Western Blot (WB)
(Western blot using Immunochemicals Affinity Purified Anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 _g of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (p/n B501-0500) and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reacting with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRP- conjugated a-Rabbit IgG (p/n 611-103-122) for 1 hour at room temperature. Detection was by ECL.)
Rabbit Smad1 Polyclonal Antibody
Smad1, phosphorylated (Ser206) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Smad1; Polyclonal Antibody; phosphorylated (Ser206) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma); Anti -Smad1; anti-Smad1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Smad1, phosphorylated at Ser206. Species Sequence Homology: duckbill platypus, drosophila, mouse, opossum, chimpanzee, bonobo, orangutan, gorilla, macaque, cattle, sheep, pig, rat, horse, salmon, chicken, and dog.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Applicable Applications for anti-Smad1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:25,000-1:30,000
Western Blot: 1:500
Dilution: ELISA: 1:25,000-1:30,000
Western Blot: 1:500
Immunogen
Synthetic phosphopeptide corresponding to the region of aa containing serine 206 of human SMAD1 protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot using Immunochemicals Affinity Purified Anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 _g of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (p/n B501-0500) and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reacting with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRP- conjugated a-Rabbit IgG (p/n 611-103-122) for 1 hour at room temperature. Detection was by ECL.)
Western Blot (WB)
(Western blot using Immunochemicals Affinity Purified Anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 _g of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (p/n B501-0500) and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reacting with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1:3,000 dilution HRP- conjugated a-Rabbit IgG (p/n 611-103-122) for 1 hour at room temperature. Detection was by ECL.)
Related Product Information for anti-Smad1 antibody
SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP
Product Categories/Family for anti-Smad1 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
~52kD
NCBI Official Full Name
SMAD1
Protein Family
Similar Products
Product Notes
The Smad1 (Catalog #AAA621285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Smad1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:25,000-1:30,000 Western Blot: 1:500. Researchers should empirically determine the suitability of the Smad1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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