Western Blot (WB)
(Western blot analysis of lysate from MAD-MB-231 cell line, using USP17 Antibody (C-term). MBS9211987 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Rabbit anti-Human USP17 Polyclonal Antibody | anti-USP17L24 antibody
USP17 Antibody (C-term)
Reactivity
Human
Applications
ELISA, Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
USP17; Polyclonal Antibody; USP17 Antibody (C-term); Ubiquitin carboxyl-terminal hydrolase 17-like protein 24; Deubiquitinating enzyme 17; Ubiquitin thioesterase 17; Ubiquitin-specific-processing protease 17; USP17L24; anti-USP17L24 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This USP17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-524 amino acids from the C-terminal region of human USP17.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
496-524
Sequence Length
530
Applicable Applications for anti-USP17L24 antibody
WB,FC,ELISA
Application Notes
WB~~1:1000
FC~~1:10~50
FC~~1:10~50
Function
Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes that may include cell proliferation, progression through the cell cycle, apoptosis, cell migration, and the cellular response to viral infection.
Cellular Location
Nucleus, nucleolus. Endoplasmic reticulum
Tissue Location
Expressed in heart, brain, liver and skeletal
muscle
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysate from MAD-MB-231 cell line, using USP17 Antibody (C-term). MBS9211987 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of lysate from MAD-MB-231 cell line, using USP17 Antibody (C-term). MBS9211987 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(USP17 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the USP17 antibody detected the USP17 protein (arrow).)
Western Blot (WB)
(USP17 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the USP17 antibody detected the USP17 protein (arrow).)
Flow Cytometry (FC/FACS)
(USP17 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(USP17 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-USP17L24 antibody
USP17 recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins (By similarity).
Product Categories/Family for anti-USP17L24 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59711
NCBI Official Full Name
ubiquitin specific peptidase 17-like family member 25
NCBI Official Synonym Full Names
ubiquitin specific peptidase 17-like family member 25
NCBI Official Symbol
USP17L25
NCBI Protein Information
ubiquitin specific peptidase 17-like family member 25
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 17-like protein 24
Protein Family
UniProt Gene Name
USP17L24
UniProt Synonym Gene Names
USP17; USP17H; USP17I; USP17J; USP17K; USP17L; USP17M
UniProt Entry Name
U17LO_HUMAN
Uniprot Description
USP17: Functions in cell apoptosis. Cleaves ubiquitin fusion protein substrates. May also bind hyaluronan and RNA. Belongs to the peptidase C19 family. USP17 subfamily.
Protein type: EC 3.4.19.12; Protease; Ubiquitin-specific protease; Nucleolus
Chromosomal Location of Human Ortholog: 4p15
Cellular Component: endoplasmic reticulum; nucleolus
Molecular Function: cysteine-type peptidase activity
Biological Process: ubiquitin-dependent protein catabolic process; apoptosis
Similar Products
Product Notes
The USP17L24 usp17l24 (Catalog #AAA9211987) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 496-524. The USP17 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP17 can be used in a range of immunoassay formats including, but not limited to, WB,FC,ELISA. WB~~1:1000 FC~~1:10~50. Researchers should empirically determine the suitability of the USP17L24 usp17l24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP17, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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