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225 results for "major-cell-binding-factor" - showing 1-50
Sequence
Lysyl Oxidase (LOX), Active Protein (Cat# AAA2097305)
Full Name
Active Lysyl Oxidase (LOX)
Gene Names
LOX; AAT10
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>92%
Pricing
$220/0.01 mg | $460/0.05 mg | $705/0.1 mg | $875/0.2 mg | $1,705/0.5 mg | $2,540/1 mg | $6,205/5 mg
Western Blot (WB)
(Western blot detection of IkB-alpha (N-terminus) in Jurkat and Hela cell lysates using IkB-alpha (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.)
IkB-alpha, Monoclonal Antibody (Cat# AAA475019)
Full Name
Anti-IkB-alpha (N-term) Mouse mAb
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of IKB alpha (pS32/S36) expression in HepG2 TNFa-treated (A), Raw264.7 TNFa-treated (B), PC12 TNFa-treated (C) whole cell lysates.)
IKB alpha (pS32/S36), Polyclonal Antibody (Cat# AAA821495)
Full Name
Anti-IKB alpha (pS32/S36) Phospho Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat, Bovine, Pig, Sheep
Applications
WB, IHC, IF, ICC
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$185/0.03 mL | $275/0.1 mL | $380/0.2 mL | $1,650/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of Hela, Jurkat, 293T and K562 cell using NFKBIA Polyclonal Antibody at dilution of 1:84)
NFKBIA, Polyclonal Antibody (Cat# AAA2517661)
Full Name
NFKBIA Polyclonal Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Purity
Antigen affinity purification
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB)
(Western blot analysis of NFYA expression in A431 (A), K562 (B), NIH3T3 (C) whole cell lysates.)
IKB alpha, Polyclonal Antibody (Cat# AAA820453)
Full Name
Anti-IKB alpha Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Bovine, Monkey, Pig, Sheep
Applications
WB, IHC, IF, ICC, IP
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$175/0.03 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Activity Data
(Figure. The binding activity of APOA1 with Hpt.Apolipoprotein A1 (APOA1) is the major protein component of HDL particles in plasma. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA1 was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. ApoA1 is often used as a biomarker for prediction of cardiovascular diseases. Besides, Haptoglobin (Hpt) has been identified as an interactor of APOA1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APOA1 and recombinant human Hpt. Briefly, APOA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Hpt-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-APOA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of APOA1 and Hpt was shown in Figure 1, and this effect was in a dose dependent manner.)
Apolipoprotein A1 (APOA1), Active Protein (Cat# AAA2105095)
Full Name
Active Apolipoprotein A1 (APOA1)
Gene Names
APOA1; apo(a)
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Activity Data
(Figure. The binding activity of MIF with MHCDG.Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse MIF and recombinant mouse MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105113)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
Mif; GIF; DER6; Glif
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC4 Mouse Monoclonal Antibody (MUC4/3105).)
MUC4 (Mucin 4/Gastric Mucin), Monoclonal Antibody (Cat# AAA4381106)
Full Name
MUC4 (Mucin 4/Gastric Mucin)
Gene Names
MUC4; ASGP; MUC-4; HSA276359
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with AIF1 / Iba1 Mouse Monoclonal Antibody (AIF1/1909).)
AIF1/Iba1, Monoclonal Antibody (Cat# AAA439927)
Full Name
AIF1/Iba1 (Microglia Marker)
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Human. Others not known.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NFKBIA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
NFKBIA, Polyclonal Antibody (Cat# AAA126855)
Full Name
NFKBIA Polyclonal Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Human Lymph Node stained with AIF1 / Iba1 Mouse Recombinant Monoclonal Antibody (rAIF1/1909).)
AIF1/Iba1, Monoclonal Antibody (Cat# AAA439928)
Full Name
AIF1/Iba1 (Microglia Marker)
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Human. Others not known.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF)
(MBS9600622 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
I kappaB alpha, Polyclonal Antibody (Cat# AAA9600622)
Full Name
Phospho-I kappaB alpha (Ser32/Ser36) Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Hela whole cell lysates, using UHRF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
UHRF1, Polyclonal Antibody (Cat# AAA9607582)
Full Name
UHRF1 Antibody
Gene Names
UHRF1; Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, IP, EIA, ChIP
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Placenta stained with Ezrin Mouse Monoclonal Antibody (CPTC-Ezrin-1).)
