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Reactivity
Gene Name
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Application
Clone
3255 results for "Primary Antibodies Detection antibody" - showing 900-950
Western Blot (WB)
((0. 01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
TNNT3, Polyclonal Antibody (Cat# AAA423125)
Full Name
Goat anti-TNNT3 Antibody
Gene Names
TNNT3; TNTF
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
((2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
ICAM1, Polyclonal Antibody (Cat# AAA422939)
Full Name
Goat anti-ICAM1 Antibody
Gene Names
ICAM1; BB2; CD54; P3.58
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
((0.3ug/ml) staining of A549 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
cytochrome P450 1A1, Polyclonal Antibody (Cat# AAA423384)
Full Name
Goat anti-cytochrome P450 1A1 Antibody
Gene Names
CYP1A1; AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 by Western Blot. Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 2ug/ml of Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody followed by HRPconjugated Mouse IgG Secondary Antibody. A specific band was detected for Cytosolic Sulfotransferase 4A1/SULT4A1 at approximately 35kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
SULT4A1, Monoclonal Antibody (Cat# AAA647252)
Full Name
SULT4A1 (sulfotransferase family 4A, Member 1, Brain sulfotransferase-like protein, Nervous system sulfotransferase, brain sulphotransferase-like, nervous system cytosolic sulfotransferase, sulfotransferase-related protein)
Gene Names
SULT4A1; NST; BRSTL1; SULTX3; BR-STL-1; DJ388M5.3; hBR-STL-1
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purified
Purified by Protein A/G affinity chromatography.
Purified by Protein A/G affinity chromatography.
Pricing
$745/0.1 mg | $3,355/5x0.1 mg
Western Blot (WB)
(Detection of Human MERIT40 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gel was loaded with 20ug of cytoplasmic (Cyto) and 10 μg of nuclear extracts (Nuc). PVDF Membrane was probed with 1ug/ml of Human MERIT40 Antigen Affinitypurified Polyclonal Antibody followed by HRPconjugated Sheep IgG Secondary Antibody. A specific band was detected for MERIT40 at approximately 40kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
HSPC142, Polyclonal Antibody (Cat# AAA646739)
Full Name
HSPC142 (BRCA1-A Complex Subunit MERIT40, Chromosome 19 Open Reading Frame 62, C19orf62, FLJ20571, Mediator of RAP80 Interactions and Targeting Subunit of 40kD, MERIT40, New Component of the BRCA1-A Complex, NBA1)
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$805/0.1 mg | $3,615/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-AKT Polyclonal Antibody . Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-AKT Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.)
AKT, Polyclonal Antibody (Cat# AAA8001644)
Full Name
AKT Antibody: Biotin
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Testing Data
(ELISA test of chicken anti- COVID-19 spike S1 protein.Antibodies: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike (S1) antibody (at dilutions from 1 in 500 to 1 in 64,000). A direct ELISA was performed using purified COVID-19 Spike protein (S1) as coating antigen and the anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Spike (S1) antibody as the primary antibody. Secondary: Rabbit anti-chicken IgY Fc HRP conjugate at 65ng/ml. Preimmune sera gave signals of approximately 0.110 or less at all comparable dilutions.)
COVID 19 Spike S1 Coronavirus, Polyclonal Antibody (Cat# AAA378286)
Full Name
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike S1 Domain Antibody
Reactivity
Virus
Applications
EIA
Pricing
$220/0.05 mL | $280/0.1 mL | $470/0.2 mL | $1,885/5x0.2 mL
Western Blot (WB)
((0.1ug/ml) staining of Human (A) and Mouse (B) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
cardiac troponin T, Polyclonal Antibody (Cat# AAA423633)
Full Name
Goat anti-cardiac troponin T (aa201-213) Antibody
Gene Names
TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
Reactivity
Tested: Human, Mouse, Pig; Expected from sequence similarity: Human, Mouse, Rat, Cat, Dog, Pig, Cow
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western Blot of MBS617987 performed from 50ug of transfected 293 T cells. Primary antibody MBS617987(1:500 dilution) was incubated O/N at 4°C. Secondary rabbit HRP antibody was incubated 1 hour at RT followed by ECL detection.)
