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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using KIF22 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)

Rabbit KIF22 Polyclonal Antibody | anti-KIF22 antibody

KIF22 Polyclonal Antibody

Gene Names
KIF22; KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
KIF22; Polyclonal Antibody; KIF22 Polyclonal Antibody; A-328A3.2; KID; KNSL4; OBP; OBP-1; OBP-2; SEMDJL2; anti-KIF22 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
QGAPLLSTPKRERMVLMKTVEEKDLEIERLKTKQKELEAKMLAQKAEEKENHCPTMLRPLSHRTVTGAKPLKKAVVMPLQLIQEQAASPNAEIHILKNKGRKRKLESLDALEPEEKAEDCWELQISPELLAHGRQKILDLLNEGSARDLRSLQRIGPKKAQLIVGWRELHGPFSQVEDLERVEGITGKQMESFLKANILGLAAGQRCGAS
Sequence Length
597
Applicable Applications for anti-KIF22 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 456-665 of human KIF22 (NP_015556.1).
Immunogen Species
Human
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using KIF22 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using KIF22 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon using KIF22 antibody (MBS9135271) at dilution of 1:100 (40xlens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon using KIF22 antibody (MBS9135271) at dilution of 1:100 (40xlens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using KIF22 antibody (MBS9135271) at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon carcinoma using KIF22 antibody (MBS9135271) at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using KIF22 antibody (MBS9135271) at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using KIF22 antibody (MBS9135271) at dilution of 1:100 (40x lens).)
Related Product Information for anti-KIF22 antibody
The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-KIF22 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 66kDa; 73kDa
Observed: 73kDa
NCBI Official Full Name
kinesin-like protein KIF22 isoform 2
NCBI Official Synonym Full Names
kinesin family member 22
NCBI Official Symbol
KIF22
NCBI Official Synonym Symbols
KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2
NCBI Protein Information
kinesin-like protein KIF22
UniProt Protein Name
Kinesin-like protein KIF22
Protein Family
UniProt Gene Name
KIF22
UniProt Synonym Gene Names
KID; KNSL4

NCBI Description

The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (). Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells (PubMed:25743205).

Research Articles on KIF22

Similar Products

Product Notes

The KIF22 kif22 (Catalog #AAA9135271) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's KIF22 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:200 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the KIF22 kif22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QGAPLLSTPK RERMVLMKTV EEKDLEIERL KTKQKELEAK MLAQKAEEKE NHCPTMLRPL SHRTVTGAKP LKKAVVMPLQ LIQEQAASPN AEIHILKNKG RKRKLESLDA LEPEEKAEDC WELQISPELL AHGRQKILDL LNEGSARDLR SLQRIGPKKA QLIVGWRELH GPFSQVEDLE RVEGITGKQM ESFLKANILG LAAGQRCGAS. It is sometimes possible for the material contained within the vial of "KIF22, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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