Ezrin/p81, Monoclonal Antibody (Cat# AAA4380713)
Full Name
Ezrin/p81
Gene Names
EZR; CVL; CVIL; VIL2; HEL-S-105
Reactivity
Human. Others not tested.
Applications
WB, Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Kidney stained with AIF1 / Iba1 Mouse Monoclonal Antibody (AIF1/2493).)
AIF1/Iba1, Monoclonal Antibody (Cat# AAA439929)
Full Name
AIF1/Iba1 (Microglia Marker)
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Human. Others not known.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC4 Mouse Monoclonal Antibody (MUC4/3084).)
MUC4 (Mucin 4/Gastric Mucin), Monoclonal Antibody (Cat# AAA4381105)
Full Name
MUC4 (Mucin 4/Gastric Mucin)
Gene Names
MUC4; ASGP; MUC-4; HSA276359
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APEX Nuclease I Mouse Monoclonal Antibody (CPTC-APEX1-2).)
APEX Nuclease I, Monoclonal Antibody (Cat# AAA4380592)
Full Name
APEX Nuclease I
Gene Names
APEX1; APE; APX; APE1; APEN; APEX; HAP1; REF1
Reactivity
Human. Others not tested.
Applications
WB, Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Activity Data
(Figure. The binding activity of MIF with MHCDG.Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIF and recombinant human MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105112)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
MIF; GIF; GLIF; MMIF
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $510/0.05 mg | $790/0.1 mg | $975/0.2 mg | $1,905/0.5 mg | $2,820/1 mg | $6,895/5 mg
Immunofluorescence (IF)
(MBS9601010 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
I kappaB alpha, Polyclonal Antibody (Cat# AAA9601010)
Full Name
Phospho-I kappaB alpha (Tyr305) Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of IKB Alpha using anti- IKB Alpha antibody (MBS178205).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human MDA-MB-453 whole cell lysates,Lane 3: human SW620 whole cell lysates,Lane 4: human A549 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB Alpha antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IKB Alpha at approximately 39KD. The expected band size for IKB Alpha is at 36KD. )
IKB alpha, Polyclonal Antibody (Cat# AAA178205)
Full Name
Anti-IKB alpha Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of IKB alpha expression in HeLa (A), HepG2 (B), Raw264.7 (C) whole cell lysates.)
IKB alpha, Polyclonal Antibody (Cat# AAA821662)
Full Name
Anti-IKB alpha Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Bovine, Pig, Sheep
Applications
WB, IHC, IF, ICC
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$175/0.03 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells using RFXANK antibody (Red), compared to a nonspecific negative control antibody (Blue).)
RFXANK, Monoclonal Antibody (Cat# AAA832055)
Full Name
RFXANK antibody
Gene Names
RFXANK; BLS; RFX-B; ANKRA1; F14150_1
Applications
IF, FC/FACS, IHC, WB
Purity
RFXANK antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Immunofluorescence (IF)
(MBS9601829 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IkappaB-alpha, Polyclonal Antibody (Cat# AAA9601829)
Full Name
IkappaB-alpha Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
TAP1, Polyclonal Antibody (Cat# AAA9125900)
Full Name
TAP1 Polyclonal Antibody
Gene Names
TAP1; APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NFKBIA antibody.)
NFKBIA, Polyclonal Antibody (Cat# AAA2519002)
Full Name
NFKBIA Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Antigen affinity purification
Pricing
$405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Activity Data
(HIF1a (Hypoxia-inducible factor 1-alpha) is a subunit of a transcription factor, which functions as a master transcriptional regulator of the adaptive response to hypoxia. The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors, including HIF1a, thus a binding ELISA assay was conducted to detect the interaction of HIF1a and HSP90. Briefly, recombinant human HIF1a were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSP90-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-HIF1a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of HIF1a and HSP90 was shown in Figure 1, and this effect was in a dose dependent manner.)
