Human Y box binding protein 1 ELISA Kit | YBX1 elisa kit

Human Nuclease-sensitive element-binding protein 1, YBX1 ELISA Kit

Cat. Num. AAA939527

• Gene Names: YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1
• Reactivity: Human
• Synonyms: Y box binding protein 1; Human Nuclease-sensitive element-binding protein 1; YBX1 ELISA Kit; Human Nuclease-sensitive element-binding protein 1 (YBX1) ELISA kit; BP-8; CSDA2; CSDB; DBPB; MDR-NF1; MGC104858; MGC110976; MGC117250; NSEP-1; NSEP1; YB-1; YB1; DNA-binding protein B; major histocompatibility complex; class II; Y box-binding protein I; nuclease sensit; YBX1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human YBX1. No significant cross-reactivity or interference between human YBX1 and analogues was observed.
• Sequence Length: 324

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: < 7.81 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for YBX1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YBX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YBX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YBX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YBX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 34098946
• NCBI GeneID: 4904
• NCBI Accession #: NP_004550.2
• NCBI GenBank Nucleotide #: NM_004559.3
• UniProt Accession #: P67809; P16990; P16991; Q14972; Q15325; Q5FVF0
• Molecular Weight: 35,924 Da
• NCBI Official Full Name: nuclease-sensitive element-binding protein 1
• NCBI Official Synonym Full Names: Y box binding protein 1
• NCBI Official Symbol: YBX1
• NCBI Official Synonym Symbols: YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1
• NCBI Protein Information: nuclease-sensitive element-binding protein 1; CBF-A; EFI-A; DNA-binding protein B; Y-box-binding protein 1; Y-box transcription factor; enhancer factor I subunit A; nuclease sensitive element binding protein 1; CCAAT-binding transcription factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I
• UniProt Protein Name: Nuclease-sensitive element-binding protein 1
• Protein Family: Nuclease-sensitive element-binding protein
• UniProt Gene Name: YBX1
• UniProt Synonym Gene Names: NSEP1; YB1; CBF-A; DBPB; EFI-A; YB-1
• UniProt Entry Name: YBOX1_HUMAN

Uniprot Description

YB-1: a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood.

Protein type: RNA processing; Transcription factor; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: nucleoplasm; nuclear membrane; intracellular membrane-bound organelle; stress granule; cytoplasm; ribonucleoprotein complex; nucleus; U12-dependent spliceosome

Molecular Function: protein binding; DNA binding; RNA binding; double-stranded DNA binding; transcription factor activity; single-stranded DNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of striated muscle cell differentiation; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; in utero embryonic development; positive regulation of cell division; RNA splicing; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter