Rabbit TRB3 / TRIB3 Polyclonal Antibody | anti-TRB3 / TRIB3 antibody

Anti-TRB3 / TRIB3 Antibody (Internal) IHC-plus

Cat. Num. AAA2400246

• Gene Names: TRIB3; NIPK; SINK; TRB3; SKIP3; C20orf97
• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Peptide Column Affinity Chromatography
• Synonyms: TRB3 / TRIB3; Polyclonal Antibody; Anti-TRB3 / TRIB3 Antibody (Internal) IHC-plus; TRIB3; C20orf97; NIPK; SINK; TRB-3; TRB3; DJ1103G7.3; SKIP3; Tribbles homolog 3; anti-TRB3 / TRIB3 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRB3 antibody is human, mouse and rat reactive. At least two isoforms of TRB3 are known to exist; this antibody will detect both isoforms.
• Purity/Purification: Peptide Column Affinity Chromatography
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 358

• Applicable Applications for anti-TRB3 / TRIB3 antibody: Immunohistochemistry (IHC) - Paraffin, Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P (10 ug/ml); WB (1 - 2 ug/ml); ; (optimal dilution to be determined by the researcher); ; Usage Summary: TRB3 antibody can be used for detection of TRB3 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 39 kDa Observed: 39 kDa
• Host Note: TRB3 / TRIB3 antibody was produced in Rabbit
• Immunogen Species: TRB3 / TRIB3 antibody was raised against Human
• Antigen Type: Synthetic
• Immunogen: TRB3 / TRIB3 antibody was raised against tRB3 antibody was raised against a 17 amino acid peptide near the center of human TRB3. The immunogen is located within amino acids 100 - 150 of TRB3.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 31542265
• NCBI GeneID: 57761
• NCBI Accession #: NP_066981.2
• NCBI GenBank Nucleotide #: NM_021158.4
• UniProt Accession #: Q96RU7; Q53GU4; Q53ZW7; Q6I9Y9; Q8TAI6; Q9H5M8; Q9NUD2
• NCBI Official Full Name: tribbles homolog 3 isoform 1
• NCBI Official Synonym Full Names: tribbles pseudokinase 3
• NCBI Official Symbol: TRIB3
• NCBI Official Synonym Symbols: NIPK; SINK; TRB3; SKIP3; C20orf97
• NCBI Protein Information: tribbles homolog 3
• UniProt Protein Name: Tribbles homolog 3
• UniProt Gene Name: TRIB3
• UniProt Synonym Gene Names: C20orf97; NIPK; SKIP3; TRB3; TRB-3
• UniProt Entry Name: TRIB3_HUMAN

NCBI Description

The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TRB3: a Ca(2)/calmodulin-dependent protein kinase of the Trbl family. Induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. May negatively regulate the cell survival kinase AKT1.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 20p13-p12.2

Cellular Component: cytosol; nucleoplasm; nucleus; plasma membrane

Molecular Function: ATP binding; kinase activity; mitogen-activated protein kinase kinase binding; protein binding; protein kinase binding; protein kinase inhibitor activity; transcription corepressor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity

Biological Process: cellular lipid metabolic process; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; negative regulation of fat cell differentiation; negative regulation of fatty acid biosynthetic process; negative regulation of protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of ubiquitin-protein ligase activity; protein amino acid phosphorylation; regulation of MAP kinase activity; transcription, DNA-dependent