Western Blot (WB)
((2ug/ml) staining of Kidney (A), Testis (B) and Uterus (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TIM-1 Polyclonal Antibody | anti-HAVCR1 antibody
Goat anti-TIM-1 Antibody
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TIM-1; Polyclonal Antibody; Goat anti-TIM-1 Antibody; HAVCR1; hepatitis A virus cellular receptor 1; HAVCR; HAVCR-1; KIM-1; KIM1; TIM; TIM1; TIMD-1; TIMD1; OTTHUMP00000224005; OTTHUMP00000224052; OTTHUMP00000224053; T cell immunoglobin domain and mucin domain protein 1; T-cell membrane protein 1; kidn; HAVCR antibody; HAVCR1 antibody; HAVCR-1 antibody; hepatitis A virus cellular receptor 1 antibody; kidney injury molecule 1 antibody; KIM1 antibody; KIM-1 antibody; OTTHUMP00000224005 antibody; OTTHUMP00000224052 antibody; OTTHUMP00000224053 antibody; T cell immunoglobin domain and mucin domain protein 1 antibody; T-cell membrane protein 1 antibody; TIM antibody; TIM1 antibody; TIM-1 antibody; TIMD1 antibody; TIMD-1 antibody; anti-HAVCR1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
THVTYRKDTRYK
Sequence Length
1353
Applicable Applications for anti-HAVCR1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 52kDa band observed in Human Kidney, Testis and Uterus lysates (calculated MW of 39.2kDa according to NP_036338.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Western Blot: Approx 52kDa band observed in Human Kidney, Testis and Uterus lysates (calculated MW of 39.2kDa according to NP_036338.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-THVTYRKDTRYK, from the internal region of the protein sequence according to NP_036338.2.
Epitope
Internal region
Note
The immunizing peptide represents part of the extracellular domain. Reported variants represent identical protein: NP_001166864.1, NP_001092884.1, NP_036338.2
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((2ug/ml) staining of Kidney (A), Testis (B) and Uterus (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Kidney (A), Testis (B) and Uterus (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
157,011 Da
NCBI Official Full Name
TIM-1
NCBI Official Symbol
tim-1
NCBI Protein Information
Protein timeless homolog
UniProt Protein Name
Protein timeless homolog
Protein Family
UniProt Gene Name
tim-1
UniProt Entry Name
TIM_CAEEL
Uniprot Description
Plays an important role in chromosome cohesion during both mitosis and meiosis (PubMed:12827206). In prophase of meiosis, it is involved in the formation of the synaptonemal complex (SC) and specifically, in the diplotene and diakinesis phases of prophase, it stabilizes the association of homologous chromosomes during synapsis and sister chromatid cohesion (PubMed:12827206). It regulates cohesin subunits to promote meiotic chromosome cohesion and localizes non-SMC (structural maintenance of chromosome) cohesin subunits to chromatin prior to or during pre-meiotic S phase (PubMed:12827206). Implicated in influencing either the stability or loading of meiotic-specific cohesin subunit, rec8 (PubMed:12827206). Controls cell cycle exit and cell fusion to prevent the premature differentiation into adult cells (PubMed:15691769). Specifically, regulates hypodermal seam cell identity (PubMed:15691769).
Research Articles on HAVCR1
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Product Notes
The HAVCR1 tim-1 (Catalog #AAA423514) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TIM-1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIM-1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 52kDa band observed in Human Kidney, Testis and Uterus lysates (calculated MW of 39.2kDa according to NP_036338.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the HAVCR1 tim-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: THVTYRKDTR YK. It is sometimes possible for the material contained within the vial of "TIM-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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