Trick2, Polyclonal Antibody (Cat# AAA617987)
Full Name
Trick2 (TRAIL Receptor Inducer of Cell Killing 2)
Gene Names
TNFRSF10B; DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$495/0.1 mg | $2,220/5x0.1 mg
Western Blot (WB)
(Western blot using Immunochemicals' affinity purified anti- p90 RSK1 pS732 antibody shows detection of a band ~90kD in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL)
RSK1, Polyclonal Antibody (Cat# AAA619189)
Full Name
RSK1, phosphorylated (Ser732) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Prote
Gene Names
RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$855/0.1 mg | $3,840/5x0.1 mg
DyLight488 Conjugated Mouse IgM, Detection Kit (Cat# AAA177108)
Full Name
DyLight488 Conjugated Mouse IgM SABC Kit
Applications
IHC, IHC, ICC
Pricing
$470/1 Kit | $2,110/5x1 Kit
Testing Data
(Figure A. PE conjugated Mouse anti Human CD11c and Amethyst Orange conjugated Mouse IgG2a isotype control . Figure B. PE conjugated Mouse anti Human CD11c and Amethyst Orange conjugated Mouse anti Human HLA DP DQ DR . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
HLA DP DQ DR, Monoclonal Antibody (Cat# AAA225865)
Full Name
Mouse Anti Human HLA DP DQ DR: Amethyst Orange
Reactivity
Human
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
$500/0.1 mg | $2,090/5x0.1 mg
Dot Blot (DB)
(Dot blot analysis of anti-CDK11-S39 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.)
CDK1, Polyclonal Antibody (Cat# AAA624513)
Full Name
CDK1, phosphorylated (Ser39) (Cell Division Protein Kinase, CDK1, Cyclin-dependent Kinase 1, Cell Division Control Protein 2 Homolog, p34 Protein Kinase)
Gene Names
CDK1; CDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222
Reactivity
Human
Applications
EL/EIA, WB, IHC, GS/EMSA
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~21.6 kDa KRAS protein using Rabbit Anti-KRAS Polyclonal Antibody (SPC-777). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KRAS Polyclonal Antibody (SPC-777) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.6 kDa. Other Band(s): ~29 kDa (PTM).)
KRAS, Polyclonal Antibody (Cat# AAA8001419)
Full Name
KRAS Antibody: ATTO 488
Gene Names
KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone MGL3R. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
Ubiquitin, Monoclonal Antibody (Cat# AAA809233)
Full Name
Ubiquitin Antibody, Clone MGL3R: Biotin
Gene Names
UBB; HEL-S-50
Reactivity
Species Independent
Applications
WB, EIA
Purity
Protein A Purified
Pricing
$520/0.1 mg | $2,125/5x0.1 mg
Immunohistochemistry (IHC)
(High grade squamous intraepithelial lesion of the uterine cervix stained with mouse anti-p16 (clone R15-A) monoclonal antibody, shows significant nuclear and cytoplasmic positivity of target cells. Formalin fixed, paraffin embedded human tissues (4um sections) stained according to related datasheet.)
p16, Monoclonal Antibody (Cat# AAA684300)
Full Name
Anti-p16, Mouse monoclonal antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
IHC-P
Pricing
$375/7 mL (RTU) | $355/0.1 mL (Concentrate) | $580/0.5 mL (Concentrate) | $875/1 mL (Concentrate) | $465/0.2 mL (Concentrate) | $540/15 mL (RTU) | $195/1 mL (RTU) | $205/0.04 mL (Concentrate) | $3,675/5x1 mL (Concentrate)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Adenosine receptor A1 Antibody (D) Composite.)
Adenosine receptor A1, Polyclonal Antibody (Cat# AAA8000231)
Full Name
Adenosine receptor A1 Antibody: ATTO 488
Gene Names
ADORA1; RDC7
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot using Immunochemicals Affinity Purified Anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 _g of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (p/n B501-0500) and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reacting with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRP- conjugated a-Rabbit IgG (p/n 611-103-122) for 1 hour at room temperature. Detection was by ECL.)
Smad1, Polyclonal Antibody (Cat# AAA621285)
Full Name
Smad1, phosphorylated (Ser206) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$855/0.1 mg | $3,840/5x0.1 mg
Western Blot (WB)
((1ug/ml) staining HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
KAP1, Polyclonal Antibody (Cat# AAA423261)
Full Name
Goat anti-KAP1 Antibody
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
((0.5ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Nanog, Polyclonal Antibody (Cat# AAA423265)
Full Name
Goat anti-Nanog (mouse aa270-284) Antibody
Gene Names
Nanog; ENK; ecat4; 2410002E02Rik
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
((1ug/ml) staining of Human lung (A) and Human Umbical cord (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour.)