Hypoxia Inducible Factor 1 Alpha (HIF1a), Active Protein (Cat# AAA2097345)
Full Name
Active Hypoxia Inducible Factor 1 Alpha (HIF1a)
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant RFXANK protein using RFXANK antibody)
RFXANK, Monoclonal Antibody (Cat# AAA833215)
Full Name
RFXANK antibody
Gene Names
RFXANK; BLS; RFX-B; ANKRA1; F14150_1
Applications
IF, IHC, WB
Purity
RFXANK antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western blot analysis of IkappaB-alpha expression in TNF-alpha treated MCF7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IkappaB-alpha, Polyclonal Antibody (Cat# AAA9601266)
Full Name
IkappaB-alpha Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of AIFM2 Antibody (C-term) (MBS9203952) in mouse liver tissue lysates (35ug/lane). AIFM2 (arrow) was detected using the purified Pab.)
AIFM2, Polyclonal Antibody (Cat# AAA9203952)
Full Name
AIFM2 Antibody (C-term)
Gene Names
AIFM2; AMID; PRG3
Reactivity
Human
(Predicted Reactivity: Bovine, Mouse)
(Predicted Reactivity: Bovine, Mouse)
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data #1
(Western blot analysis of hole cell lysate from HL60 promyelocytic leukemia cells probed with Rabbit anti Human adiponectin (MBS221314) at 0.5(A), 1(B) and 2(C) ug/ml)
ADIPONECTIN, Polyclonal Antibody (Cat# AAA221314)
Full Name
RABBIT ANTI ADIPONECTIN (C-TERMINAL)
Gene Names
ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Pricing
$540/0.1 mg | $2,280/5x0.1 mg
Western Blot (WB)
TAP1, Monoclonal Antibody (Cat# AAA375223)
Full Name
Mouse Anti-TAP1 [53H8]
Gene Names
TAP1; APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
Reactivity
Human
Applications
EIA, WB, IP, ICC, IF
Purity
Protein A
Pricing
$390/0.05 mL | $470/0.1 mL | $1,960/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells using RFXANK antibody (Red), compared to a nonspecific negative control antibody (Blue).)
RFXANK, Monoclonal Antibody (Cat# AAA833832)
Full Name
RFXANK antibody
Gene Names
RFXANK; BLS; RFX-B; ANKRA1; F14150_1
Applications
IF, FC/FACS, IHC, WB
Purity
RFXANK antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NELFE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
NELFE, Polyclonal Antibody (Cat# AAA9126596)
Full Name
NELFE Polyclonal Antibody
Gene Names
NELFE; RD; RDP; RDBP; D6S45; NELF-E
Reactivity
Human
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Activity Data
(Macrophage migration inhibitory factor (MIF), also known as glycosylation- inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein classified as an inflammatory cytokine. MIF is an important regulator of innate immunity. It involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. Besides, Major Histocompatibility Complex Class II Invariant Chain (MHCDG) has been identified as an interactor of MIF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MIF and recombinant human MHCDG. Briefly, MIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCDG- coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MIF and MHCDG was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of MIF with MHCDG.)
Macrophage Migration Inhibitory Factor (MIF), Active Protein (Cat# AAA2105111)
Full Name
Active Macrophage Migration Inhibitory Factor (MIF)
Gene Names
MIF; GIF; GLIF; MMIF
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $510/0.05 mg | $790/0.1 mg | $975/0.2 mg | $1,905/0.5 mg | $2,820/1 mg | $6,895/5 mg
Western Blot (WB)
(Western blot analysis of extracts of HeLa cell lines, using Phospho-NFKBIA-Y42 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
NFKBIA-Y42, Antibody (Cat# AAA128546)
Full Name
Phospho-NFKBIA-Y42 Polyclonal Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$260/0.05 mL | $385/0.1 mL | $570/0.2 mL
Western Blot (WB)
(Western blot analysis of HL60 cell lysate using NFKBIA antibody.)
NFKBIA, Polyclonal Antibody (Cat# AAA9410340)
Full Name
NFKBIA Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
$300/0.05 mL | $390/0.1 mL | $1,610/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells using RFXANK antibody (Red), compared to a nonspecific negative control antibody (Blue).)
