Rabbit NFKBIA Polyclonal Antibody | anti-NFKBIA antibody

NFKBIA Antibody (S32/36)

Cat. Num. AAA9213037

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Human (Predicted Reactivity: Mouse, Pig)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: NFKBIA; Polyclonal Antibody; NFKBIA Antibody (S32/36); NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; IKBA; MAD3; NFKBI; anti-NFKBIA antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse, Pig)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from human NFKBIA.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 12-41
• Sequence Length: 317

• Applicable Applications for anti-NFKBIA antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of NFKBIA-S32/36 in Hela cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using NFKBIA Antibody (S32/36)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NFKBIA Antibody (S32/36) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

Related Product Information for anti-NFKBIA antibody

NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Product Categories/Family for anti-NFKBIA antibody

Cancer; Cell Biology; Immunology; Signal Transduction

References

Szamosi,T., Dig. Dis. Sci. (2008)
Fan,C., J. Biol. Chem. 278 (3), 2072-2080 (2003)
Tojima,Y., Nature 404 (6779), 778-782 (2000)
Gil,J., Oncogene 19 (11), 1369-1378 (2000)
Hay,R.T., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 354 (1389), 1601-1609(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390.1
• NCBI GenBank Nucleotide #: NM_020529.2
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 35609
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha
• UniProt Entry Name: IKBA_HUMAN

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

IkB-alpha: a regulatory protein that inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation.

Protein type: DNA-binding; Inhibitor

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: identical protein binding; NF-kappaB binding; protein binding; enzyme binding; ubiquitin protein ligase binding; heat shock protein binding; nuclear localization sequence binding; protein complex binding; transcription factor binding

Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; apoptosis; positive regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; cytoplasmic sequestering of NF-kappaB; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; cytoplasmic sequestering of transcription factor; negative regulation of myeloid cell differentiation; MyD88-independent toll-like receptor signaling pathway; negative regulation of DNA binding; regulation of NF-kappaB import into nucleus; toll-like receptor 2 signaling pathway; regulation of cell proliferation; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; response to muramyl dipeptide; protein import into nucleus, translocation; toll-like receptor signaling pathway; negative regulation of Notch signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of apoptosis

Disease: Ectodermal Dysplasia, Anhidrotic, With T-cell Immunodeficiency, Autosomal Dominant