Mouse RFXANK Monoclonal Antibody | anti-RFXANK antibody

RFXANK antibody

Cat. Num. AAA833215

• Gene Names: RFXANK; BLS; RFX-B; ANKRA1; F14150_1
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: RFXANK antibody was purified by affinity chromatography.
• Synonyms: RFXANK; Monoclonal Antibody; RFXANK antibody; Monoclonal RFXANK; Anti-RFXANK; ANKRA1; BLS; F14150_1; MGC138628; RFX-B; anti-RFXANK antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 2B6
• Purity/Purification: RFXANK antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5-1 mg/ml (varies by lot)
• Sequence Length: 260

• Applicable Applications for anti-RFXANK antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:150; WB: 1:500; IF: 1:100
• Biological Significance: Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B.
• Cross-Reactivity: Human
• Immunogen: RFXANK antibody was raised in Mouse using full length human recombinant protein of human RFXANK produced in HEK293T cell as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant RFXANK protein using RFXANK antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RFXANK protein (Right) or empty vector (Left) detected with RFXANK antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of RFXANK protein in paraffin embedded Adenocarcinoma of Human colon tissue using RFXANK antibody)

Related Product Information for anti-RFXANK antibody

Mouse monoclonal RFXANK antibody

Product Categories/Family for anti-RFXANK antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 3820616
• NCBI GeneID: 8625
• UniProt Accession #: O14593; O95839; Q24JQ1; Q6FGA8
• Molecular Weight: 25,652 Da
• NCBI Official Full Name: RFXANK
• NCBI Official Synonym Full Names: regulatory factor X-associated ankyrin-containing protein
• NCBI Official Symbol: RFXANK
• NCBI Official Synonym Symbols: BLS; RFX-B; ANKRA1; F14150_1
• NCBI Protein Information: DNA-binding protein RFXANK
• UniProt Protein Name: DNA-binding protein RFXANK
• Protein Family: DNA-binding protein
• UniProt Gene Name: RFXANK
• UniProt Synonym Gene Names: ANKRA1; RFXB; RFX-B
• UniProt Entry Name: RFXK_HUMAN

NCBI Description

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

RFXANK: Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (MHC2TA). May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. Isoform RFX-B-delta5 is not involved in the positive regulation of MHC class II genes. Defects in RFXANK are a cause of bare lymphocyte syndrome type 2 (BLS2); also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: nucleoplasm; intercellular bridge; cytoplasm

Molecular Function: DNA binding; histone deacetylase binding; transcription cofactor activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; Ras protein signal transduction; positive regulation of transcription from RNA polymerase II promoter

Disease: Bare Lymphocyte Syndrome, Type Ii