ECSM2, Polyclonal Antibody (Cat# AAA423246)
Full Name
Goat anti-ECSM2 Antibody
Gene Names
ECSCR; ARIA; ECSM2
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)
CBP, Polyclonal Antibody (Cat# AAA8000962)
Full Name
CBP Antibody: HRP
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with UVRAG (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1850b UVRAG (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). UVRAG immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
UVRAG, Polyclonal Antibody (Cat# AAA6001910)
Full Name
UVRAG, CT (UV Radiation Resistance-associated Gene Protein, p63, DHTX, VPS38)
Gene Names
UVRAG; p63; DHTX; VPS38
Reactivity
Human, Mouse
Applications
EL/EIA, WB, IP, IHC
Purity
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
TRB3 / TRIB3, Polyclonal Antibody (Cat# AAA2400246)
Full Name
Anti-TRB3 / TRIB3 Antibody (Internal) IHC-plus
Gene Names
TRIB3; NIPK; SINK; TRB3; SKIP3; C20orf97
Reactivity
Mouse, Rat, Human
Applications
IHC, IF, WB, EIA
Purity
Peptide Column Affinity Chromatography
Pricing
$585/0.05 mg | $2,585/5x0.05 mg
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 22.1 kDa RAB1B protein using Rabbit Anti-RAB1B Polyclonal Antibody (SPC-684). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAB1B Polyclonal Antibody (SPC-684) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 22.1 kDa.)
RAB1B, Polyclonal Antibody (Cat# AAA8000065)
Full Name
RAB1B Antibody: RPE
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~90 kDa HSP90 alpha/beta protein using Llama Anti-HSP90 alpha/beta Polyclonal Antibody (SPC-745). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Llama Anti-HSP90 alpha/beta Polyclonal Antibody (SPC-745) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Llama HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~90 kDa.)
HSP90 alpha/beta, Polyclonal Antibody (Cat# AAA8000857)
Full Name
HSP90 alpha/beta Antibody: RPE
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human; Mouse; Rat
Applications
WB, EIA
Purity
Peptide Affinity Purified
Pricing
$495/0.1 mg | $1,995/5x0.1 mg
Western Blot (WB)
(Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and DYKDDDDK-sHIPK3 (aa 159 to 1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-DYKDDDDK antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and DYKDDDDK-sHIPK3.)
HIPK3, Polyclonal Antibody (Cat# AAA641944)
Full Name
HIPK3, CT (HIPK3, DYRK6, FIST3, PKY, Homeodomain-interacting protein kinase 3, Androgen receptor-interacting nuclear protein kinase, Fas-interacting serine/threonine-protein kinase, Homolog of protein kinase YAK1)
Gene Names
HIPK3; PKY; YAK1; DYRK6; FIST3
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Western Blot (WB)
((2ug/ml) staining of Kidney (A), Testis (B) and Uterus (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
TIM-1, Polyclonal Antibody (Cat# AAA423514)
Full Name
Goat anti-TIM-1 Antibody
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Flow Cytometry
(Flow Cytometric analysis using Mouse anti-rat IgM antibodyStaining of rat splenocytes with Mouse IgG1 K Isotype Control (FITC) (open histogram) or Anti-Rat IgM (FITC) (filled histogram). Total viable cells were used for analysis.)
Mouse anti-rat IgM antibody (PE), Monoclonal Antibody (Cat# AAA835033)
Full Name
Mouse anti-rat IgM antibody (PE)
Applications
FC/FACS
Pricing
$490/0.1 mg | $2,170/5x0.1 mg
Western Blot (WB)
((1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Nalp12/Nlrp12, Polyclonal Antibody (Cat# AAA423301)
Full Name
Goat anti-Nalp12/Nlrp12 (rodent) Antibody
Gene Names
Nlrp12; Nalp12; PYPAF7
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)
CBP, Polyclonal Antibody (Cat# AAA8000951)
Full Name
CBP Antibody: ATTO 488
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.8 kDa c-Myc protein using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.8 kDa.)
c-Myc, Polyclonal Antibody (Cat# AAA8000493)
Full Name
c-Myc Antibody: FITC
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
((1ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
JUND, Polyclonal Antibody (Cat# AAA423387)
Full Name
Goat anti-JUND Antibody
Gene Names
JUND; AP-1
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Dot Blot (DB)
(Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2A8.1. Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.)
