Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Sp1 Polyclonal Antibody | anti-Sp1 antibody
Goat anti-Sp1(mouse) Antibody
Western Blot: Approx 100kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 80.4kDa according to NP_038700.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
SP1: a transcription factor of the Sp1 C2H2-type zinc-finger protein family. Phosphorylated and activated by MAPK. Dephosphorylation by PTEN inhibits DNA binding. Binds to p38 in the nucleus. Interacts with Huntingtin and TAFII130. Transcriptional activity of SP1 and TAFII130 disrupted in early Huntingtin's Disease.
Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor
Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus
Molecular Function: bHLH transcription factor binding; DNA binding; double-stranded DNA binding; histone acetyltransferase binding; histone deacetylase binding; metal ion binding; nucleic acid binding; protein binding; protein C-terminus binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: embryonic camera-type eye morphogenesis; embryonic placenta development; embryonic process involved in female pregnancy; embryonic skeletal development; enucleate erythrocyte differentiation; in utero embryonic development; liver development; lung development; megakaryocyte differentiation; mRNA transcription from RNA polymerase II promoter; ossification; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; rhythmic process; transcription, DNA-dependent; trophectodermal cell differentiation
Research Articles on Sp1
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Product Notes
The Sp1 sp1 (Catalog #AAA423399) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Sp1(mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Sp1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 100kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 80.4kDa according to NP_038700.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the Sp1 sp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DGIHDETAGG EGEN. It is sometimes possible for the material contained within the vial of "Sp1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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