Western Blot (WB)
((0.3ug/ml) staining of A549 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat cytochrome P450 1A1 Polyclonal Antibody | anti-CYP1A1 antibody
Goat anti-cytochrome P450 1A1 Antibody
Western Blot: Approx 85-90kDa band observed in lysates of cell lines A549 and MCF7 (calculated MW of 58.2kDa according to NP_000490.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of A549 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of A549 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]
Uniprot Description
CYP1A1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.
Protein type: Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Amino Acid Metabolism - tryptophan; EC 1.14.14.1
Chromosomal Location of Human Ortholog: 15q24.1
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion
Molecular Function: demethylase activity; enzyme binding; flavonoid 3'-monooxygenase activity; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on diphenols and related substances as donors; oxygen binding; protein binding; steroid hydroxylase activity
Biological Process: 9-cis-retinoic acid biosynthetic process; aging; amine metabolic process; camera-type eye development; cell proliferation; coumarin metabolic process; dibenzo-p-dioxin catabolic process; drug metabolic process; embryonic development ending in birth or egg hatching; epoxygenase P450 pathway; ethylene metabolic process; flavonoid metabolic process; gut development; hydrogen peroxide biosynthetic process; insecticide metabolic process; maternal process involved in parturition; porphyrin metabolic process; response to antibiotic; response to arsenic; response to drug; response to food; response to herbicide; response to hyperoxia; response to hypoxia; response to iron(III) ion; response to lipopolysaccharide; response to nematode; response to virus; response to vitamin A; response to wounding; vitamin D metabolic process
Research Articles on CYP1A1
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Product Notes
The CYP1A1 cyp1a1 (Catalog #AAA423384) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-cytochrome P450 1A1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's cytochrome P450 1A1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 85-90kDa band observed in lysates of cell lines A549 and MCF7 (calculated MW of 58.2kDa according to NP_000490.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the CYP1A1 cyp1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CQEKQLDENA NVQ. It is sometimes possible for the material contained within the vial of "cytochrome P450 1A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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