RFXANK, Monoclonal Antibody (Cat# AAA833582)
Full Name
RFXANK antibody
Gene Names
RFXANK; BLS; RFX-B; ANKRA1; F14150_1
Applications
IF, FC/FACS, IHC, WB
Purity
RFXANK antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant RFXANK protein using RFXANK antibody)
RFXANK, Monoclonal Antibody (Cat# AAA834613)
Full Name
RFXANK antibody
Gene Names
RFXANK; BLS; RFX-B; ANKRA1; F14150_1
Applications
IF, IHC, WB
Purity
RFXANK antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NELFE antibody.)
NELFE, Polyclonal Antibody (Cat# AAA2555804)
Full Name
NELFE Polyclonal Antibody
Gene Names
NELFE; RD; RDP; RDBP; D6S45; NELF-E
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB)
(Western blot analysis of NFKBIA-S32/36 in Hela cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.)
NFKBIA, Polyclonal Antibody (Cat# AAA9213037)
Full Name
NFKBIA Antibody (S32/36)
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human (Predicted Reactivity: Mouse, Pig)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Ku80, Polyclonal Antibody (Cat# AAA222869)
Full Name
GOAT ANTI HUMAN Ku80
Gene Names
XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
Applications
EIA, WB
Pricing
$625/0.1 mg | $2,670/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
Y box binding protein 1, ELISA Kit (Cat# AAA939527)
Full Name
Human Nuclease-sensitive element-binding protein 1, YBX1 ELISA Kit
Gene Names
YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1
Reactivity
Human
Pricing
$530/48-Strip-Wells | $755/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,875/5x96-Strip-Wells | $5,480/10x96-Strip-Wells
Testing Data
Epidermal Growth Factor Receptor (EGFR), phospho-Tyr 1173 + Positive Control, Monoclonal Antibody (Cat# AAA311353)
Full Name
MAb to EGFR, Activated pTyr 1173
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human
Applications
EIA/ELISA, Immunofluorescence Assay, Immunoprecipitation, Western Blot
Purity
Thiophilic adsorption and size exclusion chromatography
Pricing
$1,085/0.1 mg | $4,725/5x0.1 mg
IL-6, Polyclonal Antibody (Cat# AAA220073)
Full Name
RABBIT ANTI CHICKEN INTERLEUKIN-6:Preservative Free
Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Applications
EIA, FN, WB
Pricing
$610/0.5 mg | $2,585/5x0.5 mg
CTGF, Active Protein (Cat# AAA538277)
Full Name
CTGF protein
Gene Names
CTGF; CCN2
Applications
User optimized
Purity
> 95% pure
Pricing
$425/0.02 mg | $1,905/5x0.02 mg
Western Blot (WB)
()
IkB alpha, Monoclonal Antibody (Cat# AAA833360)
Full Name
IkB alpha antibody
Applications
EIA, WB
Purity
IkB alpha antibody was purified by ammonium sulfate precipitation
Western Blot (WB)
(Anti- YBX1 antibody, MBS175269, Western blottingAll lanes: Anti YBX1 (MBS175269) at 0.5ug/mlWB: HT1080Whole Cell Lysate at 40ugPredicted bind size: 50KDObserved bind size: 50KD )
YB1, Polyclonal Antibody (Cat# AAA175269)
Full Name
Anti-YB1 antibody
Gene Names
YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
IGF BP4, Active Protein (Cat# AAA539608)
Full Name
IGF BP4 protein
Gene Names
IGFBP4; BP-4; IBP4; IGFBP-4; HT29-IGFBP
Applications
User optimized
Purity
> 95% pure
Pricing
$425/0.02 mg | $1,905/5x0.02 mg
CTGF, Polyclonal Antibody (Cat# AAA535951)
Full Name
CTGF antibody
Gene Names
CTGF; CCN2; NOV2; HCS24; IGFBP8
Reactivity
Human CTGF
Applications
EIA, WB
Pricing
$525/0.1 mg | $2,355/5x0.1 mg
IGF BP5, Active Protein (Cat# AAA539557)
Full Name
IGF BP5 protein
Gene Names
IGFBP5; IBP5
Applications
User optimized
Purity
> 98% pure
Pricing
$425/0.025 mg | $1,905/5x0.025 mg
