Western Blot (WB)
((1ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Stap2 Polyclonal Antibody | anti-Stap2 antibody
Goat anti-Stap2 (mouse) Antibody
Gene Names
Stap2; STAP-2; AW049765
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Stap2; Polyclonal Antibody; Goat anti-Stap2 (mouse) Antibody; signal transducing adaptor family member 2; AW049765; STAP-2; signal-transducing adaptor protein 2; signal-transducing adaptor protein-2; AW049765 antibody; signal transducing adaptor family member 2 antibody; signal-transducing adaptor protein 2 antibody; signal-transducing adaptor protein-2 antibody; Stap2 antibody; STAP-2 antibody; Stap2 (mouse); anti-Stap2 antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EEKLQKRRALEH
Sequence Length
411
Applicable Applications for anti-Stap2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 50kDa band observed in Mouse fetal Brain and fetal Stomach lysates (calculated MW of 45.8kDa according to NP_666046.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Western Blot: Approx 50kDa band observed in Mouse fetal Brain and fetal Stomach lysates (calculated MW of 45.8kDa according to NP_666046.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-EEKLQKRRALEH, from the C Terminus of the protein sequence according to NP_666046.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,644 Da
NCBI Official Full Name
signal-transducing adaptor protein 2
NCBI Official Synonym Full Names
signal transducing adaptor family member 2
NCBI Official Symbol
Stap2
NCBI Official Synonym Symbols
STAP-2; AW049765
NCBI Protein Information
signal-transducing adaptor protein 2
UniProt Protein Name
Signal-transducing adaptor protein 2
Protein Family
UniProt Gene Name
Stap2
UniProt Synonym Gene Names
STAP-2
UniProt Entry Name
STAP2_MOUSE
Uniprot Description
STAP2: a widely expressed proimflammatory adaptor protein that regulates immune and inflammatory responses. Binds and modulates a variety of molecules including Brk, CSFR, FCER1, and BCR-ABL. The interaction between STAP-2 and BCR-ABL appears to provide a growth advantage to BCR-ABL+ chronic myeloid leukemia cells. Suppression of STAP-2 expression in K562 CML cells suppressed tumor formation in mice. Enables intestinal proinflammatory responses in vivo: promotes macrophage recruitment in dextran sodium sulfate induced colitis, and promotes the ability of CXCL12-induced migration in macrophages. STAP-2 knockdown in T47D breast cancer cells induced a significant decrease of proliferation. Contains a pleckstrin homology, Src homology 2-like, and as well as a STAT3-binding domains. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Cellular Component: cytoplasm; membrane; plasma membrane
Molecular Function: protein binding
Research Articles on Stap2
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Product Notes
The Stap2 stap2 (Catalog #AAA423298) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Stap2 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Stap2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 50kDa band observed in Mouse fetal Brain and fetal Stomach lysates (calculated MW of 45.8kDa according to NP_666046.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the Stap2 stap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EEKLQKRRAL EH. It is sometimes possible for the material contained within the vial of "Stap2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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