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the CXCL2 cxcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chemokine (C-X-C Motif) Ligand 2 (CXCL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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