Crotonaldehyde, Monoclonal Antibody (Cat# AAA809001)
Full Name
Crotonaldehyde Antibody, Clone 2A8.1: HRP
Reactivity
ALL
Applications
WB, EIA
Purity
Protein G Purified
Pricing
$545/0.1 mg | $2,215/5x0.1 mg
Western Blot (WB)
((0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
PTCD2, Polyclonal Antibody (Cat# AAA422970)
Full Name
Goat anti-PTCD2 (aa214-226) Antibody
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
((2ug/ml) staining of HeLa (A), HepG2 (B) and U2OS (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
SCRIB/Scribble, Antibody (Cat# AAA423847)
Full Name
Goat Anti-SCRIB/Scribble Antibody
Gene Names
SCRIB; CRIB1; SCRB1; SCRIB1; Vartul
Reactivity
Tested: Human
Expected from sequence similarity: Human
Expected from sequence similarity: Human
Applications
EIA, IF
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
DyLight488 Conjugated Human IgG, Detection Kit (Cat# AAA177107)
Full Name
DyLight488 Conjugated Human IgG SABC Kit
Applications
IHC, IHC, ICC
Pricing
$440/1 Kit | $1,965/5x1 Kit
Western Blot (WB)
((1ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Stap2, Polyclonal Antibody (Cat# AAA423298)
Full Name
Goat anti-Stap2 (mouse) Antibody
Gene Names
Stap2; STAP-2; AW049765
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat, Cow
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
((0.1ug/ml) staining of Daudi (A) and Mouse Spleen (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
SRRT/ARS2, Polyclonal Antibody (Cat# AAA423653)
Full Name
Goat anti-SRRT/ARS2 Antibody
Gene Names
SRRT; ARS2; ASR2; serrate
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Sp1, Polyclonal Antibody (Cat# AAA423399)
Full Name
Goat anti-Sp1(mouse) Antibody
Gene Names
Sp1; Sp1-1; AA450830; AI845540; 1110003E12Rik
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat, Pig
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~33.7 kDa Cyclin D1 protein using Rabbit Anti-Cyclin D1 Polyclonal Antibody (SPC-731). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D1 Polyclonal Antibody (SPC-731) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~33.7 kDa. Other Band(s): ~40 kDa due to post translational modifications.)
Cyclin D1, Polyclonal Antibody (Cat# AAA8000590)
Full Name
Cyclin D1 antibody: ATTO 390
Gene Names
CCND1; BCL1; PRAD1; U21B31; D11S287E
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~21.6 kDa KRAS protein using Rabbit Anti-KRAS Polyclonal Antibody (SPC-777). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KRAS Polyclonal Antibody (SPC-777) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.6 kDa. Other Band(s): ~29 kDa (PTM).)
KRAS, Polyclonal Antibody (Cat# AAA8001429)
Full Name
KRAS Antibody: FITC
Gene Names
KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot detection of GAPDH(human specific) in SW480,A431,K562,Jurkat and Hela cell lysates using GAPDH(human specific) mouse mAb (1:5000 diluted). Predicted band size:37KDa.Observed band size:37KDa.)
GAPDH, Monoclonal Antibody (Cat# AAA9215589)
Full Name
GAPDH(human specific) Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human
Applications
WB
Pricing
$415/0.1 mL | $1,625/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~42.3 kDa Wnt5a protein using Rabbit Anti-Wnt5a Polyclonal Antibody (SPC-737). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody (SPC-737) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~42.3 kDa. Other Band(s): ~35 kDa isoform 2.)
Wnt5a, Polyclonal Antibody (Cat# AAA8000710)
Full Name
Wnt5a Antibody: HRP
Gene Names
WNT5A; hWNT5A
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody, Clone 12F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxynonenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
4-Hydroxynonenal, Monoclonal Antibody (Cat# AAA808711)
Full Name
4-Hydroxynonenal Antibody, Clone 12F7: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
7-Ketocholesterol, Monoclonal Antibody (Cat# AAA808718)
Full Name
7-Ketocholesterol Antibody, Clone 7E1
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.8 kDa c-Myc protein using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.8 kDa.)
c-Myc, Polyclonal Antibody (Cat# AAA8000483)
Full Name
c-Myc Antibody: ATTO 488
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (SPC-742). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 ug/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (SPC-742) at 1:500 for 24 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.)
Alpha Synuclein, Polyclonal Antibody (Cat# AAA8000799)
Full Name
Alpha Synuclein Antibody (pSer129): FITC
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human; Mouse
Applications
WB, EIA, IHC
Purity
Peptide Affinity Purified
Pricing
$550/0.1 mL | $2,235/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
7-Ketocholesterol, Monoclonal Antibody (Cat# AAA808752)
Full Name
7-Ketocholesterol Antibody, Clone 3F7: RPE
Reactivity
ALL
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,260/5x0.1 mg
DyLight488 Conjugated Goat IgG, Detection Kit (Cat# AAA177105)
Full Name
DyLight488 Conjugated Goat IgG SABC Kit
Applications
IHC, IHC, ICC
Pricing
$440/1 Kit | $1,965/5x1 Kit
